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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Anti-arthritic property of crude extracts of Piptadeniastrum africanum (Mimosaceae) in complete Freund’s adjuvant-induced arthritis in rats

Authors: Marius Mbiantcha, Jabeen Almas, Simjee U. Shabana, Dastagir Nida, Faheem Aisha

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

Rheumatoid arthritis, disease of unknown causes is a rheumatic and autoimmune pathology, recognised for its increasing frequency and its adverse consequences. It is a disease that occurs in most cases between 50 and 60 years and women are more affected than men. This study aimed at evaluating immunomodulatory and anti-arthritis capacity of aqueous and methanol extracts of stem bark of Piptadeniastrum africanum (Mimosaceae).

Methods

ROS production from phagocytes, proliferation of T-cells, TNF-α and IL-1β production and cytotoxicity were performed by using chemiluminescence technique, liquid scintillation counter, ELISA and MTT assay, respectively. Anti-arthritic activity was evaluated using a model of adjuvant induced arthritis.

Results

Methanol and aqueous extracts of Piptadeniastrum africanum significantly (P < 0.001) inhibited extracellular and intracellular ROS production. These extracts also possess significant (P < 0.001) inhibitory activity on T-cell proliferation other than reduced TNF-α and IL-1β production. Piptadeniastrum africanum also significantly exhibited antiarthritic activity in complete Freund’s adjuvant induced arthritis in rat associated with a significant anti-inflammatory and anti-hyperalgesia activity.

Conclusions

Immunomodulatory, anti-inflammatory, antihyperalgesia and anti-arthritis potential revealed in this study approve that, Piptadeniastrum africanum is a plant rich in compounds with anti-arthritic properties.
Literature
1.
2.
Symmons DP, Barrett EM, Bankhead CR, Scott DG, Silman AJ. The incidence of rheumatoid arthritis in the United Kingdom: results from the Norfolk Arthritis Register. Br J Rheumatol. 1994;33(8):735–9.CrossRefPubMed
3.
NCCCC (National Collaborating Centre for Chronic Conditions). Rheumatoid arthritis: National clinical guideline for management and treatment in adults. London: Royal College of Physicians (UK); 2009. p. 275.
4.
Gibofsky A. Overview of epidemiology, pathophysiology, and diagnosis of rheumatoid arthritis. Am J Manag Care. 2012;18(13 Suppl):S295–302.PubMed
5.
Butler SH, Godefroy F, Besson JM, Weil-Fugazza J. A limited arthritic model for chronic pain studies in the rat. Pain. 1992;48:73–81.CrossRefPubMed
6.
Wang Q, Kuang H, Su Y, Sun Y, Feng J, Guo R, Chan K. Naturally derived antiinflammatory compounds from Chinese medicinal plants. J Ethnopharmacol. 2013;146(1):9–39.CrossRefPubMed
7.
Escandell JM, Recio MC, Máñez S, Giner RM, Cerdá-Nicolás M, Ríos JL. Dihydrocucurditacin B, isolated from cayaponiatayuya, reduces damage in adjuvant induced arthritis. Eur J Pharmacol. 2006;532:145–54.CrossRefPubMed
8.
Whidente GT, Boulet JM, Walker K. The role of central peripheral μ opioïd receptors ininflammatory pain and edema: a study using morphine and DiPOA. J Pharmacol ExpTher. 2005;314(3):1234–40.CrossRef
9.
Firestein GS, Xu WD, Townsend K, Broide D, Alvaro-Gracia J, Glasebrook A, Zvaifler NJ. Cytokines inchronic inflammatory arthritis. I. Failure to detect T cell lymphokines (interleukin 2 and interleukin 3) and presence of macrophage colony stimulating factor (CSF-1) and a novel mast cell growth factor in rheumatoid synovitis. J Exp Med. 1988;168(5):1573–86.CrossRefPubMed
10.
Firestein GS, Zvaifler NJ. How important are T cells in chronic rheumatoid synovitis?: II. T cell-independent mechanisms from beginning to end. Arthritis Rheum. 2002;46(2):298–308.CrossRefPubMed
11.
