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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Cell cycle arrest and apoptosis induction by methanolic leaves extracts of four Annonaceae plants

Authors: Kitti Pumiputavon, Tanawat Chaowasku, Chalermpong Saenjum, Maslin Osathanunkul, Boonsong Wungsintaweekul, Kriangkrai Chawansuntati, Jiraprapa Wipasa, Pathrapol Lithanatudom

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders, Artabotrys burmanicus A.DC, Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders and Dasymaschalon sp. have been used for traditional medicine to treat cancer-like symptoms in some ethnic groups of Thailand and Laos.

Methods

We evaluated the anti-cancer activity of these Annonaceae plants against several human cancer cell lines. The apoptosis induction was detected by Annexin/propidium iodide (PI) staining. Phytochemical screening was tested by standard protocols and bioactive compounds were determined by HPLC.

Results

The crude extracts from leaves of U. longipes, Dasymaschalon sp., A. burmanicus, and M. modestum showed particular effects that were found to vary depending on the cancer cell line, suggesting that the effect was in a cell-type specific manner. Interestingly, the induction of apoptotic cell death was prominent by the leaves-derived crude extract of M. modestum. This crude was, therefore, subjected to cell cycle analysis by PI staining. Results showed that this crude extract arrested cell cycle and increased the percentage of cells in the SubG1 phase in some cancer cell lines. The phytochemical screening tests indicated that all crude extracts contained tannins and flavonoids. HPLC of flavonoids using standards identified rutin as an active compound in U. longipes and Dasymaschalon sp., whereas quercetin was found in U. longipes and M. modestum.

Conclusions

These crude extracts provide a new source for rutin and quercetin, which might be capable of inducing cancer cell apoptotic death in a cell-type specific manner. This suggests, by analyzing the major bioactive compounds, the potential use of these crudes for chemotherapy in the future.
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Metadata
Title
Cell cycle arrest and apoptosis induction by methanolic leaves extracts of four Annonaceae plants
Authors
Kitti Pumiputavon
Tanawat Chaowasku
Chalermpong Saenjum
Maslin Osathanunkul
Boonsong Wungsintaweekul
Kriangkrai Chawansuntati
Jiraprapa Wipasa
Pathrapol Lithanatudom
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1811-3

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