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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K

Authors: Zhengyue Chen, Yanyan Yuan, Xinrong Zou, Mengqi Hong, Ming Zhao, Yu Zhao, Yuanping Liu, Guofu Li, Yabin Zhu, Lin Luo, Beiyan Bao, Shizhong Bu

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

Radix puerariae (RP) is a herbal medicines for diabetes, mainly because of anti-oxidative, insulin resistance and hypoglycemic effect. Fructus crataegi (FC) also possesses strong antioxidant activity in vitro. This study focused on the effects of herbal mixture of RP and FC (RPFC) on renal protection through a diabetic rat model.

Methods

Type 2 Diabetic model was established with high fat diet followed by injecting rats a low dose of STZ (25 mg/kg body weight). Rats were randomly divided into five groups: normal, high fat diet, diabetes mellitus, high fat diet plus RPFC prevention, and RPFC prevention before diabetes mellitus. RPFC was given to rats daily by intragastric gavage. The blood bio-chemical index and renal pathological changes were examined. The later includes hematoxylin and eosin staining, periodic acid schiff staining, and Masson trichrome staining. Protein levels of were determined by Western blot and immunohistochemical staining. mRNA levels were detected by RT-PCR.

Results

Rats prevented with RPFC resulted in decreasing blood glucose with corresponding vehicle treated rats. Glomerulus mesangial matrix expansion, renal capsule constriction, and renal tubular epithelial cell edema were less severe following RPFC prevention. Moreover, RPFC prevention reduced protein levels of PI3K, AKT, α-SMA and collagen IV in the kidney of diabetic rats.