Bannwarth B, Bertin P, Binard A, Calvino B, Grilo RM, Saraux A, Sibilia J, Trèves R, Vergne-Salle P. Pain, inflammation and interaction nervous system/immune system. A Éditorial Paris, Institut UPSA de la douleur 2007; 135 pages.
12.
Holmberg J. Reduced ROS production triggers arthritis/The role of T cells in arthritis pathogenesis Medical Inflammation Research, Department of Experimental Medicine, Faculty of Medicine, Lund University 2004; 44pages.
13.
Hamdi H, Mariette X, Godot V, Weldingh K, Hamid AM, Prejean MV, Baron G, Lemann M, Puechal X, Breban M, Berenbaum F, Delchier JC, Flipo RM, Dautzenberg B, Salmon D, Humbert M, Emilie D. the RATIO (Recherche sur Anti-TNF et Infections Opportunistes) Study Group. Inhibition of anti-tuberculosis T-lymphocyte function with tumour necrosisfactor antagonists. Arthritis Res Ther. 2006;8(4):R114.CrossRefPubMedPubMedCentral
14.
Sibilia J, Sordet C. Le rituximab : une biothérapie originale dans les maladies autoimmunes. Rev Med Interne. 2005;26:485–500.CrossRefPubMed
15.
Feldmann M, Brennan FM, Maini RN. Role of cytokines in rheumatoid arthritis. Annu Rev Immunol. 1996;14:397–440.CrossRefPubMed
16.
Fairburn K, Stevens CR, Winyard PG, Kus M, Ward RJ, Cunningham J, et al. Oxidative stress and its control:apathogenetic role in inflammatory joint disease. Biochem Soc Trans. 1993;21(2):371–5.CrossRefPubMed
17.
Tak PP, Zvaifler NJ, Green DR, Firestein GS. Rheumatoid arthritis and p53: how oxidative stress might alter the course of inflammatory diseases. Immunol Today. 2000;21(2):78–2.CrossRefPubMed
18.
Bauerova K, Bezek S. Role of Reactive Oxygen and Nitrogen Species in Etiopathogenesis of Rheumatoid Arthritis. Gen Physiol Biophys 1999; 18 Spec No: 15–20.
19.
Pandey S. Various techniques for the evaluation of anti arthritic activity in animal models. J Adv Pharm Technol Res. 2010;1(2):164–71.PubMedPubMedCentral
20.
Bendele A. Animal models of rheumatoid arthritis. J Musculoskelet Neuronal Interact. 2001;4:377–85.
21.
Liu YL, Lin HM, Zou R, Wu JC, Han R, Raymond LN, Reid PF, Qin ZH. Suppression of complete Freund's adjuvant-induced adjuvant arthritis by cobratoxin. Acta Pharmacol Sin. 2009;30:219–27.CrossRefPubMedPubMedCentral
22.
Geetha T, Varalakshmi P. Anticomplement activity of triterpenes from Crataeva nurvala stem bark in adjuvant arthritis in rats. Gen Pharmacol. 1999;32:495–7.CrossRefPubMed
23.
Andersen ML, Santos EHR, Seabra MLV, Da Silva AAB, Tufik S. Evaluation of acute and chronic treatments with Harpagophytum procumbens on Freund’s adjuvant-induced arthritis in rats. J Ethnopharmacol. 2004;91:325–30.CrossRefPubMed
24.
Davis L, Kuttan G. Immunomodulatory activity of Withania somnifera. J Ethnopharmacol. 2000;71(1–2):193–200.CrossRefPubMed
25.
Bin-Hafeez B, Haque R, Parvez S, Pandey S, Sayeed I, Raisuddin S. Immunomodulatory effects of fenugreek (trigonella foenum graecum l.) extract in mice. Int Immunopharmacol. 2003;3(2):257–65.CrossRefPubMed
26.
