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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Decatropis bicolor (Zucc.) Radlk essential oil induces apoptosis of the MDA-MB-231 breast cancer cell line

Authors: C. C. Estanislao Gómez, A. Aquino Carreño, D. G. Pérez Ishiwara, E. San Martín Martínez, J. Morales López, N. Pérez Hernández, M. C. Gómez García

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

Decatropis bicolor (Zucc.)Radlk is a plant that has been traditionally used for the treatment of breast cancer in some communities of Mexico. So, the aim of this study was to determine the cytotoxic and apoptotic effect of the essential oil of Decatropis bicolor against breast cancer cell line, MDA-MB-231.

Methods

The essential oil obtained from hydrodestillation of leaves of Decatropis bicolor was studied for its biological activity against breast cancer cells MDA-MB-231 by MTT assay, Hematoxylin-eosin stain, Annexin V-FITC, TUNEL and western blot assays and for its chemical composition by GC-MS.

Results

The results showed a relevant cytotoxic effect of the essential oil towards MDA-MB-231 cells in a dose- and time- dependent manner, with an IC50 of 53.81 ± 1.691 μg/ml but not in the epithelial mammary cell line MCF10A (207.51 ± 3.26 μg/ml). Morphological examination displayed apoptotic characteristics in the treated cells like cell size reduction, membrane blebbing and apoptotic bodies. In addition, the apoptotic rate significantly increased as well as DNA fragmentation and western blot analysis revealed that the essential oil induced apoptosis in the MDA-MB-231 cells via intrinsic pathways due to the activation of Bax, caspases 9 and 3. Phytochemical analysis of the Decatropis bicolor essential oil showed the presence of twenty-three compounds. Major components of the oil were 1,5-cyclooctadiene,3-(methyl-2)propenyl (18.38 %), β-terpineol (8.16 %) and 1-(3-methyl-cyclopent-2-enyl)-cyclohexene (6.12 %).

