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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae)

Authors: Manoela Torres-Rêgo, Allanny Alves Furtado, Mariana Angélica Oliveira Bitencourt, Maira Conceição Jerônimo de Souza Lima, Rafael Caetano Lisbôa Castro de Andrade, Eduardo Pereira de Azevedo, Thaciane da Cunha Soares, José Carlos Tomaz, Norberto Peporine Lopes, Arnóbio Antônio da Silva-Júnior, Silvana Maria Zucolotto, Matheus de Freitas Fernandes-Pedrosa

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

Hancornia speciosa Gomes (Apocynaceae), popularly known as “mangabeira,” has been used in folk medicine to treat inflammatory disorders, hypertension, dermatitis, diabetes, liver diseases and gastric disorders. Although the ethnobotany indicates that its fruits can be used for the treatment of ulcers and inflammatory disorders, only few studies have been conducted to prove such biological activities. This study investigated the anti-inflammatory properties of the aqueous extract of the fruits of H. speciosa Gomes as well as its bioactive compounds using in vivo experimental models.

Methods

The bioactive compounds were identified by High Performance Liquid Chromatography coupled with diode array detector (HPLC-DAD) and Liquid Chromatography coupled with Mass Spectrometry (LC-MS). The anti-inflammatory properties were investigated through in vivo tests, which comprised xylene-induced ear edema, carrageenan-induced peritonitis and zymosan-induced air pouch. The levels of IL-1β, IL-6, IL-12 and TNF-α were determined using ELISA.

Results

Rutin and chlorogenic acid were identified in the extract as the main secondary metabolites. In addition, the extract as well as rutin and chlorogenic acid significantly inhibited the xilol-induced ear edema and also reduced the cell migration in both carrageenan-induced peritonitis and zymosan-induced air pouch models. Reduced levels of cytokines were also observed.

