Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

ACC 2024 Congress

Catch up on all the top stories and latest evidence in cardiology research with our ACC 2024 Congress hub page.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Neuroprotective and cognitive-enhancing effects of the combined extract of Cyperus rotundus and Zingiber officinale

Authors: Chatchada Sutalangka, Jintanaporn Wattanathorn

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

Login to get access

Abstract

Background

Currently, food supplements to improve age-related dementia are required. Therefore, we aimed to determine the effect of the combined extract of Cyperus rotundus and Zingiber officinale (CP1) on the improvement of age-related dementia in rats with AF64A-induced memory deficits.

Methods

Male Wistar rats weighing 180-200 g were orally given CP1 at doses of 100, 200 and 300 mg.kg-1 BW for a period of 14 days after bilateral intracerebroventricular administration of AF64A. Spatial memory was assessed in all rats every 7 days throughout the 14 day-experimental period. At the end of the study, neuronal density, acetylcholinesterase (AChE) activity, oxidative stress status and the activation of MAPK cascades in the hippocampus were determined.

Results

Enhanced memory, increased neuronal density, decreased AChE activity and decreased oxidative stress status together with activated pERK1/2 were observed in the hippocampus of CP1-treated rats. These results suggested that CP1 might improve memory via enhanced cholinergic function and decreased neurodegeneration and oxidative stress.

