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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2015

Open Access 01-12-2015 | Research article

Brazilian green propolis water extract up-regulates the early expression level of HO-1 and accelerates Nrf2 after UVA irradiation

Authors: Yuichi Saito, Kazuhiro Tsuruma, Kenji Ichihara, Masamitsu Shimazawa, Hideaki Hara

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

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Abstract

Background

Exposure to ultraviolet A (UVA) irradiation is the major cause of human skin aging. Suppression of UVA irradiation-induced skin fibroblast cell damage protects the skin against aging. An oxidative stress response transcription factor nuclear factor-(erythroid-derived 2)-related factor 2 (Nrf2) has an important role as a cytoprotective system against oxidative stress in the human skin and other organs. Propolis has been commonly used as a traditional medicine since ancient times. The water extract of propolis (WEP) mainly contains caffeoylquinic acids. In our previous study, we reported that WEP and its major constituents protected immortalized human skin fibroblast cells (NB1-RGB) against UVA irradiation-induced cell death. In this study, we examined the mechanism of WEP-mediated skin protection and the possible involvement of Nrf2/antioxidant response element (ARE) pathways.

Methods

Brazilian green propolis was used in the present study (Minas Gerais State, Brazil), Baccharis dracunculifolia is its main source. The Baccharis propolis was extracted with water at 50 °C to yield water extract. The NB1-RGB cell cultures were incubated for 23 h. After replenishing the medium, WEP or its constituents were added to the cell cultures. After 1 h, the cells were exposed to 10 J/cm2 of UVA light (365 nm UVA light source, CL-1000 L UV Closslinkers, Ultraviolet Products Ltd., Cambridge, UK). Heme oxygenase-1 (HO-1) expression levels in NB1-RGB cells were evaluated using western blotting. Nrf2 nuclear translocation changes in NB1-RGB cells were indicated using immunostaining.

Results

We demonstrated that WEP pretreatment up-regulated HO-1 expression level after UVA irradiation at earlier time points than vehicle pretreatment did, and three main constituents of WEP showed similar effects. Furthermore, WEP pretreatment also accelerated Nrf2 nuclear translocation after UVA irradiation.