Cheng JLW, You T, Hu C. Anti-inflammatory and immunomodulatory activities of the extracts from the inflorescence of Chrysanthemum indicum Linné. J Ethnopharmacol. 2005;101(1–3):334–7.CrossRefPubMed
27.
Jadhav H, Singh A, Bhutani K. Rationale for immunomodulatory and anti-inammatory effects of Ocimum sanctum: radical scavenging potential and effect on nitric oxide production. Acta Hort. 2005;678(4):159–62.CrossRef
28.
Jiofack RB. Piptadeniastrum africanum (Hook). Brenan. In: Louppe D, Oteng-Amoako AA, Brink M, editors. Prota 7 (1): Timbers/Bois d'oeuvre 1. Wageningen: PROTA; 2008.
29.
Noumi E, Yomi A. Medicinal plants used for intestinal diseases in Mbalmayo Region, Central Province, Cameroon. Fitoterapia. 2001;72:246–50.CrossRefPubMed
30.
Betti JL. Medicinal plants sold in Yaounde markets. Cameroon Afr Study Monogr. 2002;23(2):47–64.
31.
Sumitra S, Nidhi S. Evaluation of in vitro anti arthritic activity of Acacia auriculiformis A. Cunn. Ex. Benth. stem bark. World J Pharm Pharm Sci. 2016;5(2):1659–64.
32.
Samrat C, Lalit K, Navpreet K, Randhir S. Potential Anti-Arthritic Agents From Indian Medicinal Plants. Research and Reviews. J Pharm Pharm Sci. 2015;4(3):10–22.
33.
Pathak N, Gohil P, Patel NB, Kasture S, Jivani N, Bhalodia Y. Curative Effect of Albizia lebbeck Methanolic Extract against Adjuvant Arthritis-With Special Reference to Bone Erosion. Int J Pharm Sci Drug Res. 2009;1(3):183–7.
34.
Mengome LE, Feuya TGR, Eba F, Nsi-Emvo E. Antiproliferative Effect of Alcoholic Extracts of Some Gabonese Medicinal Plants on Human Colonic Cancer Cells. Afr J Tradit Complement Altern Med. 2009;6(2):112–7.
35.
Assob JCN, Kamga HLF, Nsagha DS, Njunda AL, Nde PF, Asongalem EA, Njouendou AJ, Sandjon B, Penlap VB. Antimicrobial and toxicological activities of five medicinal plant species from Cameroon Traditional Medicine. BMC Complement Altern Med. 2011;11:70.CrossRefPubMedPubMedCentral
36.
Ateufack G, Domgnim MCE, Mbiantcha M, Dongmo FBR, Nana D, Kamanyi A. Gastroprotective and ulcer healing effects of piptadeniastrum Africanum on experimentally induced gastric ulcers in rats. BMC Complement Altern Med. 2015;15:214.CrossRefPubMedPubMedCentral
37.
Diffoum JB. Propriétés analgésiques et anti-inflammatoires de l’extrait aqueux des écorces de Piptadeniastrum africanum (Mimosaceae) chez le rat. LAPHYPHA, UDs: Thèse de MASTER 2012; 94pages
38.
Note OP, Tapondjou AL, Mitaine-offer AC. iyamoto TM, Pegnyemb DE, Lacaille-Dubois MA. Triterpenoid saponins from Piptadeniastrum africanum (Hook. f.) Brenan. Phytochem Lett. 2013;6(4):505–10.CrossRef
39.
Chandrasenan P, Neethu MV, Anjumol VM, Anandan V, Selvaraj R. Triterpenoid fraction isolated from Euphorbia tirucalli Linn. ameliorates collagen induced arthritis in Wistar rats. J App Pharm Sci. 2016;6(1):070–5.CrossRef
40.
Almas J, Mesaik MA, Shabana US, Lubna SB, Shaheen F. Anti-TNF-α and anti-arthritis of patuletin: A rare flavonoid from Tagetes patula. Int Immunopharmacol. 2016;36:232–40.CrossRef
41.
