Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2015 | Research article

Antibacterial and antibiotic-modifying activities of three food plants (Xanthosoma mafaffa Lam., Moringa oleifera (L.) Schott and Passiflora edulis Sims) against multidrug-resistant (MDR) Gram-negative bacteria

Authors: Joachim K. Dzotam, Francesco K. Touani, Victor Kuete

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Login to get access

Abstract

Background

The present study was designed to investigate the antibacterial activities of the methanol extract of three edible plants, namely Xanthosoma mafaffa, Moringa oleifera and Passiflora edulis and their synergistic effects with some commonly used antibiotics against MDR Gram-negative bacteria expressing active efflux pumps.

Methods

Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the extracts, as well as those of antibiotics in association with the extracts.

Results

The phytochemical test indicate that all tested crude extracts contained polyphenols, triterpenes and steroids whilst other phytochemical classes were selectively distributed. Extracts showed antibacterial activities with minimum inhibitory concentrations ranging from 128-1024 μg/mL on the majority of the 19 tested Gram-negative bacterial strains. Extract from the pericarp of P. edulis inhibited the growth of 89.5 % of the 19 tested bacterial strains, the lowest minimal inhibitory concentration (MIC) value of 128 μg/mL being recorded against Escherichia coli AG100 strain. In the presence of Phenylalanine-Arginine β-Naphtylamide (PAβN)], an efflux pump inhibitor (EPI), the activity of the extract from X. mafaffa increased on 40 % of tested strains. In combination with antibiotics, extracts of X. mafaffa, M. oleifera and pericarp of P. edulis showed synergistic effects with some antibiotics against more than 75 % of the tested bacteria.