Conclusion

This is the first study that demonstrated the anti-inflammatory activity of the extract of H. speciosa fruits against different inflammatory agents in animal models, suggesting that its bioactive molecules, especially rutin and chlorogenic acid are, at least in part, responsible for such activity. These findings support the widespread use of Hancornia speciosa in popular medicine and demonstrate that its aqueous extract has therapeutical potential for the development of herbal drugs with anti-inflammatory properties.
Literature
1.
Luiz-Ferreira A, Cola-Miranda M, Barbastefano V, Hiruma-Lima CA, Vilegas W, Souza Brito ARM. Should Anacardium humile St. Hil be used as an antiulcer agent? A scientific approach to the traditional knowledge. Fitoterapia. 2008;79:207–9.CrossRefPubMed
2.
Yaseen G, Ahmad M, Sultana S, Suleiman Alharrasi A, Hussain J, Zafar M, Shafiq-Ur-Rehman. Ethnobotany of Medicinal Plants in the Thar Desert (Sindh) of Pakistan. J Ethnopharmacol. 2015;163:43–59.
3.
Calixto JB. Twenty-five years of research on medicinal plants in Latin America. A personal view. J Ethnopharmacol. 2005;100:131–4.CrossRefPubMed
4.
5.
Rodrigues VEG, Carvalho DA. Levantamento Etnobotânico De Plantas Medicinais No Domínio do Cerrado Na Região Do Alto Etnobotanical. Ciênc Agrotec. 2001;25:102–23.
6.
Sampaio TS. Estudo Fitoquímico de Hancornia speciosa Gomes: Isolamento, Determinação Estrutural e Atividade Biológica. 190f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, Sergipe;2008.
7.
Almeida SP, Proença CEB, Sano SM, Ribeiro JF. Cerrado: Espécies vegetais úteis. EMBRAPA, Planaltina;1998:38.
8.
Grandi TMS, Lima-Filho FM, Ferreira SMA. Levantamento das plantas medicinais de Grão o Mogol. Oréades. 1982;8:116–25.
9.
Hirschmann GS, Arias AR. A Survey of medicinal plants of Minas Gerais, Brazil. J Ethnopharmacol. 1990;29:159–72.CrossRefPubMed
10.
Ferreira HC, Serra CP, Lemos VS, Braga FC, Cortes SF. Nitric oxide-dependent vasodilatation by ethanolic extract of Hancornia speciosa via phosphatidyl-inositol 3-kinase. J Ethnopharmacol. 2007;109:161–4.CrossRefPubMed
11.
Ferreira HC, Serra CP, Endringer DC, Lemos VS, Braga FC, Cortes SF. Endothelium-dependent vasodilation induced by Hancornia speciosa in rat superior mesenteric artery. Phytomedicine. 2007;14:473–8.CrossRefPubMed
12.
Silva GC, Braga FC, Lima MP, Pesquero JL, Lemos VS, Cortes SF. Hancornia speciosa Gomes induces hypotensive effect through inhibition of ACE and increase on NO. J Ethnopharmacol. 2011;137:709–13.CrossRefPubMed
13.
Endringer DC, Pezzuto JM, Braga FC. NF-kappaB inhibitory activity of cyclitols isolated from Hancornia speciosa. Phytomedicine. 2009;16:1064–9.CrossRefPubMed
14.
Endringer DC, Valadares YM, Campana PRV, Campos JJ, Guimarães KG, Pezzuto JM, Braga FC. Evaluation of Brazilian Plants on Cancer Chemoprevention Targets In Vitro. Phyther Res. 2010;24:928–33.
15.
Pereira AC, Pereira ABD, Moreira CCL, Botion LM, Lemos VS, Braga FC, Cortes SF. Hancornia speciosa Gomes (Apocynaceae) as a potential anti-diabetic drug. J Ethnopharmacol. 2015;161:30–5.CrossRefPubMed
16.
Moraes TDM, Rodrigues CM, Kushima H, Bauab TM, Villegas W, Pellizzon CH, Brito ARMS, Hiruma-Lima CA. Hancornia speciosa: indications of gastroprotective, healing and anti-Helicobacter pylori actions. J Ethnopharmacol. 2008;120:161–8.CrossRef
17.
Rodrigues CM, Rinaldo D, Santos LC, Montoro P, Hiruma-Lima A, Brito ARMS, Piacente S, Pizza C, Vilegas W. Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa. Rapid Commun Mass Spectrom. 2007;21:1907–14.CrossRefPubMed
18.
Lima JP, Azevedo L, de Souza NJ, Nunes EE, Vilas Boas EVDB. First evaluation of the antimutagenic effect of mangaba fruit in vivo and its phenolic profile identification. Food Res Int. 2015;75:216–24.CrossRef
19.
Marinho DG, Alviano DS, Matheus ME, Alviano CS, Fernandes PD. The latex obtained from Hancornia speciosa Gomes possesses anti-inflammatory activity. J Ethnopharmacol. 2011;135:530–7.CrossRefPubMed