Conclusions

This study suggests that essential oil of Decatropis bicolor has a potential cytotoxic and antitumoral effect against breast cancer cells, with the presence of potential bioactive compounds. Our results contribute to the validation of the anticancer activity of the plant in Mexican traditional medicine.
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Literature
1.
Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F. Cancer incidence and mortality Worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136:E359–86. doi:10.​1002/​ijc.​29210.CrossRefPubMed
2.
Pandey G, Madhuri S. Medicinal plants: better remedy for neoplasm. Indian Drugs. 2006;43:869–74.
3.
4.
5.
Prema R, Sathish Sekar D, Chandra Sekhar KB. Review on: herbs as anticancer agents. Int J Pharma & Ind Res. 2011;1(2):105–8.
6.
Bayala B, Bassole IH, Scifo R, Gnoula C, Morel L, Lobaccaro JM, et al. Anticancer activity of essential oils and their chemical components-a review. Am J Cancer Res. 2014;4(6):591–607.PubMedPubMedCentral
7.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils-a review. Food Chem Toxicol. 2008;46:446–75.CrossRefPubMed
8.
Edris AE. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytothe Res. 2007;21(4):308–23.CrossRef
9.
Smith N, Mori A, Henderson DW, Stevenson DW, Heald SV. Flowering Plants of the Neotropics. New Jersey: Princeton University Press; 2004. p. 333–5.
10.
Misharina TA, Samusenko AL. Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove, and their mixtures. Appl Biochem Microbiol. 2008;45(4):438–42.CrossRef
11.
Odeh F, Rahmo A, Alnory SA, Chaty EM. The cytotoxic effect of essential oils Citrus aurantium peels on human colorectal carcinoma cell line (Lim1863). J Microbiol Biotechnol Food Sci. 2012;1(6):1476–87.
12.
Berliocchi L, Ciociaro A, Russo R, Cassiano MG, Blandini F, Rotiroti D, et al. Toxic profile of bergamot essential oil on survival and proliferation of SH-SY5Y neuroblastoma cells. Food Chem Toxicol. 2011;49:2780–92.CrossRefPubMed
13.
Cárdenas ON, Pérez GS, Zavala SM, Aguirre RJ, Pérez GC. Actividad antifúngica de seis plantas sobre Aspergillus flavus. Revista Mexicana de Ciencias Farmacéuticas. 2005;36(03):21–6.
14.
García-Argáez AN, Ramírez Apan TO, Parra Delgado H, Velázquez G, Martínez-Vázquez M. Anti-inflammatory activity of coumarins from Decatropis bicolor on TPA ear mice model. Planta Med. 2000;66(3):279–81.CrossRefPubMed
15.
Sánchez GA, Granados SD, Simón NR. Uso medicinal de las plantas por los otomíes del municipio de Nicolás Flores, Hidalgo. México Revista Chapingo Serie Horticultura. 2008;14(3):271–9.
16.
17.
Pérez EB, Villavicencio NM. Listado de plantas medicinales del Estado de Hidalgo. Pachuca: Universidad Autónoma del Estado de Hidalgo; 1995. p. 45.
18.
19.
Calvo-Gómez O, Morales-López J, López M. Solid-phase microextraction–gas chromatographic–mass spectrometric analysis of garlic oil obtained by hydrodistillation. J Chromatogr A. 2004;1036(1):91–3.CrossRefPubMed
20.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Meth. 1983;65:55–63.CrossRef
21.
Wang W, Li N, Luo M, Zu Y, Efferth T. Antibacterial activity and anticancer activity of Rosmarinus officinalis L. essential oil compared to that of its main components. Molecules. 2012;17(3):2704–13.CrossRefPubMed
22.
Atti-Santos AC, Rossato M, Serafini LA, Cassel E, Moyna P. Extraction of essential oils from lime (citrus latifolia Tanaka) by hydrodistillation and supercritical carbon dioxide. Braz Arch Biol Technol. 2005;48(1):155–60.CrossRef
23.
Macías VE, Coy BE, Cuca SL. Preliminary phytochemical analysis and antioxidant, antiinflammatory and antiproliferative activities of the bark ethanol extracts of Zanthoxylum fagara (L.) Sarg. (Rutaceae). Rev Cubana Plant Med. 2011;16(1):43–53.
24.
Fadlalla K, Watson A, Yehualaeshet T, Turner T, Smuel T. Ruta graveolens extract induces DNA damage pathways and blocks Akt activation to inhibit cancer cell proliferation and survival. Anticancer Res. 2011;31:233–41.PubMedPubMedCentral
25.
Starmans DAJ, Nijhuis HH. Extraction of secondary metabolites from plant material: a review. Trends Food Sci Techol. 1996;7:191–7.CrossRef
26.
Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007;70(3):461–77.CrossRefPubMed
27.
Charafe-Jauffret E, Ginestier C, Monville F, Finetti P, Adélaïde J, Cervera N, et al. Gene expression profiling of breast cell lines identifies potential new basal markers. Oncogene. 2006;25(15):2273–84.CrossRefPubMed
28.
Vermeulen K, Van Bockstaele DR, Berneman ZN. Apoptosis: mechanisms and relevance in cancer. Ann Hematol. 2005;84:627–39.CrossRefPubMed
29.
30.
31.
Marzo I, Naval J. Bcl-2 family members as molecular targets in cancer therapy. Biochem Pharmacol. 2008;76:939–46.CrossRefPubMed
32.
33.
Bicas JL, Neri-Numa IA, Ruiz AL, De Carvalho JE, Pastore GM. Evaluation of the antioxidant and antiproliferative potential of bioflavors. Food Chem Toxicol. 2011;49:1610–5.CrossRefPubMed
34.
35.
Loizzo MR, Tundis R, Menichini F, Saab AM, Statti GA, Menichini F. Antiproliferative effects of essential oils and their major constituents in human renal adenocarcinoma and amelanotic melanoma cells. Cell Prolif. 2008;41:1002–12.CrossRefPubMed
36.
37.
Usta J, Kreydiyyeh S, Knio K, Barnabe P, Bou-Moughlabay Y, Dagher S. Linalool decreases HepG2 viability by inhibiting mitochondrial complexes I and II, increasing reactive oxygen species and decreasing ATP and GSH levels. Chem Biol Interact. 2009;180(1):39–46.CrossRefPubMed
38.
39.
Palazzo MC, Agius BR, Wright BS, Haber WA, Moriarity DM, Setzer WN. Chemical compositions and cytotoxic activities of leaf essential oils of four lauraceae tree species from Monteverde, Costa Rica. Rec Nat Prod. 2009;3(1):32–7.
Metadata
Title
Decatropis bicolor (Zucc.) Radlk essential oil induces apoptosis of the MDA-MB-231 breast cancer cell line
Authors
C. C. Estanislao Gómez
A. Aquino Carreño
D. G. Pérez Ishiwara
E. San Martín Martínez
J. Morales López
N. Pérez Hernández
M. C. Gómez García
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1136-7

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