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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

In vitro α-glucosidase inhibitory activity of isolated fractions from water extract of Qingzhuan dark tea

Authors: Shuyuan Liu, Zhi Yu, Hongkai Zhu, Wei Zhang, Yuqiong Chen

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

Natural products have being used as potential inhibitors against carbohydrate-hydrolyzing enzymes to treat diabetes mellitus. Chinese dark tea has various interesting bioactivities. In this study, the active compounds from Qingzhuan dark tea were separated and their anti-diabetic activity was examined using an in vitro enzymatic model.

Methods

The chloroform, ethyl acetate, n-butanol, sediment and residual aqua fractions of a Chinese dark tea (Qingzhuan tea) were prepared by successively isolating the water extract with different solvents and their in vitro inhibitory activities against α-glucosidase were assessed. The fraction with the highest inhibitory activity was further characterized to obtain the main active components of Qingzhuan tea.

Results

The ethyl acetate fraction had the greatest inhibitory effect on α-glucosidase, followed by n-butanol, sediment and residual aqua fractions (with the IC50 values of 0.26 mg/mL, 2.94 mg/mL, 3.02 mg/mL, and 5.24 mg/mL, respectively), mainly due to the high content of polyphenols. Among the eight subfractions (QEF1-8) isolated from the ethyl acetate fraction, QEF8 fraction showed the highest α-glucosidase inhibitory potential in a competitive inhibitory manner (the K i value of 77.10 μg/mL). HPLC-MS analysis revealed that (−)-epigallocatechin gallate (EGCG) and (−)-epicatechin gallate (ECG) were the predominant active components in QEF8.

Conclusion

These results indicated that Qingzhuan tea extracts exerted potent inhibitory effects against α-glucosidase, EGCG and ECG were likely responsible for the inhibitory activity in Qingzhuan tea. Qingzhuan tea may be recommended as an oral antidiabetic diet.
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Metadata
Title
In vitro α-glucosidase inhibitory activity of isolated fractions from water extract of Qingzhuan dark tea
Authors
Shuyuan Liu
Zhi Yu
Hongkai Zhu
Wei Zhang
Yuqiong Chen
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1361-0

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