Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Punica granatum peel extracts: HPLC fractionation and LC MS analysis to quest compounds having activity against multidrug resistant bacteria

Authors: Ilyas Khan, Hazir Rahman, Nasser M. Abd El-Salam, Abdul Tawab, Anwar Hussain, Taj Ali Khan, Usman Ali Khan, Muhammad Qasim, Muhammad Adnan, Azizullah Azizullah, Waheed Murad, Abdullah Jalal, Noor Muhammad, Riaz Ullah

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

Login to get access

Abstract

Background

Medicinal plants are rich source of traditional herbal medicine around the globe. Most of the plant’s therapeutic properties are due to the presence of secondary bioactive compounds.

Methods

The present study analyzed the High Pressure Liquid Chromatography (HPLC) fractions of Puncia granatum (peel) extracts (aqueous, chloroform, ethanol and hexane) against multidrug resistant bacterial pathogens (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus). All the fractions having antibacterial activity was processed for bioactive compounds identification using LC MS/MS analysis.

Results

Among total HPLC fractions (n = 30), 4 HPLC fractions of P. granatum (peel) showed potential activity against MDR pathogens. Fraction 1 (F1) and fraction 4 (F4) collected from aqueous extract showed maximum activity against P. aeruginosa. Fraction 2 (F2) of hexane showed antibacterial activity against three pathogens, while ethanol F4 exhibited antibacterial activity against A. baumannii. The active fractions were processed for LC MS/MS analysis to identify bioactive compounds. Valoneic acid dilactone (aqueous F1 and F4), Hexoside (ethanol F4) and Coumaric acid (hexane F2) were identified as bioactive compounds in HPLC fractions.

