Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Neuroprotective effect of Aronia melanocarpa extract against glutamate-induced oxidative stress in HT22 cells

Authors: Hyeon Yong Lee, Jin Bae Weon, Gahee Ryu, Woo Seung Yang, Nam Young Kim, Myong Ki Kim, Choong Je Ma

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

Login to get access

Abstract

Background

Glutamate (an endogenous excitatory neurotransmitter) at high concentrations contributes to the development of neurodegenerative diseases. Aronia melanocarpa (A. melanocarpa) berries contain anthocyanins and have high antioxidant activities. In this study, we evaluated whether A. melanocarpa berries could protect neuronal cells against glutamate-induced oxidative stress.

Method

A. melanocarpa berries exerted a protective effect against cytotoxicity in HT22 mouse hippocampal cells by MTT assay. We evaluated oxidative stress parameters including ROS level, intracellular Ca2+ level, glutathione level and antioxidant enzyme activity in HT22 cells to elucidate the mechanism of its neuroprotective effect.

Results

A. melanocarpa berries decreased glutamate-induced death of HT22 cells. In addition, A. melanocarpa berries reduced ROS and intracellular Ca2+ levels. Glutathione level, antioxidant enzymes, glutathione reductase and glutathione peroxide activities and mitochondrial membrane potential were also increased in HT22 cells.