20.
Carvalho W, Lemônica L. Artigo de Revisão Mecanismos Celulares e Moleculares da Dor Inflamatória. Modulação Periférica e Avanços Terapêuticos. Rev Bras Anestesiol. 1998;48:137–58.
21.
22.
Tayal V, Kalra BS. Cytokines and anti-cytokines as therapeutics - An update. Eur J Pharmacol. 2008;579:1–12.CrossRefPubMed
23.
24.
Sostres C, Gargallo CJ, Arroyo MT, Lanas A. Adverse effects of non-steroidal anti-inflammatory drugs (NSAIDs, aspirin and coxibs) on upper gastrointestinal tract. Best Pract Res Clin Gastroenterol. 2010;24:121–32.CrossRefPubMed
25.
Whitehouse MW. Anti-inflammatory glucocorticoid drugs: Reflections after 60 years. Inflammopharmacology. 2011;19:1–19.CrossRefPubMed
26.
27.
Saklani A, Kutty SK. Plant-derived compounds in clinical trials. Drug Discov Today. 2008;13:161–71.CrossRefPubMed
28.
Itokawa H, Morris-Natschke SL, Akiyama T, Lee KH. Plant-derived natural product research aimed at new drug discovery. J Nat Med. 2008;62:263–80.CrossRefPubMed
29.
Kolewe ME, Gaurav V, Roberts SC. Pharmaceutically active natural product synthesis and supply via plant cell culture technology. Mol Pharm. 2008;5:243–56.CrossRefPubMed
30.
Kolaczkowska E, Koziol A, Plytycz B, Arnold B. Inflammatory macrophages, and not only neutrophils, die by apoptosis during acute peritonitis. Immunobiology. 2010;215:492–504.CrossRefPubMed
31.
Magalhães A, Santos GB, Verdam MCDS, Fraporti L, Malheiro A, Lima ES, Dos-Santos MC. Inhibition of the inflammatory and coagulant action of Bothrops atrox venom by the plant species Marsypianthes chamaedrys. J Ethnopharmacol. 2011;134:82–8.CrossRefPubMed
32.
Cunha FQ, Souza GEP, Souza CAM, Cerqueira BCS, Ferreira SH. In vivo blockage of neutrophil migration by LPS is mimicked by a factor released from LPS-stimulated macrophages. Br J Exp Pathol. 1989;70:1–8.PubMedPubMedCentral
33.
Parveen Z, Deng Y, Saeed MK, Dai R, Ahamad W, Yu Y. Antiinflammatory and Analgesic Activities of Thesium chinense Turcz Extracts and its Major Flavonoids, Kaempferol and Kaempferol-3-O-glucoside. Pharm Soc Japan. 2007;127:1275–9.
34.
Pinheiro MMG, Miltojević AB, Radulović NS, Abdul-Wahab IR, Boylan F, Fernandes PD. Anti-Inflammatory Activity of Choisya ternata Kunth Essential Oil, Ternanthranin, and Its Two Synthetic Analogs (Methyl and Propyl N-Methylanthranilates). PLoS One. 2015;10:e0121063.CrossRefPubMedPubMedCentral
35.
Vigil SVG, de Liz R, Medeiros YS, Fröde TS. Efficacy of tacrolimus in inhibiting inflammation caused by carrageenan in a murine model of air pouch. Transpl Immunol. 2008;19:25–9.CrossRefPubMed
36.
Yoon SY, Kwon YB, Kim HW, Roh DH, Kang SY, Kim CY, Han HJ, Kim KW, Yang IS, Beitz AJ, Lee JH. Intrathecal neostigmine reduces the zymosan-induced inflammatory response in a mouse air pouch model via adrenomedullary activity: involvement of spinal muscarinic type 2 receptors. Neuropharmacology. 2005;49:275–82.CrossRefPubMed
37.
Fialho EMS, Maciel MCG, Silva ACB, Reis AS, Karine A, Assunção M, Fortes TS, Silva LA, Guerra RNM, Kwasniewski FH, Nascimento FRF. Immune cells recruitment and activation by Tityus serrulatus scorpion venom. Toxicon. 2011;58:480–5.CrossRefPubMed
38.
39.
Gomes EB, Ramanho SR, Gualberto NC, Miranda RCM, Nigam N, Norain N. A Rapid Method for Determination of Some Phenolic Acids in Brazilian Tropical Fruits of Mangaba (Hancornia speciosa Gomes) and Umbu (Spondias tuberosa Arruda Camara) by UPLC. Journal of Analytical Sciences, Methods and Instrumentation. 2013;3:1–10.CrossRef
40.
Lima MCJDS, Bitencourt MAO, Furtado AA, Oliveira Rocha HA, Oliveira RM, Silva-Júnior AA, Tabosa Do Egito ES, Tambourgi DV, Zucolotto SM, Fernandes-Pedrosa MDF. Ipomoea asarifolia neutralizes inflammation induced by Tityus serrulatus scorpion venom. J Ethnopharmacol. 2014;153:890–5.CrossRefPubMed
41.
Xu Y, Chen J, Yu X, Tao W, Jiang F, Yin Z, Liu C. Protective effects of chlorogenic acid on acute hepatotoxicity induced by lipopolysaccharide in mice. Inflamm Res. 2010;59:871–7.CrossRefPubMed
42.
