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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Antibacterial constituents of Fructus Chebulae Immaturus and their mechanisms of action

Authors: Kun Li, Yue Lin, Bin Li, Taowen Pan, Fei Wang, Ruqiang Yuan, Jianjun Ji, Yunpeng Diao, Shouyu Wang

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

To extract, purify, and identify the effective constituents of aqueous extract of Fructus Chebulae Immaturus, and analyze the bactericidal effects of total tannins.

Methods

Preparative thin layer chromatography and semi-preparative high performance liquid chromatography were used to isolate and purify the total tannin fraction. 1H- and 13C- NMR spectroscopy were used to elucidate compound structures. The antibacterial activities of total tannins and ethyl gallate on Klebsiella pneumoniae (KP) and Staphylococcus aureus (SA) were determined through minimum inhibitory concentration and minimum bactericidal concentration assays. Their antibacterial mechanisms of action were explored by transmission electron microscopy and scanning electron microscopy.

Results

Five compounds were isolated: ellagic acid, ethyl gallate, arjugenin, β-sitosterol, and tri-n-butyl chebulate. Tri-n-butyl chebulate is a newly-reported compound. Total tannins and ethyl gallate both had favorable bactericidal effects against KP and SA.

Conclusion

In vivo and in vitro pharmacodynamic experiment demonstrated that the effective components of Fructus Chebulae Immaturus possessed significant antibacterial effects, and were nontoxic and safe.

Trial registration

No results of a health care intervention on human participants
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Metadata
Title
Antibacterial constituents of Fructus Chebulae Immaturus and their mechanisms of action
Authors
Kun Li
Yue Lin
Bin Li
Taowen Pan
Fei Wang
Ruqiang Yuan
Jianjun Ji
Yunpeng Diao
Shouyu Wang
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1162-5

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