Conclusion

Combined prevention with RPFC may inhibit the PI3K/AKT pathway in the kidney, thereby prevent renal injury in diabetic rats.
Literature
1.
Hosseini SM, Boright AP, Sun L, Canty AJ, Bull SB, Klein BE, Klein R, Group DER, Paterson AD. The association of previously reported polymorphisms for microvascular complications in a meta-analysis of diabetic retinopathy. Hum Genet. 2015;134(2):247–57.CrossRefPubMed
2.
Dalla Vestra M, Saller A, Mauer M, Fioretto P. Role of mesangial expansion in the pathogenesis of diabetic nephropathy. J Nephrol. 2001;14(Suppl 4):S51–7.PubMed
3.
Herman-Edelstein M, Scherzer P, Tobar A, Levi M, Gafter U. Altered renal lipid metabolism and renal lipid accumulation in human diabetic nephropathy. J Lipid Res. 2014;55(3):561–72.CrossRefPubMedPubMedCentral
4.
Kanauchi M, Ishii K, Nishiura K, Dohi K. Effect of exercise on hemodynamics and urinary protein excretion in patients with early-stage diabetic nephropathy. Nihon Jinzo Gakkai Shi. 1994;36(8):928–33.PubMed
5.
6.
Ma J, Wu H, Zhao CY, Panchapakesan U, Pollock C, Chadban SJ. Requirement for TLR2 in the development of albuminuria, inflammation and fibrosis in experimental diabetic nephropathy. Int J Clin Exp Pathol. 2014;7(2):481–95.PubMedPubMedCentral
7.
Fu X, Song B, Tian GW, Li JL. The effects of the water-extraction of Astragali radix and Lycopi herba on the pathway of TGF-smads-UPP in a rat model of diabetic nephropathy. Pharmacogn Mag. 2014;10(40):491–6.CrossRefPubMedPubMedCentral
8.
Hou Q, Ao X, Li G, Zhang Y. Puerarin combined with avandia for diabetic nephropathy. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012;37(1):73–7.PubMed
9.
Jin G, Yang P, Gong Y, Fan X, Tang J, Lin J. effects of puerarin on expression of apelin and its receptor of 2K1C renal hypertension rats. Zhongguo Zhong Yao Za Zhi. 2009;34(24):3263–7.PubMed
10.
Zhao Y, Zhou Y. Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path. Chin J Integr Med. 2012;18(4):293–8.CrossRefPubMed
11.
Chen J, Zhao H, Yang Y, Liu B, Ni J, Wang W. Lipid-lowering and antioxidant activities of Jiang-Zhi-Ning in traditional Chinese medicine. J Ethnopharmacol. 2011;134(3):919–30.CrossRefPubMed
12.
Chen TR, Chen LA, Wei QK. Evaluation of quality of radix Puerariae herbal medicine by isoflavonoids. J Pharm Pharmacol. 2010;62(5):644–50.CrossRefPubMed
13.
Sugano M, Yamato H, Hayashi T, Ochiai H, Kakuchi J, Goto S, Nishijima F, Iino N, Kazama JJ, Takeuchi T, et al. High-fat diet in low-dose-streptozotocin-treated heminephrectomized rats induces all features of human type 2 diabetic nephropathy: a new rat model of diabetic nephropathy. Nutr Metab Cardiovasc Dis. 2006;16(7):477–84.CrossRefPubMed
14.
Zhang H, Zhao T, Gong Y, Dong X, Zhang W, Sun S, Wang H, Gu Y, Lu X, Yan M, et al. Attenuation of diabetic nephropathy by Chaihuang-Yishen granule through anti-inflammatory mechanism in streptozotocin-induced rat model of diabetics. J Ethnopharmacol. 2014;151(1):556–64.CrossRefPubMed
15.
Tancrede G, Rousseau-Migneron S, Nadeau A. Long-term changes in the diabetic state induced by different doses of streptozotocin in rats. Br J Exp Pathol. 1983;64(2):117–23.PubMedPubMedCentral
16.
Jung CH, Jung SH, Kim KJ, Kim BY, Kim CH, Kang SK, Mok JO. Differential associations of central and brachial blood pressure with carotid atherosclerosis and microvascular complications in patients with type 2 diabetes. BMC Cardiovasc Disord. 2014;14:23.CrossRefPubMedPubMedCentral
17.
Xu Z, Ju J, Wang K, Gu C, Feng Y. Evaluation of hypoglycemic activity of total lignans from Fructus Arctii in the spontaneously diabetic Goto-Kakizaki rats. J Ethnopharmacol. 2014;151(1):548–55.CrossRefPubMed
18.
Koppelman MH, Cuypers HT, Emrich T, Zaaijer HL. Quantitative real-time detection of parvovirus B19 DNA in plasma. Transfusion. 2004;44(1):97–103.CrossRefPubMed
19.
Zhang W, Zhao L, Su SQ, Xu XX, Wu YG. Total glucosides of paeony attenuate renal tubulointerstitial injury in STZ-induced diabetic rats: role of toll-like receptor 2. J Pharmacol Sci. 2014;125(1):59–67.CrossRefPubMed
20.
Obeidat M, Li L, Ballermann BJ. TIMAP promotes angiogenesis by suppressing PTEN-mediated Akt inhibition in human glomerular endothelial cells. Am J Physiol Renal Physiol. 2014;307(5):F623–33.CrossRefPubMed
21.
Ren F, Tang L, Cai Y, Yuan X, Huang W, Luo L, Zhou J, Zheng Y. Meta-analysis: the efficacy and safety of combined treatment with ARB and ACEI on diabetic nephropathy. Ren Fail. 2015;37(4):548–61.CrossRefPubMed
22.
She S, Liu W, Li T, Hong Y. Effects of puerarin in STZ-induced diabetic rats by oxidative stress and the TGF-beta1/Smad2 pathway. Food Funct. 2014;5(5):944–50.CrossRefPubMed
23.
Song CY, Bi HM. effects of puerarin on plasma membrane GLUT4 content in skeletal muscle from insulin-resistant Sprague-Dawley rats under insulin stimulation. Zhongguo Zhong Yao Za Zhi. 2004;29(2):172–5.PubMed
24.
Jim B, Santos J, Spath F, Cijiang He J. Biomarkers of diabetic nephropathy, the present and the future. Curr Diabetes Rev. 2012;8(5):317–28.CrossRefPubMed
25.
Liu LT, Zheng GJ, Zhang WG, Guo G, Wu M. clinical study on treatment of carotid atherosclerosis with extraction of polygoni cuspidati rhizoma et radix and crataegi fructus: a randomized controlled trial. Zhongguo Zhong Yao Za Zhi. 2014;39(6):1115–9.PubMed
26.
Villanueva S, Contreras F, Tapia A, Carreno JE, Vergara C, Ewertz E, Cespedes C, Irarrazabal C, Sandoval M, Velarde V, et al. Basic fibroblast growth factor reduces functional and structural damage in chronic kidney disease. Am J Physiol Renal Physiol. 2014;306(4):F430–41.CrossRefPubMed
27.
Niu H, Nie L, Liu M, Chi Y, Zhang T, Li Y. Benazepril affects integrin-linked kinase and smooth muscle alpha-actin expression in diabetic rat glomerulus and cultured mesangial cells. BMC Nephrol. 2014;15:135.CrossRefPubMedPubMedCentral
28.
Liu P, Li F, Qiu M, He L. Expression and cellular distribution of TLR4, MyD88, and NF-kappaB in diabetic renal tubulointerstitial fibrosis, in vitro and in vivo. Diabetes Res Clin Pract. 2014;105(2):206–16.CrossRefPubMed
29.
Suwanpen C, Nouanthong P, Jaruvongvanich V, Pongpirul K, Pongpirul WA, Leelahavanichkul A, Kanjanabuch T. Urinary podocalyxin, the novel biomarker for detecting early renal change in obesity. J Nephrol. 2016;29(1):37–44.CrossRefPubMed
30.
Zeisberg M, Ericksen MB, Hamano Y, Neilson EG, Ziyadeh F, Kalluri R. Differential expression of type IV collagen isoforms in rat glomerular endothelial and mesangial cells. Biochem Biophys Res Commun. 2002;295(2):401–7.CrossRefPubMed
31.
Korish AA, Abdel Gader AG, Korashy HM, Al-Drees AM, Alhaider AA, Arafah MM. Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: inhibition of Smad1 and collagen type IV synthesis. Chem Biol Interact. 2015;229:100–8.CrossRefPubMed
32.
Zhong Y, Zhang X, Cai X, Wang K, Chen Y, Deng Y. Puerarin attenuated early diabetic kidney injury through down-regulation of matrix metalloproteinase 9 in streptozotocin-induced diabetic rats. PLoS One. 2014;9(1):e85690.CrossRefPubMedPubMedCentral
Metadata
Title
Radix Puerariae and Fructus Crataegi mixture inhibits renal injury in type 2 diabetes via decreasing of AKT/PI3K
Authors
Zhengyue Chen
Yanyan Yuan
Xinrong Zou
Mengqi Hong
Ming Zhao
Yu Zhao
Yuanping Liu
Guofu Li
Yabin Zhu
Lin Luo
Beiyan Bao
Shizhong Bu
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1945-3

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