Conclusion

The results of the present study indicate that the tested plants may be used in the treatment of bacterial infections including the multi-resistant bacteria.
Literature
1.
2.
Poole K. Mechanisms of bacterial biocide and antibiotic resistance. J Appl Microbiol. 2002;92(Suppl):55S–64.CrossRefPubMed
3.
4.
Fankam AG, Kuete V, Voukeng IK, Kuiate JR, Pages JM. Antibacterial activities of selected Cameroonian spices and their synergistic effects with antibiotics against multidrug-resistant phenotypes. BMC Complement Altern Med. 2011;11:104.CrossRefPubMedPubMedCentral
5.
Voukeng IK, Kuete V, Dzoyem JP, Fankam AG, Noumedem JA, Kuiate JR, et al. Antibacterial and antibiotic-potentiation activities of the methanol extract of some cameroonian spices against Gram-negative multi-drug resistant phenotypes. BMC Res Notes. 2012;5:299.CrossRefPubMedPubMedCentral
6.
Djeussi DE, Noumedem JA, Seukep JA, Fankam AG, Voukeng IK, Tankeo SB, et al. Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria. BMC Complement Altern Med. 2013;13:164.CrossRefPubMedPubMedCentral
7.
Seukep JA, Fankam AG, Djeussi DE, Voukeng IK, Tankeo SB, Noumdem JA, et al. Antibacterial activities of the methanol extracts of seven Cameroonian dietary plants against bacteria expressing MDR phenotypes. Springerplus. 2013;2:363.CrossRefPubMedPubMedCentral
8.
Kuete V, Ngameni B, Tangmouo JG, Bolla JM, Alibert-Franco S, Ngadjui BT, et al. Efflux pumps are involved in the defense of Gram-negative bacteria against the natural products isobavachalcone and diospyrone. Antimicrob Agents Chemother. 2010;54:1749–52.CrossRefPubMedPubMedCentral
9.
Kuete V, Alibert-Franco S, Eyong KO, Ngameni B, Folefoc GN, Nguemeving JR, et al. Antibacterial activity of some natural products against bacteria expressing a multidrug-resistant phenotype. Int J Antimicrob Agents. 2011;37:156–61.CrossRefPubMed
10.
Noumedem JA, Mihasan M, Lacmata ST, Stefan M, Kuiate JR, Kuete V. Antibacterial activities of the methanol extracts of ten Cameroonian vegetables against Gram-negative multidrug-resistant bacteria. BMC Complement Altern Med. 2013;13:26.CrossRefPubMedPubMedCentral
11.
Noumedem JA, Mihasan M, Kuiate JR, Stefan M, Cojocaru D, Dzoyem JP, et al. In vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species. BMC Complement Altern Med. 2013;13:190.CrossRefPubMedPubMedCentral
12.
Fankam AG, Kuiate JR, Kuete V. Antibacterial activities of Beilschmiedia obscura and six other Cameroonian medicinal plants against multi-drug resistant Gram-negative phenotypes. BMC Complement Altern Med. 2014;14:241.CrossRefPubMedPubMedCentral
13.
Touani FK, Seukep AJ, Djeussi DE, Fankam AG, Noumedem JA, Kuete V. Antibiotic-potentiation activities of four Cameroonian dietary plants against multidrug-resistant Gram-negative bacteria expressing efflux pumps. BMC Complement Altern Med. 2014;14:258.CrossRefPubMedPubMedCentral
14.
Harbone J. Phytochemical methods: a guide to modern techniques of plant analysis. London: Chapman & Hall; 1973.
15.
Ngameni B, Fotso GW, Kamga J, Ambassa P, Abdou T, Fankam AG, et al. 9 - Flavonoids and Related Compounds from the Medicinal Plants of Africa. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 301–50.CrossRef
16.
Wansi JD, Devkota KP, Tshikalange E, Kuete V. 14 - Alkaloids from the Medicinal Plants of Africa. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 557–605.CrossRef
17.
Poumale HMP, Hamm R, Zang Y, Shiono Y, Kuete V. 8 - Coumarins and Related Compounds from the Medicinal Plants of Africa. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 261–300.CrossRef
18.
Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998;64:711–3.CrossRefPubMed
19.
Mativandlela SPN, Lall N, Meyer JJM. Antibacterial, antifungal and antitubercular activity of (the roots of) Pelargonium reniforme (CURT) and Pelargonium sidoides (DC) (Geraniaceae) root extracts. S Afr J Bot. 2006;72:232–7.CrossRef
20.
Lacmata ST, Kuete V, Dzoyem JP, Tankeo SB, Teke GN, Kuiate JR, et al. Antibacterial activities of selected Cameroonian plants and their synergistic effects with antibiotics against bacteria expressing MDR phenotypes. Evid Based Complement Altern Med. 2012;2012:623723.CrossRef
21.
Kuete V, Kamga J, Sandjo LP, Ngameni B, Poumale HM, Ambassa P, et al. Antimicrobial activities of the methanol extract, fractions and compounds from Ficus polita Vahl. (Moraceae). BMC Complement Altern Med. 2011;11:6.CrossRefPubMedPubMedCentral
22.
Kuete V, Nana F, Ngameni B, Mbaveng AT, Keumedjio F, Ngadjui BT. Antimicrobial activity of the crude extract, fractions and compounds from stem bark of Ficus ovata (Moraceae). J Ethnopharmacol. 2009;124:556–61.CrossRefPubMed
23.
Kuete V, Wansi JD, Mbaveng AT, Kana Sop MM, Tadjong AT, Beng VP, et al. Antimicrobial activity of the methanolic extract and compounds from Teclea afzelii (Rutaceae). S Afr J Bot. 2008;74:572–6.CrossRef
24.
Kuete V, Ngameni B, Simo CC, Tankeu RK, Ngadjui BT, Meyer JJ, et al. Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae). J Ethnopharmacol. 2008;120:17–24.CrossRefPubMed
25.
