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BMC Complementary Medicine and Therapies

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Open Access 01-12-2016 | Research article

Protective effect of fermented Cyclopia intermedia against UVB-induced damage in HaCaT human keratinocytes

Authors: A-Rang Im, Sung Hum Yeon, Jung Seung Lee, Key An Um, Young -Joon Ahn, Sungwook Chae

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Abstract

Background

The fermented leaves and stems of Cyclopia intermedia are used to brew honeybush tea, a herbal tea indigenous to South Africa. The aim of this study was to evaluate the protective effect of fermented honeybush extracts (FH ex) and scale-up fermented honeybush extracts (SFH ex) against ultraviolet B (UVB)-induced damage in HaCaT keratinocytes. To this end, we examined UVB-induced cell viability, antioxidant enzymes, and inflammatory mediators in HaCaT cells.

Methods

UVB significantly decreased HaCaT cell viability, whereas FH ex and SFH ex did not exhibit cytotoxic effects and increased the viability of the HaCaT cells. To further investigate the protective effects of FH ex on UVB-induced oxidative stress in HaCaT cells, the activities of superoxide dismutase (SOD), catalase (CAT), matrix metalloproteinases (MMPs), pro-inflammatory cytokines and skin barrier function in terms of involucrin, filaggrin, and loricrin were analyzed.

Results

UVB-induced treatment reduced the activity of antioxidant enzymes and skin barrier function, while FH ex or SFH ex increased their activity. These results suggest that FH ex exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through stimulation of antioxidant enzymes activities. Furthermore, FH ex and SFH ex suppressed the UVB-induced expression of inflammatory mediators, such as IL-1β, IL-6, and IL-8, at mRNA level together with down regulation of matrix metalloproteinase (MMPs). In addition, FH ex and SFH ex reversed the phosphorylation of mitogen-activated protein kinase (MAPK) induced by UVB-irradiation. Notably, FH ex and SFH ex markedly inhibited UVB-induced activation of ERK, p38, and JNK. Thus, this agent exhibits anti-oxidative and -inflammatory effects via lowering ROS production, suppressing p38, ERK, and JNK activation, and down-regulating expression of MMPs.

Conclusions

These findings suggest that FH ex and SFH ex can be used as a skin anti-photoaging agent.

Kohl E, Steinbauer J, Landthaler M, Szeimies RM. Skin ageing. J Eur Acad Dermatol Venereol. 2011;25(8):873–84.CrossRefPubMed

Helfrich YR, Sachs DL, Voorhees JJ. Overview of skin aging and photoaging. Dermatol Nurs. 2008;20(3):177–83. quiz 184.PubMed

Bernstein EF, Chen YQ, Tamai K, Shepley KJ, Resnik KS, Zhang H, et al. Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J Invest Dermatol. 1994;103(2):182–6.CrossRefPubMed

Poljšak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatol Res Pract. 2012;2012:135206.PubMedPubMedCentral

Waris G, Ahsan H. Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog. 2006;5:14.CrossRefPubMedPubMedCentral

Cho HS, Lee MH, Lee JW, No KO, Park SK, Lee HS, et al. Anti-wrinkling effects of the mixture of vitamin C, vitamin E, pycnogenol and evening primrose oil, and molecular mechanisms on hairless mouse skin caused by chronic ultraviolet B irradiation. Photodermatol Photoimmunol Photomed. 2007;23(5):155–62.CrossRefPubMed

Pandel R, Poljšak B, Godic A, Dahmane R. Skin photoaging and the role of antioxidants in its prevention. ISRN Dermatol. 2013;2013:930164.CrossRefPubMedPubMedCentral

Brenneisen P, Sies H, Scharffetter-Kochanek K. Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann N Y Acad Sci. 2002;973:31–43.CrossRefPubMed

Quan T, Qin Z, Xia W, Shao Y, Voorhees JJ, Fisher GJ. Matrix-degrading metalloproteinases in photoaging. J Investig Dermatol Symp Proc. 2009;14(1):20–4.CrossRefPubMedPubMedCentral

10.

Ishida T, Sakaguchi I. Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon. Biol Pharm Bull. 2007;30(5):928–34.CrossRefPubMed

11.

Bickers DR, Athar M. Oxidative stress in the pathogenesis of skin disease. J Invest Dermatol. 2006;126(12):2565–75.CrossRefPubMed

12.

Röck K, Meusch M, Fuchs N, Tigges J, Zipper P, Fritsche E, et al. Estradiol protects dermal hyaluronan/versican matrix during photoaging by release of epidermal growth factor from keratinocytes. J Biol Chem. 2012;287(24):20056–69.CrossRefPubMedPubMedCentral

13.

Steinert PM, Marekov LN. The proteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope. J Biol Chem. 1995;270(30):17702–11.CrossRefPubMed

14.

Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010;48(12):909–30.CrossRefPubMed

15.

Song JL, Gao Y. Protective effects of Lindera coreana on UVB-induced oxidative stress in human HaCaT keratinocytes. Iran J Pharm Res. 2014;13(4):1369–78.PubMedPubMedCentral

16.

de Beer D, Schulze AE, Joubert E, de Villiers A, Malherbe CJ, Stander MA. Food ingredient extracts of Cyclopia subternata (Honeybush): variation in phenolic composition and antioxidant capacity. Molecules. 2012;17(12):14602–24.CrossRefPubMed

17.