Rita C, Bruno V, Helena R, Ana NC, Nuno F, Vineet G, João EF, Luis FM. Potent Anti-Inflammatory and Antiproliferative Effects of Gambogic Acid in a Rat Model of Antigen-Induced Arthritis. Mediators of Inflammation 2014; 7pages
42.
Zimmerman M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983;16(2):109–10.CrossRef
43.
Mesaik MA, Ul-Haq Z, Murad S, Ismail Z, Abdullah NR, Gill HK, Yousaf M, Siddiqui RA, Ahmad A, Choudhary MI. Biological and molecular docking studies on coagulin-H: Human IL-2 novel natural inhibitor. Mol Immunol. 2006;43(11):1855–163.CrossRefPubMed
44.
Mahomoodally MF, Gurib-Fakim A, Subratty AH. Effect of exogenous ATP on Momordica charantia Linn. (Cucurbitaceae) induced inhibition of D-glucose, L-tyrosine and fluid transport across rat everted intestinal sacs in vitro. J Ethnopharmacol. 2007;110(2):257–63.CrossRefPubMed
45.
Singh U, James T, Senthil KV, Sridevi D, Ishwarlal J. Development of an In Vitro Screening Assay to Test the Anti-inflammatory Properties of Dietary Supplements and Pharmacologic Agents. Clin Chem. 2005;51(12):2252–6.CrossRefPubMed
46.
Scholz G, Pohl I, Genschow E, Klemm M, Spielmann H. Embryotoxicity Screening Using Embryonic Stem Cells in vitro: Correlation to in vivo Teratogenicity. Cells Tissues Organs. 1999;165:203–11.CrossRefPubMed
47.
Taniguchi N, Kanai S, Kawamoto M, Endo H, Higashino H. Study on application of static magnetic field for adjuvant arthritis rats. Evid Based Complement Altern Med. 2004;1:187–91.CrossRef
48.
Winter CA, Risley EA, Nuss CW. Carageenan induced edema in hind paw of the rats as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med. 1962;111:544–7.CrossRefPubMed
49.
Bihani GV, Rojatkar SR, Bodhankar SL. Anti-arthritic activity of methanol extract of Cyathocline purpurea (whole plant) in Freund’s complete adjuvant-induced arthritis in rats. Biomedicine & Aging Pathology. 2014;4:197–206.CrossRef
50.
Randall LO, Selitto JJ. A method for measurement of analgesic activity on inflamed tissue. Arch Int Pharmacodyn Ther. 1957;111:409–19.PubMed
51.
Hargreaves K, Dubner R, Brown F, Flores C, Joris J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain. 1988;32:77–88.CrossRefPubMed
52.
Gabriella C, Maristella A, Elena G, Iwan JP. de Esch, Rob L. Antiinflammatory and antinociceptive effects of the selective histamine H4-receptor antagonists JNJ7777120 and VUF6002 in a rat model of carrageenan-induced acute inflammation. Eur J Pharmacol. 2007;563:240–4.CrossRef
53.
Foyet HS, Tsala DE, Zogo Essono Bodo JC, Carine AN, Heroyne LT, Oben EK. Anti-inflammatory and antiarthritic activity of a methanol extract from Vitellaria paradoxa stem bark. Phcog Res. 2015;7:367–77.CrossRefPubMedCentral
54.
Mythilypriya R, Shanthi P, Sachdanandam P. Salubrious effect of Kalpaamruthaa, a modified indigenous preparation in adjuvant-induced arthritis in rats – A biochemical approach. Chem Biol Interact. 2008;173:148–58.CrossRefPubMed
55.
Mehta A, Sethiya N, Mehta C, Shah G. Anti-arthritis activity of roots of Hemidesmus indicus R. Br. (Anantmul) in rats. Asian Pac J Trop Med. 2012;5(2):130–5.CrossRefPubMed
56.
Patil K, Suryavanshi J. Effect of Celastrus paniculatus Willd. seed on adjuvant induced arthritis in rats. Pharmacogn Mag. 2007;3(11):177–81.
57.
58.