Conclusions

CP1 is a potential novel food supplement for dementia. However, further investigations on the subchronic toxicity of CP1 and drug interactions are required.
Appendix
Available only for authorised users
Literature
1.
World Health Organization and Alzheimer’s Disease International. Dementia: a public health priority. Geneva: WHO press; 2012.
2.
Lye TC, Piguet O, Grayson DA, Creasey H, Ridley LJ, Bennett HP, Broe GA. Hippocampal size and memory function in the ninth and tenth decades of life: The Sydney Older Persons Study. J Neurol Neurosurg Psychiatry. 2010;75:548–54.CrossRef
3.
Rusinek H, De Santi S, Frid D, Tsui WH, Tarshish CY, Convit A, et al. Regional brain atrophy rate predicts future cognitive decline: 6-Year longitudinal MR imaging study of normal aging. Radiology. 2003;229:691–6.CrossRefPubMed
4.
Ikarashi Y, Harigaya Y, Tomidokoro Y, Kanai M, Ikeda M, Matsubara E, et al. Decreased level of brain acetylcholine and memory disturbance in APPsw mice. Neurobiol Aging. 2004;25:483–90.CrossRefPubMed
5.
Schliebs R, Arendt T. The significance of the cholinergic system in the brain during aging and in Alzheimer’s disease. J Neural Transm. 2006;113:1625–44.CrossRefPubMed
6.
Bergmann I, Priestley JV, McMahon SB, Brocker EB, Toyka KV, Koltzenburg M. Analysis of cutaneous sensory neurons in transgenic mice lacking the low affinity neurotrophin receptor p75. Eur J Neurosci. 1997;9:18–28.CrossRefPubMed
7.
Mecocci P, Cherubini A, Polidori MC, Cecchetti R, Chionne F, Senin U. Oxidative stress and dementia: new perspectives in AD pathogenesis. Aging (Milano). 1997;9(4):51–2.
8.
Rungsanpanya T, Muangpaisan W, Praditsuwan R. Clinical practice with antidementia drugs in a geriatric clinic. J Medl Assoc Thai. 2012;98(8):1081–9.
9.
Kilani JS, Ghedira Z, Nasr N, Krifa M, Ghedira K, Franca DM, et al. Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus. Asian Pac J Trop Dis. 2014;7(2):105–12.CrossRef
10.
Kilani S, Ben SM, Limem I, Bouhlel I, Boubaker J, Bhouri W, Skandrani I, Neffatti A, Ben Ammar R, Dijoux FMG, Ghedira K, Chekir GL. In vitro evaluation of antibacterial, antioxidant, cytotoxic and apoptotic activities of the tubers infusion and extracts of Cyperus rotundus. Bioresour Technol. 2008;99(18):9004–8.
11.
Grzanna R, Lindmark L, Frondoza CG. Ginger—an herbal medicinal product with broad anti-inflammatory actions. J Med Food. 2005;8(2):125–32.CrossRefPubMed
12.
Sahdeo P, Amit KT. Ginger and Its Constituents: Role in Prevention and Treatment of Gastrointestinal Cancer. Gastroenterol Res Pract. 2015; doi:10.​1155/​2015/​142979
13.
Bashir A, Sultana B, Hassan AF, Munir A, Amjad M, Hassan Q. Investigation on the antioxidant activity of Dheela grass (Cyperus rotundus). Afr J Basic Appl Sci. 2012;4(1):1–6.
14.
Sharma R, Gupta R. Cyperus rotundus extract inhibits acetylcholinesterase activity from animal and plants as well as inhibits germination and seedling growth in wheat and tomato. Life Sci. 2007;80:2389–92.CrossRefPubMed
15.
Rabiei Z, Hojjati M, Rafieian-Kopaei M. Effect of Cyperus rotundus tubers ethanolic extract on learning and memory in animal model of Alzheimer. Biomed Aging Pathol. 2013;3(4):185–91.CrossRef
16.
Hemanth K, Tamatam A, Pal A, Khanum F. Neuroprotective effects of Cyperus rotundus on SIN-1 induced nitric oxide generation and protein nitration: ameliorative effect against apoptosis mediated neuronal cell damage. Neurotox. 2013;34:150–9.CrossRef
17.
Wattanathorn J, Jittiwat J, Tongun T, Muchimapura S, Ingkaninan K. Zingiber officinale mitigates brain damage and improves memory impairment in focal cerebral ischemic rat. Evid Based Complement Alternat Med. 2011; doi:10.​1155/​2011/​429505
18.
Saenghong N, Wattanathorn J, Muchimapura S, Tongun T, Piyavhatkul N, Banchonglikitkul C, Kajsongkram T. Zingiber officinale improves cognitive function of the middle-aged healthy women. Evid Based Complement Alternat Med. 2012; doi:10.​1155/​2012/​383062
19.
Yeh H, Chuang C, Chen H, Wan C, Chen T, Lin L. Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts. Food Sci Technol. 2014;55:329–34.
20.
Krishna S, Renu S. Isolation and identification of flavonoids from Cyperus Rotundus Linn. In vivo and in vitro. J drug deliv ther. 2013;3(2):109–13.
21.
Ancos B, Sgroppo S, Plaza L, Cano M. Possible nutritional and health-related value promotion in orange juice preserved by high-pressure treatment. J Sci Food Agric. 2002;82:790–6.CrossRef
22.
Benzie I, Strain J. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem. 1996;239:70–6.CrossRefPubMed
23.
George L, Ellman K, Valentino J. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharm. 1961;7:88–95.CrossRef
24.
Isomae K, Ishikawa M, Ohta M, Ogawa Y, Hasegawa H, Kohda T, Kamei J. Effects of T-82, a new quinoline derivative, on cholinesterase activity and extracellular acetylcholine concentration in rat brain. Jpn J Pharmacol. 2002;88:206–12.CrossRef
25.
Wattanathorn J, Muchimapura S, Thukham-Mee W, Ingkaninan K, Wittaya-Areekul S. Mangifera indica Fruit Extract Improves Memory Impairment, Cholinergic Dysfunction, and Oxidative Stress Damage in Animal Model of Mild Cognitive Impairment. Oxid Med Cell Longev. 2014; doi:10.​1155/​2014/​132097