Conclusion

These results suggested that A. melanocarpa berries protected HT22 cells by exerting an antioxidant effect.
Literature
1.
Crapper DR, DeBoni U. Brain aging and Alzheimer’s disease. Can Psychiatric Assoc J. 1978;23(4):229–33.
2.
3.
Markesbery WR. Oxidative stress hypothesis in Alzheimer's disease. Free Radic Biol Med. 1997;23(1):134–47.CrossRefPubMed
4.
Fukui M, Song JH, Choi J, Choi HJ, Zhu BT. Mechanism of glutamate-induced neurotoxicity in HT22 mouse hippocampal cells. Eur J Pharmacol. 2009;617:1–11.CrossRefPubMed
5.
Hynd MR, Scott HL, Dodd PR. Glutamate-mediated excitotoxicity and neurodegeneration in Alzheimer’s disease. Neurochem Int. 2004;45(5):583–95.CrossRefPubMed
6.
Schubert D, Piasecki D. Oxidative glutamate toxicity can be a component of the excitotoxicity cascade. J Neurosci. 2001;21:7455–62.PubMed
7.
Choi DW. Glutamate neurotoxicity in cortical cell cultures is calcium dependent. Neurosci Lett. 1985;58:293–7.CrossRefPubMed
8.
Tan S, Wood M, Maher P. Oxidative stress induces a form of programmed cell death with characteristics of both apoptosis and necrosis in neuronal cells. J Neurochem. 1998;71:95–105.CrossRefPubMed
9.
Murphy TH, Miyamoto M, Sastre A, Schnaar RL, Coyle JT. Glutamate toxicity in neuronal cell line involves inhibition of cystine transport leading to oxidative stress. Neuron. 1989;2:1547–58.CrossRefPubMed
10.
Oszmiański J, Wojdylo A. Aronia melanocarpa Phenolics and their antioxidant activity. Eur Food Res Technol. 2005;221(6):809–13.CrossRef
11.
Ciocoiu M, Badescu L, Miron A, Badescu M. The involvement of a polyphenol-rich extract of black chokeberry in oxidative stress on experimental arterial hypertension. Evid Based Complement Alternat Med. 2013; doi:10.​1155/​2013/​912769.PubMedPubMedCentral
12.
Baum JI, Shouse SA, Gilbert W, Prior RL, Howard LR. The effect of black chokeberry (Aronia melanocarpa) on the prevention of obesity in C57BL/6J mice. FASEB J. 2013;27:861–4.
13.
Brzóska MM, Rogalska J, Galazyn-Sidorczuk M, Jurczuk M, Roszczenko A, Tomczyk M. Protective effect of Aronia melanocarpa polyphenols against cadmium-induced disorders in bone metabolism: a study in a rat model of lifetime human exposure to this heavy metal. Chem Biol Interact. 2015;229:132–46.CrossRefPubMed
14.
Natella F, Nardini M, Giannetti I, Dattilo C, Scaccini C. Coffee drinking influences plasma antioxidant capacity in humans. J Agric Food Chem. 2002;50(21):6211–6.CrossRefPubMed
15.
Serraino I, Dugo L, Dugo P, Mondello L, Mazzon E, Dugo G, Caputi AP, Cuzzocrea S. Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure. Life Sci. 2003;73(9):1097–114.CrossRefPubMed
16.
Weon JB, Lee B, Yun BR, Lee J, Ma CJ. Neuroprotective effects of 4, 5-dimethoxypyrocatechol isolated from Cynanchum paniculatum on HT22 cells. Pharmacog Mag. 2014;10(38):161–4.CrossRef
17.
Butterfield DA, Pocernich CB. The glutamatergic system and Alzheimer's disease: therapeutic implications. CNS Drugs. 2003;17(9):641–52.CrossRefPubMed
18.
Suzuki YS, Forman HJ, Sevanian A. Oxidants as stimulators of signal transduction. Free Radic Biol Med. 1997;22:269–85.CrossRefPubMed
19.
Albrecht P, Lewerenz J, Dittmer S, Noack R, Maher P, Methner A. Mechanisms of oxidative glutamate toxicity: the glutamate/cystine antiporter system xc- as a neuroprotective drug target. CNS Neurol Disord Drug Targets. 2010;3:373–82.CrossRef
20.
Kane DJ, Sarafian TA, Anton R, Hahn H, Gralla EB, Valentine JS, Ord T, Bredesen DE. Bcl-2 inhibition of neuronal death: decreased generation of reactive oxygen species. Science. 1993;262:1274–7.CrossRefPubMed
21.
Ankarcrona M, Dypbukt JM, Bonfoco E, Zhivotovsky B, Orrenius S, Lipton SA, Nicotera P. Glutamate-induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial function. Neuron. 1995;15(4):961–73.CrossRefPubMed
22.
Liu J, Li L, Suo WZ. HT22 hippocampal neuronal cell line possesses functional cholinergic properties. Life Sci. 2009;84:267–71.CrossRefPubMed
23.
Tan L, Yang HP, Pang W, Lu H, Hu YD, Li J, Lu SJ, Zhang WQ, Jiang YG. Cyanidin-3-O-galactoside and blueberry extracts supplementation improves spatial memory and regulates hippocampal ERK expression in senescence-accelerated mice. Biomed Environ Sci. 2014;27(3):186–96.PubMed
Metadata
Title
Neuroprotective effect of Aronia melanocarpa extract against glutamate-induced oxidative stress in HT22 cells
Authors
Hyeon Yong Lee
Jin Bae Weon
Gahee Ryu
Woo Seung Yang
Nam Young Kim
Myong Ki Kim
Choong Je Ma
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1716-1

Other articles of this Issue 1/2017

BMC Complementary Medicine and Therapies 1/2017 Go to the issue

Research article

Upper airway stabilization by osteopathic manipulation of the sphenopalatine ganglion versus sham manipulation in OSAS patients: a proof-of-concept, randomized, crossover, double-blind, controlled study

Study protocol

Study protocol on comparative effectiveness of mindfulness meditation and qigong on psychophysiological outcomes for patients with colorectal cancer: a randomized controlled trial

Research article

Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats

Research article

Prevalence, patterns, and perceived value of complementary and alternative medicine among HIV patients: a descriptive study

Research article

Protective effects of methanolic extract of Juglans regia L. leaf on streptozotocin-induced diabetic peripheral neuropathy in rats

Research article

Saikosaponins induced hepatotoxicity in mice via lipid metabolism dysregulation and oxidative stress: a proteomic study