Conclusion

Puncia granatum peel extracts HPLC fractions exhibited potential inhibitory activity against MDR bacterial human pathogens. Several bioactive compounds were identified from the HPLC fractions. Further characterization of these compounds may be helpful to conclude it as therapeutic lead molecules against MDR pathogens.
Literature
1.
Aisha A, Sarfraz RA, Mahmood A, Din MU. Chemical composition and in vitro antioxidant and antitumor activities of Eucalyptus camaldulensis Dehn. leaves. Ind Crop Prod. 2015;74:241–8.CrossRef
2.
Tahir HU, Sarfraz AR, Aisha A, Adil S. Chemical composition and anti-diabetic activity of essential oils obtained from two spices (Syzygium aromaticum and Cuminum cyminum). Int J Food Prop. 2016;19:2156–64.CrossRef
3.
Prajapati ND, Prajapati T. Sustainable cultivation of medicinal plants; Multi tier agriculture system-A new concept. Mathews CE, Van Holde KE, Ahern KG. Biochemistry. 3rd edn. USA: Benjamin Cummings. 1999.
4.
Zafar R. Medicinal plants of India. 15th ed. Delhi: CBS Publication; 1994.
5.
Rauf A, Muhammad N, Khan A, Uddin N, Atif M, Barkatullah. Antibacterial and Phytotoxic Profile of Selected Pakistani Medicinal Plants. World App Sci J. 2012;20:540–4.
6.
Gordon MC, David JN. Natural product drug discovery in the next millennium. Pharm Bio. 2001;39:8–17.
7.
Shahwar D, Raza MA. In vitro antibacterial activity of extracts of Mimusops elengi against gram positive and gram negative bacteria. Afr Jo Micro Res. 2009;3:458–62.
8.
Reddy MK, Gupta SK, Jacob MR, Khan SI, Ferreira D. Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L. J Med Plant Nat Prod Res. 2007;73:461–7.CrossRef
9.
Dahham SS, Hajera MN, Tabassum A, Khan M. Studies on Antibacterial and antifungal activity of Pomegranate (Punica granatum L.). Am Eurasian J Agri Envir Sci. 2010;9:273–81.
10.
Wendakoon C, Calderon P, Gagnon D. Evaluation of selected medicinal plants extracted in different ethanol concentrations for antibacterial activity against human pathogens. J Med Act Plants. 2012;1:60–8.
11.
Odey MO, Iwara IA, Udiba UU, Johnson JT, Inekwe UV, Asenye ME, Victor O. Preparation of plant extracts from indigenous medicinal plants. Int J Sci Tech. 2012;1:688–92.
12.
Cock IE. Problems of Reproducibility and Efficacy of Bioassays Using Crude Extracts, with reference to Aloe vera. Pharm Comm. 2011;1:52–62.
13.
Khan UA, Rahman H, Qasim M, Hussain A, Azizullah A, Murad W, Khan Z, Anees M, Adnan M. Alkanna tinctoria leaves extracts: a prospective remedy against multidrug resistant human pathogenic bacteria. BMC Comp Alt Med. 2015;15:127.CrossRef
14.
Valgas C, de-Souza SM, EFA S, Smânia A Jr. Screening methods to determine antibacterial activity of natural products. Braz J Micro. 2007;38:369–80.CrossRef
15.
Steinmann D, Ganzera M. Recent advances on HPLC/MS in medicinal plant analysis. J Pharm Biomed Anal. 2011;55:744–57.CrossRefPubMed
16.
Garcia-Sosa K, Villarreal-Alvarez N, Lubben P, Peña-Rodriguez LM. Chrysophanol, an antimicrobial anthraquinone from the root extract of Colubrina greggii. J Mex Chem Soci. 2006;50:76–8.
17.
Prajna J, Richa J, Dipjyoti C. HPLC Quantification of Phenolic Acids from Vetiveria zizanioides (L.) Nash and Its Antioxidant and Antimicrobial Activity. J Pharm. 2013;6:1–6.
18.
Martinsa S, Elba ALC, Sobrinhob TJSP, Saraivac AM, Pisciottanoc MNC, Aguilard CN, Teixeiraa JA, Mussattoa SI. Antibacterial activity of crude methanolic extract and fractions obtained from Larrea tridentata leaves. Ind Crops Prod. 2012;41:306–11.CrossRef
19.
Fatema N, Sabir A, Kamrunnahar. Evaluation of antimicrobial, antioxidant and cytotoxic activities of methanolic extracts of Lagerstroemia speciosa leaves and barks. J Appl Pharm Sci. 2012;2:142–7.
20.
Ibrahima RM, Ali ME, Dalia OS, Naggar EMBE, Abd El-Rahman OE, Seham SE. HPLC-DAD-MS/MS profiling of phenolics from Securigera securidaca flowers and its anti-hyperglycemic and anti-hyperlipidemic activities. Rev Bras. 2015;25:134–41.
21.
Katarina R, Silvia F, Lucia J, Pavel M, Lívia S. The Activity of Cotinus coggygria Scop. Leaves Extract on Staphylococcus aureus Strains in Planktonic and Biofilm Growth Forms. Molecules. 2016;21:50.
Metadata
Title
Punica granatum peel extracts: HPLC fractionation and LC MS analysis to quest compounds having activity against multidrug resistant bacteria
Authors
Ilyas Khan
Hazir Rahman
Nasser M. Abd El-Salam
Abdul Tawab
Anwar Hussain
Taj Ali Khan
Usman Ali Khan
Muhammad Qasim
Muhammad Adnan
Azizullah Azizullah
Waheed Murad
Abdullah Jalal
Noor Muhammad
Riaz Ullah
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1766-4

Other articles of this Issue 1/2017

BMC Complementary Medicine and Therapies 1/2017 Go to the issue

Research article

Anti-stress and neuronal cell differentiation induction effects of Rosmarinus officinalis L. essential oil

Research article

Microscopic identification and in vitro activity of Agastache rugosa (Fisch. et Mey) from Xinjiang, China

Research article

Effects of phenolic constituents of daylily flowers on corticosterone- and glutamate-treated PC12 cells

Research article

Increase of glutathione, testosterone and antioxidant effects of Jurenia dolomiaea on CCl4 induced testicular toxicity in rat

Research article

Cryptotanshinone enhances the effect of Arsenic trioxide in treating liver cancer cell by inducing apoptosis through downregulating phosphorylated- STAT3 in vitro and in vivo

Research article

Clinical safety of combined therapy of immune checkpoint inhibitors and Viscum album L. therapy in patients with advanced or metastatic cancer