26.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxide in animal tissues by thiobarbituricacid reaction. Anal Biochem. 1979;95:351–8.CrossRefPubMed
27.
McCord J, Fridovich I. Superoxide dismutase, an enzymic function for erythrocuprein (hemocuprein). J Biol Chem. 1969;244:6049–55.PubMed
28.
Goldblith S, Proctor B. Photometric determination of catalase activity. J Biol Chem. 1950;187:705–9.PubMed
29.
Eyer P, Podhradsky D. Evaluation of the micro method for determination of glutathione using enzymatic cycling and Ellman's reagent. Anal Biochem. 1986;153:57–66.CrossRefPubMed
30.
Gong Q, Pan L, Liu X, Wang Q, Huang H, Zhu Y. S propargyl-cysteine (ZYZ-802), a sulphur-containing amino acid, attenuates beta-amyloid-induced cognitive deficits and pro-inflammatory response: involvement of ERK1/2 and NF-jB pathway in rats. Amino Acids. 2011;40:601–10.CrossRefPubMed
31.
Kumar J. Herbal medicine for Type 2 diabetes. IJDDC. 2010;30:111–2.
32.
Sungkamanee S, Wattanathorn J, Muchimapura S, Thukham-mee W. Antiosteoporotic effect of combined extract of Morus alba and Polygonum odoratum. Oxid Med Cell Longev. 2014; doi:10.​1155/​2014/​579305
33.
Thiraphatthanavong P, Wattanathorn J, Muchimapura S, Thukham-mee W, Lertrat K, Suriharn B. The combined extract of purple waxy corn and ginger prevents cataractogenesis and retinopathy in streptozotocin-diabetic rats. Oxid Med Cell Longev. 2014; doi:10.​1155/​2014/​789406
34.
Thukham-Mee W, Wattanathorn J. Evaluation of safety and protective effect of combined extract of Cissampelos pareira and Anethum graveolens (PM52) against age-related cognitive impairment. Evid Based Complement Alternat Med. 2012; doi:10.​1155/​2012/​674101
35.
Kanti BP, Syed IR. Markers of oxidative stress in erythrocytes and plasma during aging in humans. Oxid Med Cell Longev. 2010;3(1):2–12.CrossRef
36.
37.
Lu TH, Hsieh SY, Yen CC, Wu HC, Chen KL, Hung DZ, et al. Involvement of oxidative stress-mediated ERK1/2 and p38 activation regulated mitochondria-dependent apoptotic signals in methylmercury-induced neuronal cell injury. Toxicol Lett. 2011;204(1):71–80.CrossRefPubMed
38.
39.
Daulatzai MA. Neurotoxic saboteurs: straws that break the hippo's (hippocampus) back drive cognitive impairment and Alzheimer's disease. Neurotox Res. 2013;24:407–59.CrossRefPubMed
40.
Lee YJ, Cho HN, Soh JW, Jhon GJ, Cho CK, et al. Oxidative stress-induced apoptosis is mediated by ERK1/2 phosphorylation. Exp Cell Res. 2003;291(1):251–66.CrossRefPubMed
41.
Riedel G, Platt B, Micheau J. Glutamate receptor function in learning and memory. Behav Brain Res. 2003;140:1–47.CrossRefPubMed
42.
Sutalangka C, Wattanathorn J, Muchimapura S, Thukham-mee W: Moringa oleifera mitigates memory impairment and neurodegeneration in animal model of age-related dementia. Oxid Med Cell Longev. 2013; doi:10.​1155/​2013/​695936
43.
Bitner RS, Bunnelle WH, Anderson DJ, Briggs CA, Buccafusco J, et al. Broad-spectrum efficacy across cognitive domains by alpha7 nicotinic acetylcholine receptor agonism correlates with activation of ERK1/2 and CREB phosphorylation pathways. J Neurosci. 2007;27(39):10578–87.CrossRefPubMed
44.
Alonso M, Medina JH, Pozzo-Miller L. ERK1/2 activation is necessary for BDNF to increase dendritic spine density in hippocampal CA1 pyramidal neurons. Learn Mem. 2004;11(2):172–8.CrossRefPubMedPubMedCentral
45.
Lee C, Park GH, Kim CY, Jang JH. [6]-Gingerol attenuates β-amyloid-induced oxidative cell death via fortifying cellular antioxidant defense system. Food Chem Toxicol. 2011;49(6):1261–9.CrossRefPubMed
46.
Pangpookiew P, Wattanathorn J, Muchimapura S, Thukhummee W. Quercetin-loaded zein based nanofiber patch: A novel cognitive enhancer. Int J Pharm Biomed Sci. 2012;3(3):103–8.
Metadata
Title
Neuroprotective and cognitive-enhancing effects of the combined extract of Cyperus rotundus and Zingiber officinale
Authors
Chatchada Sutalangka
Jintanaporn Wattanathorn
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1632-4

Other articles of this Issue 1/2017

BMC Complementary Medicine and Therapies 1/2017 Go to the issue

Research article

Korean mistletoe (Viscum album coloratum) extract regulates gene expression related to muscle atrophy and muscle hypertrophy

Research article

Vaccaria hypaphorine alleviates lipopolysaccharide-induced inflammation via inactivation of NFκB and ERK pathways in Raw 264.7 cells

Research article

The anti-cancer effects of Tualang honey in modulating breast carcinogenesis: an experimental animal study

Research article

The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation

Research article

Chemical composition, vasorelaxant, antioxidant and antiplatelet effects of essential oil of Artemisia campestris L. from Oriental Morocco

Erratum

Erratum to: The effect of pomegranate fresh juice versus pomegranate seed powder on metabolic indices, lipid profile, inflammatory biomarkers, and the histopathology of pancreatic islets of Langerhans in streptozotocin-nicotinamide induced type 2 diabetic Sprague–Dawley rats