Conclusions

Our findings indicated that WEP acts as an early inducer of HO-1 and rapid activator of Nrf2 to protect against UVA-induced oxidative stress.
Literature
1.
Khavkin J, Ellis DA. Aging Skin: Histology, Physiology, and Pathology. Facial Plast Surg Clin North Am. 2011;19:229–34.CrossRefPubMed
2.
Afanas’ev IB. Signaling by reactive oxygen and nitrogen species in skin diseases. Curr Drug Metab. 2010;11:409–14.CrossRefPubMed
3.
Bossi O, Gartsbein M, Leitges M, Kuroki T, Grossman S, Tennenbaum T. UV irradiation increases ROS production via PKCdelta signaling in primary murine fibroblasts. J Cell Biochem. 2008;105:194–207.CrossRefPubMed
4.
Amaro-Ortiz A, Yan B, D’Orazio JA. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules. 2014;19:6202–19.PubMedCentralCrossRefPubMed
5.
Beyer TA, Auf dem Keller U, Braun S, Schäfer M, Werner S. Roles and mechanisms of action of the Nrf2 transcription factor in skin morphogenesis, wound repair and skin cancer. Cell Death Differ. 2007;14:1250–4.CrossRefPubMed
6.
Nguyen T, Sherratt PJ, Pickett CB. Regulatory mechanisms controlling gene expression mediated by the antioxidant response element. Annu Rev Pharmacol Toxicol. 2003;43:233–60.CrossRefPubMed
7.
Wakabayashi N, Dinkova-Kostova AT, Holtzclaw WD, Kang M-I, Kobayashi A, Yamamoto M, et al. Protection against electrophile and oxidant stress by induction of the phase 2 response: fate of cysteines of the Keap1 sensor modified by inducers. Proc Natl Acad Sci U S A. 2004;101:2040–5.PubMedCentralCrossRefPubMed
8.
Jaramillo M, Zhang D. The emerging role of the Nrf2–Keap1 signaling pathway in cancer. Genes Dev. 2013;2179–2191.
9.
Reisman SA, Chertow GM, Hebbar S, Vaziri ND, Ward KW, Meyer CJ. Bardoxolone Methyl Decreases Megalin and Activates Nrf2 in the Kidney. J Am Soc Nephrol. 2012;1663–1673.
10.
Pergola PE, Krauth M, Huff JW, Ferguson DA, Ruiz S, Meyer CJ, et al. Effect of bardoxolone methyl on kidney function in patients with T2D and stage 3b-4 CKD. Am J Nephrol. 2011;33:469–76.CrossRefPubMed
11.
Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol. 1999;64:235–40.CrossRefPubMed
12.
Bankova V, Marcucci MC, Simova S, Nikolova N, Kujumgiev A, Popov S. Antibacterial diterpenic acids from Brazilian propolis. Zeitschrift fur Naturforsch Sect C - J Biosci. 1996;51.
13.
Drago L, Mombelli B, De Vecchi E, Fassina MC, Tocalli L, Gismondo MR. In vitro antimicrobial activity of propolis dry extract. J Chemother. 2000;12:390–5.CrossRefPubMed
14.
Paulino N, Teixeira C, Martins R, Scremin A, Dirsch VM, Vollmar AM, et al. Evaluation of the analgesic and anti-inflammatory effects of a Brazilian green propolis. Planta Med. 2006;72:899–906.CrossRefPubMed
15.
Teixeira ÉW, Message D, Negri G, Salatino A, Stringheta PC. Seasonal variation, chemical composition and antioxidant activity of brazilian propolis samples. Evid Based Complement Altern Med. 2010;7(January 2008):307–15.CrossRef
16.
Banskota AH, Tezuka Y, Kadota S. Recent progress in pharmacological research of propolis. Phytother Res. 2001;561–571.
17.
Ishihara M, Naoi K, Hashita M, Itoh Y, Suzui M. Growth inhibitory activity of ethanol extracts of Chinese and Brazilian propolis in four human colon carcinoma cell lines. Oncol Rep. 2009;22:349–54.PubMed
18.
Mishima S, Narita Y, Chikamatsu S, Inoh Y, Ohta S, Yoshida C, et al. Effects of propolis on cell growth and gene expression in HL-60 cells. J Ethnopharmacol. 2005;99:5–11.CrossRefPubMed
19.
Murase H, Shimazawa M, Kakino M, Ichihara K, Tsuruma K, Hara H. The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells. Evid Based Complement Alternat Med. 2013;2013:238279.PubMedCentralCrossRefPubMed
20.
Zhong JL, Edwards GP, Raval C, Li H, Tyrrell RM. The role of Nrf2 in ultraviolet A mediated heme oxygenase 1 induction in human skin fibroblasts. Photochem Photobiol Sci. 2010;9:18–24.CrossRefPubMed
21.
Hirota A, Kawachi Y, Itoh K, Nakamura Y, Xu X, Banno T, et al. Ultraviolet A irradiation induces NF-E2-related factor 2 activation in dermal fibroblasts: Protective role in UVA-induced apoptosis. J Invest Dermatol. 2005;124:825–32.CrossRefPubMed
22.
Bensasson RV, Zoete V, Dinkova-Kostova AT, Talalay P. Two-step mechanism of induction of the gene expression of a prototypic cancer-protective enzyme by diphenols. Chem Res Toxicol. 2008;21:805–12.CrossRefPubMed
23.
Kumar H, Kim I-S, More SV, Kim B-W, Choi D-K. Natural product-derived pharmacological modulators of Nrf2/ARE pathway for chronic diseases. Nat Prod Rep. 2014;31:109–39.CrossRefPubMed
24.
Na HK, Kim EH, Jung JH, Lee HH, Hyun JW, Surh YJ. (-)-Epigallocatechin gallate induces Nrf2-mediated antioxidant enzyme expression via activation of PI3K and ERK in human mammary epithelial cells. Arch Biochem Biophys. 2008;476:171–7.CrossRefPubMed
25.
Andreadi CK, Howells LM, Atherfold PA, Manson MM. Involvement of Nrf2, p38, B-Raf, and nuclear factor-kappaB, but not phosphatidylinositol 3-kinase, in induction of hemeoxygenase-1 by dietary polyphenols. Mol Pharmacol. 2006;69:1033–40.PubMed
26.
Kim KC, Kang KA, Zhang R, Piao MJ, Kim GY, Kang MY, et al. Up-regulation of Nrf2-mediated heme oxygenase-1 expression by eckol, a phlorotannin compound, through activation of Erk and PI3K/Akt. Int J Biochem Cell Biol. 2010;42:297–305.CrossRefPubMed
27.
Jun Y-J, Lee M, Shin T, Yoon N, Kim J-H, Kim H-R. Eckol Enhances Heme Oxygenase-1 Expression through Activation of Nrf2/JNK Pathway in HepG2 Cells. Molecules. 2014;19:15638–52.CrossRefPubMed
28.
Rubiolo JA, Mithieux G, Vega FV. Resveratrol protects primary rat hepatocytes against oxidative stress damage: Activation of the Nrf2 transcription factor and augmented activities of antioxidant enzymes. Eur J Pharmacol. 2008;591:66–72.CrossRefPubMed
29.
Cheng AS, Cheng YH, Chiou CH, Chang TL. Resveratrol upregulates Nrf2 expression to attenuate methylglyoxal-induced insulin resistance in hep G2 cells. J Agric Food Chem. 2012;60:9180–7.CrossRefPubMed
30.
Hwang YP, Yun HJ, Chun HK, Chung YC, Kim HK, Jeong MH, et al. Protective mechanisms of 3-caffeoyl, 4-dihydrocaffeoyl quinic acid from Salicornia herbacea against tert-butyl hydroperoxide-induced oxidative damage. Chem Biol Interact. 2009;181:366–76.CrossRefPubMed
31.
Cao X, Xiao H, Zhang Y, Zou L, Chu Y, Chu X. 1,5-Dicaffeoylquinic acid-mediated glutathione synthesis through activation of Nrf2 protects against OGD/reperfusion-induced oxidative stress in astrocytes. Brain Res. 2010;1347:142–8.CrossRefPubMed
Metadata
Title
Brazilian green propolis water extract up-regulates the early expression level of HO-1 and accelerates Nrf2 after UVA irradiation
Authors
Yuichi Saito
Kazuhiro Tsuruma
Kenji Ichihara
Masamitsu Shimazawa
Hideaki Hara
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-015-0945-4

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