Rhee DK, Marcelino J, Baker M, et al. The secreted glycoprotein lubricin protects cartilage surfaces and inhibits synovial cell overgrowth. J Clin Invest. 2005;115:622–31.CrossRefPubMedPubMedCentral
59.
van der Heijde DM. Joint erosions and patients with early rheumatoid arthritis. Br J Rheumatol. 1995;34 Suppl 2:74–8.CrossRefPubMed
60.
Visser H, le Cessie S, Vos K, Breedveld FC, Hazes JM. How to diagnose rheumatoid arthritis early: a prediction model for persistent (erosive) arthritis. Arthritis Rheum. 2002;46:357–65.CrossRefPubMed
61.
Gravallese EM, Harada Y, Wang JT, Gorn AH, Thornhill TS, Goldring SR. Identification of cell types responsible for bone resorption in rheumatoid arthritis and juvenile rheumatoid arthritis. Am J Pathol. 1998;152(4):943–51.PubMedPubMedCentral
62.
Schett G, Teitelbaum SL. Osteoclasts and arthritis. J Bone Miner Res. 2009;24:1142–6.CrossRef
63.
64.
Hess A, Axmann R, Rech J, Finzel S, Heindl C, Kreitz S, Sergeeva M, Saake M, Garcia M, Kollias G, Straub RH, Sporns O, Doerfler A, Brune K, Schett G. Blockade of TNF-α rapidly inhibits pain responses in the central nervous system. Proc Natl Acad Sci U S A. 2011;108(9):3731–6.CrossRefPubMedPubMedCentral
65.
Calamia KT. Current and future use of anti-TNF agents in the treatment of autoimmune, inflammatory disorders. Adv Exp Med Biol. 2003;528:545–9.CrossRefPubMed
66.
Akira S, Hirano T, Taga T, Kishimoto T. Biology of multifunctional cytokines: IL-6 and related molecules (IL 1 and TNF). FASEB J. 1990;4(11):2860–7.PubMed
67.
Almas J. Study of the suppression of inflammatory arthritis at molecular level by natural and synthetic inhibition of TNFα and IL-1β. Ph. D thesis 2013; 190 pages
68.
Dawé A, Mbiantcha M, Fongang Y, Nana WY, Yakai F, Ateufack G, Shaiq MA, Lubna I, Lateef M, Ngadjui BT. Piptadenin, a Novel 3,4-secooleanane Triterpene and Piptadenamide, a New Ceramide from the stem bark of Piptadeniastrum africana (Hook. f.) Brenan. Chem Biodiversity 2016; Accepted Author Manuscript. doi:10.​1002/​cbdv.​201600215
69.
Rabi T, Shukla S, Gupta S. Betulinic Acid Suppresses Constitutive and TNFα-induced NF-κB Activation and Induces Apoptosis in Human Prostate Carcinoma PC-3 Cells. Mol Carcinog. 2008;47(12):964–73.CrossRefPubMedPubMedCentral
70.
Yu-Jin H, Jaewhan S, Haeng-Ran K, Kyung-A H. Oleanolic acid regulates NF-κB signaling by suppressing MafK expression in RAW 264.7 cells. BMB Rep. 2014;47(9):524–9.CrossRef
71.
Silva JR L e, da Silva MD P, Lefort J, Vargaftig BB. Endotoxins, asthma, and allergic immune responses. Toxicology. 2000;152(1–3):31–5.CrossRef
72.
Harris SG, Padilla J, Koumas L, Ray D, Phipps RP. Prostaglandins as modulators of immunity. Trends Immunol. 2002;23(3):144–50.CrossRefPubMed
73.
Dudhgaonkar S, Thyagarajan A, Sliva D. Suppression of the inflammatory response by triterpenes isolated from the mushroom Ganoderma lucidum. Int Immunopharmacol. 2009;9(11):1272–80.CrossRefPubMed
74.