Zhang X, Huang H, Yang T, Ye Y, Shan J, Yin Z, Luo L. Chlorogenic acid protects mice against lipopolysaccharide-induced acute lung injury. Injury. 2010;41:746–52.CrossRefPubMed
43.
Ribeiro TP, Sousa TR, Arruda AS, Peixoto N, Gonçalves PJ, Almeida LM. Evaluation of cytotoxicity and genotoxicity of Hancornia speciosa latex in Allium cepa root model. Braz J of Biol. 2016;76:1–5.CrossRef
44.
Syam S, Bustamam A, Abdullah R, Sukari MA, Hashim NM, Mohan S, Looi CY, Wong WF, Yahayu MA, Abdelwahab SI. β Mangostin suppress LPS-induced inflammatory response in RAW 264.7 macrophages in vitro and carrageenan-induced peritonitis in vivo. J Ethnopharmacol. 2014;153:435–45.CrossRefPubMed
45.
Vinegar R, Schreiber W, Hugo R. Biphasic Development of carrageenin edema in rats. J Pharmacol Exp Ther. 1969;166:96–103.PubMed
46.
Larche J, Lancel S, Hassoun SM, Favory R, Decoster B, Marchetti P, Chopin C, Neviere R. Inhibition of Mitochondrial Permeability Transition Prevents Sepsis-Induced Myocardial Dysfunction and Mortality. J Am Coll Cardiol. 2006;48:377–85.CrossRefPubMed
47.
Hopkins SJ. The pathophysiological role of cytokines. Leg Med. 2003;5:45–57.CrossRef
48.
Banki E, Hajna Z, Kemeny A, Botz B, Nagy P, Bolcskei K, Toth G. The selective PAC1 receptor agonist maxadilan inhibits neurogenic vasodilation and edema formation in the mouse skin. Neuropharmacology. 2014;85:538–47.CrossRefPubMed
49.
Barry CM, Helps SC, Den HCV, Vink R. Characterizing the role of the neuropeptide substance P in experimental subarachnoid hemorrhage. Brain Res. 2011;1389:143–51.CrossRefPubMed
50.
Pathak D, Pathak K, Singla AK. Flavonoids as medicinal agents: recent advances. Fitoterapia. 1991;557:371–89.
51.
Cabrera PV, Blanco G, Gravisaco MJ, Alvarez E, Hajos S. Zymosan Modulates CD44 Isoform Expression in a Murine Model of Inflammation Resembling Rheumatoid Arthritis Synovitis. J Rheumatol. 2001;28:944–9.
52.
Makni-Maalej K, Chiandotto M, Hurtado-Nedelec M, Bedouhene S, Gougerot-Pocidalo MA, Dang PMC, El-Benna J. Zymosan induces NADPH oxidase activation in human neutrophils by inducing the phosphorylation of p47phox and the activation of Rac2: Involvement of protein tyrosine kinases, PI3Kinase, PKC, ERK1/2 and p38MAPkinase. Biochem Pharmacol. 2013;85:92–100.CrossRefPubMed
53.
Taylor PR, Brown GD, Reid DM, Willment JA, Martinez-Pomares L, Gordon S, Wong SYC. The Glucan Receptor, Dectin-1, Is Predominantly Expressed on the Surface of Cells of the Monocyte/Macrophage and Neutrophil Lineages. J Immunol. 2002;169:3876–82.CrossRefPubMed
54.
Guerrero ATG, Cunha TM, Verri W, Gazzinelli RT, Teixeira MM, Cunha FQ, Ferreira SH. Toll-like receptor 2/MyD88 signaling mediates zymosan-induced joint hypernociception in mice: Participation of TNF-α, IL-1β and CXCL1/KC. Eur J Pharmacol. 2011;674:51–7.CrossRefPubMed
55.
Choi S, Lim T, Kyung M, Kim Y, Kim J, Joo N, Su T, Park J, Hun M, Won K. Rutin inhibits B [a] PDE-induced cyclooxygenase-2 expression by targeting EGFR kinase activity. Biochem Pharmacol. 2013;86:1468–75.CrossRefPubMed
56.
Shan J, Fu J, Zhao Z, Kong X, Huang H, Luo L, Yin Z. Chlorogenic acid inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in RAW264.7 cells through suppressing NF-kappaB and JNK/AP-1 activation. Int Immunopharmacol. 2009;9:1042–8.CrossRefPubMed
57.
Pick R, Brechtefeld D, Walzog B. Intraluminal crawling versus interstitial neutrophil migration during inflammation. Mol Immunol. 2013;55:70–5.CrossRefPubMed
Metadata
Title
Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae)
Authors
Manoela Torres-Rêgo
Allanny Alves Furtado
Mariana Angélica Oliveira Bitencourt
Maira Conceição Jerônimo de Souza Lima
Rafael Caetano Lisbôa Castro de Andrade
Eduardo Pereira de Azevedo
Thaciane da Cunha Soares
José Carlos Tomaz
Norberto Peporine Lopes
Arnóbio Antônio da Silva-Júnior
Silvana Maria Zucolotto
Matheus de Freitas Fernandes-Pedrosa
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1259-x

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