Kuete V, Wabo GF, Ngameni B, Mbaveng AT, Metuno R, Etoa FX, et al. Antimicrobial activity of the methanolic extract, fractions and compounds from the stem bark of Irvingia gabonensis (Ixonanthaceae). J Ethnopharmacol. 2007;114:54–60.CrossRefPubMed
26.
Shahverdi AR, Monsef-Esfahani HR, Tavasoli F, Zaheri A, Mirjani R. Trans-Cinnamaldehyde from Cinnamomum zeylanicum Bark Essential Oil Reduces the Clindamycin Resistance of Clostridium difficile in vitro. J Food Sci. 2007;72:S055–8.CrossRefPubMed
27.
Braga LC, Leite AAM, Xavier KGS, Takahashi JA, Bemquerer MP, Chartone-Souza E, et al. Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Can J Microbiol. 2005;51:541–7.CrossRefPubMed
28.
Awouafack MD, Tane P, Kuete V, Eloff JN. 2 - Sesquiterpenes from the Medicinal Plants of Africa. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 33–103.CrossRef
29.
Ndhlala AR, Amoo SO, Ncube B, Moyo M, Nair JJ, Van Staden J. 16 - Antibacterial, antifungal, and antiviral activities of African medicinal plants. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 621–59.CrossRef
30.
Tsopmo A, Awah FM, Kuete V. 12 - Lignans and Stilbenes from African Medicinal Plants. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 435–78.CrossRef
31.
Vinoth B, Manivasagaperumal R, Balamurugan S. Phytochemical analysis and antibacterial activity of Moringa oleifera Lam. Int J Res Biol Sci. 2012;2:98–102.
32.
Mallea M, Chevalier J, Bornet C, Eyraud A, Davin-Regli A, Bollet C, et al. Porin alteration and active efflux: two in vivo drug resistance strategies used by Enterobacter aerogenes. Microbiology. 1998;144(Pt 11):3003–9.CrossRefPubMed
33.
Chevalier J, Pages JM, Eyraud A, Mallea M. Membrane permeability modifications are involved in antibiotic resistance in Klebsiella pneumoniae. Biochem Biophys Res Commun. 2000;274(2):496–9.CrossRefPubMed
34.
Pradel E, Pages JM. The AcrAB-TolC efflux pump contributes to multidrug resistance in the nosocomial pathogen Enterobacter aerogenes. Antimicrob Agents Chemother. 2002;46(8):2640–3.CrossRefPubMedPubMedCentral
35.
Mallea M, Mahamoud A, Chevalier J, Alibert-Franco S, Brouant P, Barbe J, et al. Alkylaminoquinolines inhibit the bacterial antibiotic efflux pump in multidrug-resistant clinical isolates. Biochem J. 2003;376(Pt 3):801–5.CrossRefPubMedPubMedCentral
36.
Tran QT, Mahendran KR, Hajjar E, Ceccarelli M, Davin-Regli A, Winterhalter M, et al. Implication of porins in beta-lactam resistance of Providencia stuartii. J Biol Chem. 2010;285(42):32273–81.CrossRefPubMedPubMedCentral
37.
38.
Ichimura T, Yamanaka A, Ichiba T, Toyokawa T, Kamada Y, Tamamura T, et al. Antihypertensive effect of an extract of Passiflora edulis rind in spontaneously hypertensive rats. Biosci Biotechnol Biochem. 2006;70:718–21.CrossRefPubMed
39.
Silva JR, Campos AC, Ferreira LM, Aranha Junior AA, Thiede A, Zago Filho LA, et al. [Extract of Passiflora edulis in the healing process of gastric sutures in rats: a morphological and tensiometric study]. Acta Cir Bras. 2006;21 Suppl 2:52–60.CrossRefPubMed
40.
Kannan S, Parimala B, Jayakar B. Antibacterial evaluation of the methanolic extract of Passiflora edulis. Hygeia JDMed. 2011;3(1):46–9.
41.
Fuglie L. The miracle tree Moringa oleifera: natural nutrition for the tropics. Dakar: Church World Service; 1999.
42.
Abrams B, Duncan D, Hertz-Picciotto I. A prospective study of dietary intake and acquired immune deficiency syndrome in HIV-seropositive homosexual men. J Acquir Immune Defic Syndr. 1993;6:949–58.PubMed
43.
Fahey J. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic and prophylactic properties. Trees for Life Journal. 2005;1:1–15.
44.
Viera GH, Mourao JA, Angelo AM, Costa RA, Vieira RH. Antibacterial effect (in vitro) of Moringa oleifera and Annona muricata against Gram positive and Gram negative bacteria. Rev Inst Med Trop Sao Paulo. 2010;52:129–32.CrossRefPubMed
Metadata
Title
Antibacterial and antibiotic-modifying activities of three food plants (Xanthosoma mafaffa Lam., Moringa oleifera (L.) Schott and Passiflora edulis Sims) against multidrug-resistant (MDR) Gram-negative bacteria
Authors
Joachim K. Dzotam
Francesco K. Touani
Victor Kuete
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-0990-7

Other articles of this Issue 1/2016

BMC Complementary Medicine and Therapies 1/2016 Go to the issue

Research article

Rice callus suspension culture inhibits growth of cell lines of multiple cancer types and induces apoptosis in lung cancer cell line

Research article

Translation and cross-cultural adaptation of a standardized international questionnaire on use of alternative and complementary medicine (I-CAM - Q) for Argentina

Research article

Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release

Research article

Trichloromethane fraction of Incarvillea compacta induces lytic cytotoxicity and apoptosis in Epstein-Barr virus-positive gastric cancer AGS cells

Research article

Use of complementary and alternative medicines in haemodialysis patients: a cross-sectional study from Palestine

Research article

Ethnopharmacological survey of herbal remedies used for treatment of various types of cancer and their methods of preparations in the West Bank-Palestine