Behm BW, Bickston SJ. Tumor necrosis factor-alpha antibody for maintenance of remission in Crohn's disease. Cochrane Database Syst Rev. 2008, Issue 1. Art. No.: CD006893. doi: 10.​1002/​14651858.​CD006893 .
75.
Clemedson C, Ekwall B. Overview of the final MEIC results. I. The in vitro–in vitro evaluation. Toxicol In Vitro. 1999;13:657–63.CrossRefPubMed
76.
Scheers ME, Ba E, Dierickx JP. In vitro long-term cytotoxicity testing of 27 MEIC chemicals on HepG2 cells and comparison with acute human toxicity data. Toxicol In Vitro. 2001;15(2):153–61.CrossRefPubMed
77.
Weyermann J, Lochmann D, Zimmer A. A practical note on the use of cytotoxicity assays. Int J Pharmaceut. 2005;288(2):369–76.CrossRef
78.
Delongeas JL, Burnel D, Netter P, Grignon M, Mur JM, Royer R, Grignon G. Toxicity and pharmacokinetics of zirconium oxychloride in mice and rats. J Pharmacol. 1983;14(4):437–47.PubMed
79.
Lu FC. General data, Evaluation Procedures, target organs and assessment of risk. Toxicology Masson Paris 1992; 361pages.
80.
Benslay DN, Bendele AM. Development of a rapid screen for detecting and differentiating immunomodulatory vs anti-inflammatory compounds in rats. Agents Actions. 1991;34:254–6.CrossRefPubMed
81.
Colpaert FC, Meert T, Witte P, Schmitt P. Further evidence validating adjuvant arthritis as an experimental model of chronic pain in the rat. Life Sci. 1982;31:67–75.CrossRefPubMed
82.
Singh S, Majumdar DK. Effect of fixed oil of Ocimum sanctum against experimentally induced arthritis and joint edema in laboratory animals. Int J Pharmacogn. 1996;34:218–22.CrossRef
83.
Higgins M, D’Agostino R, Kannel W, Cobb J. Benefits and adverse effects of weight loss: observations from the Framingham study. Ann Intern Med. 1993;119:758–63.CrossRefPubMed
84.
85.
Fang JF, Liang Y, Du JY, Fang JQ. Transcutaneous electrical nerve stimulation attenuates CFA-induced hyperalgesia and inhibits spinal ERK1/2-COX-2 pathway activation in rats. BMC Complement Altern Med. 2013;13:134.CrossRefPubMedPubMedCentral
86.
Milovanoic M, Nilson E, Jaremo P. Relationship between platelets and inflammatory markers in rheumatoid arthritis. Clin Chim Acta J. 2004;343:237–40.CrossRef
87.
88.
Yildirim K, Karatay S, Melikoglu MA, Gureser G, Ugur M, Senel K. Associations between acute phase reactant levels and disease activity score (DAS28) in patient with rheumatoid arthritis. Ann Clin Lab Sci. 2004;34(4):423–6.PubMed
89.
Rainsford KD. Adjuvant polyarthritis in rats. Is this a satisfactory model for screening anti-arthritic drugs? Agents Actions. 1982;12:452–8.CrossRefPubMed
90.
Glenn EM, Gray J, Kooyers W. Chemical changes in adjuvant induced polyarthritis of rats. Am J Vet Res. 1965;26(114):1195–203.PubMed
91.
Niino-Nanke Y, Akama H, Hara M, Kashiwazaki S. Alkaline phasphatase (ALP) activity in rheumatoid arthritis-its clinical significance and synthesis of ALP in RA synovium. Ryumachi. 1998;38(4):581–8.PubMed
92.
93.
94.
Harris ED. Rheumatoid arthritis: pathophysiology and implications for therapy. N Engl J Med. 1990;322:1277–89.CrossRefPubMed
Metadata
Title
Anti-arthritic property of crude extracts of Piptadeniastrum africanum (Mimosaceae) in complete Freund’s adjuvant-induced arthritis in rats
Authors
Marius Mbiantcha
Jabeen Almas
Simjee U. Shabana
Dastagir Nida
Faheem Aisha
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1623-5

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