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BMC Complementary Medicine and Therapies

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Open Access 01-12-2016 | Research article

The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells

Authors: Qi Zhang, Zi-Jian Zhang, Xing-Hua Wang, Jie Ma, Yue-Han Song, Mi Liang, Sen-Xiang Lin, Jie Zhao, Ao-Zhe Zhang, Feng Li, Qian Hua

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Abstract

Background

Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of “tonifying qi and strengthening spleen” group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and “eliminating phlegm and strengthening intelligence” group (EPSI) composed of Polygala tenuifolia Willd., Acorus calamus L. and Sinapis alba L from the herb complex on neurite growth in PC12 cells, two disassembled prescriptions derived from Shenghui soup and their molecular mechanisms were investigated.

Methods

Firstly, CCK-8 kit was used to detect the impact of the two prescriptions on PC12 cell viability; and Flow cytometry was performed to measure the cell apoptosis when PC12 cells were treated with these drugs. Secondly, the effect of the two prescriptions on the differentiation of PC12 cells was observed. Finally, the mRNA and protein expression levels of GAP-43 were analyzed by RT-PCR and western blot, respectively.

Results

“Tonifying qi and strengthening spleen” prescription decreased cell viability in a dose-dependent manner, but had no significant effect on cell apoptosis. Meanwhile, it could improve neurite growth and elevate the mRNA and protein expression level of GAP-43. “Eliminating phlegm and strengthening intelligence” prescription also exerted the similar effects on cell viability and apoptosis. Furthermore, it could also enhance cell neurite growth, with a higher expression level of GAP-43 mRNA and protein.

Conclusion

“Tonifying qi and strengthening spleen” and “eliminating phlegm and strengthening intelligence” prescriptions from Shenghui soup have a positive effect on neurite growth. Their effects are related to the up-regulating expression of GAP-43.

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Title: The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells
Authors: Qi Zhang
Zi-Jian Zhang
Xing-Hua Wang
Jie Ma
Yue-Han Song
Mi Liang
Sen-Xiang Lin
Jie Zhao
Ao-Zhe Zhang
Feng Li
Qian Hua
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-016-1339-y

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

The prescriptions from Shenghui soup enhanced neurite growth and GAP-43 expression level in PC12 cells

Abstract

Background

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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