Kokotkiewicz A, Luczkiewicz M. Honeybush (Cyclopia sp.)-a rich source of compounds with high antimutagenic properties. Fitoterapia. 2009;80(1):3–11.CrossRefPubMed

18.

Marnewick JL, van der Westhuizen FH, Joubert E, Swanevelder S, Swart P, Gelderblom WC. Chemoprotective properties of rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia) herbal and green and black (Camellia sinensis) teas against cancer promotion induced by fumonisin B1 in rat liver. Food Chem Toxicol. 2009;47(1):220–9.CrossRefPubMed

19.

Im AR, Song JH, Lee MY, Yeon SH, Um KA, Chae S. Anti-wrinkle effects of fermented and non-fermented Cyclopia intermedia in hairless mice. BMC Complement Alterna Med. 2014;14:424.CrossRef

20.

Allemann IB, Baumann L. Botanicals in skin care products. Int J Dermatol. 2009;48(9):923–34.CrossRefPubMed

21.

Stallings AF, Lupo MP. Practical uses of botanicals in skin care. J Clin Aesthet Dermatol. 2009;2(1):36–40.PubMedPubMedCentral

22.

Camouse MM, Domingo DS, Swain FR, Conrad EP, Matsui MS, Maes D, et al. Topical application of green and white tea extracts provides protection from solar-simulated ultraviolet light in human skin. Exp Dermatol. 2009;18(6):522–6.CrossRefPubMed

23.

Leon AC, Davis LL, Kraemer HC. The role and interpretation of pilot studies in clinical research. J Psychiatr Res. 2011;45(5):626–9.CrossRefPubMed

24.

Dai J, Ma H, Fan J, Li Y, Wang J, Ni H, et al. Crude polysaccharide from an anti-UVB cell clone of Bupleurum scorzonerifolium protect HaCaT cells against UVB-induced oxidative stress. Cytotechnology. 2011;63(6):599–607.CrossRefPubMedPubMedCentral

25.

Shindo Y, Witt E, Packer L. Antioxidant defense mechanisms in murine epidermis and dermis and their responses to ultraviolet light. J Invest Dermatol. 1993;100(3):260–5.CrossRefPubMed

26.

Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ. Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med. 1997;337(20):1419–28.CrossRefPubMed

27.

Tzaphlidou M. The role of collagen and elastin in aged skin: an image processing approach. Micron. 2004;35(3):173–7.CrossRefPubMed

28.

Quan T, Little E, Quan H, Voorhees JJ, Fisher GJ. Elevated matrix metalloproteinases and collagen fragmentation in photodamaged human skin: Impact of altered extracellular matrix microenvironment on dermal fibroblast function. J Invest Dermatol. 2013;133(5):1362–6.CrossRefPubMedPubMedCentral

29.

Jenkins G. Molecular mechanisms of skin ageing. Mech Ageing Dev. 2002;123(7):801–10.CrossRefPubMed

30.

Cho S, Kim HH, Lee MJ, Lee S, Park CS, Nam SJ, et al. Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo. J Lipid Res. 2008;49(6):1235–45.CrossRefPubMed

31.

Hwang E, Sun ZW, Lee TH, Shin HS, Park SY, Lee DG, et al. Enzyme-processed Korean Red Ginseng extracts protects against skin damage induced by UVB irradiation in hairless mice. J Ginseng Res. 2013;37(4):425–34.CrossRefPubMedPubMedCentral

32.

Bosset S, Bonnet-Duquennoy M, Barré P, Chalon A, Lazou K, Kurfurst R, et al. Decreased expression of keratinocyte beta1 integrins in chronically sun-exposed skin in vivo. Br J Dermatol. 2003;148(4):770–8.CrossRefPubMed

33.

Rawlings AV, Matts PJ. Stratum corneum moisturization at the molecular level: an update in relation to the dry skin cycle. J Invest Dermatol. 2005;124(6):1099–110.CrossRefPubMed

34.

Bernerd F, Asselineau D. Successive alteration and recovery of epidermal differentiation and morphogenesis after specific UVB-damages in skin reconstructed in vitro. Dev Biol. 1997;183(2):123–38.CrossRefPubMed

35.

Lee C, Park GH, Ahn EM, Park CI, Jang JH. Sargassum fulvellum protects HaCaT cells and BALB/c mice from UVB-induced proinflammatory responses. Evid Based Complement Alternat Med. 2013;2013:747846.PubMedPubMedCentral

36.

Kirnbauer R, Köck A, Neuner P, Förster E, Krutmann J, Urbanski A, et al. Regulation of epidermal cell interleukin-6 production by UV light and corticosteroids. J Invest Dermatol. 1991;96(4):484–9.CrossRefPubMed

37.

Fisher GJ, Talwar HS, Lin J, Lin P, McPhillips F, Wang Z, et al. Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo. J Clin Invest. 1998;101(6):1432–40.CrossRefPubMedPubMedCentral

Title: Protective effect of fermented Cyclopia intermedia against UVB-induced damage in HaCaT human keratinocytes
Authors: A-Rang Im
Sung Hum Yeon
Jung Seung Lee
Key An Um
Young -Joon Ahn
Sungwook Chae
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-016-1218-6

At a glance: The STEP trials

Springer Medicine

Protective effect of fermented Cyclopia intermedia against UVB-induced damage in HaCaT human keratinocytes

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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