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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2015

Open Access 01-12-2015 | Research article

The anti-aging properties of a human placental hydrolysate combined with dieckol isolated from Ecklonia cava

Authors: Su Kil Jang, Do Ik Lee, Seung Tae Kim, Gwang Hoon Kim, Da Woon Park, Jung Youl Park, Daehee Han, Jae Kwon Choi, Yoon-bok Lee, Nam-Soo Han, Yun Bae Kim, Jeongsu Han, Seong Soo Joo

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

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Abstract

Backgrounds

In the present study, we aimed to examine the anti-aging properties of human placental hydrolysate (HPE) and dieckol (DE) from Ecklonia cava against free radical scavenging, muscle hypertrophy-related follistatin mRNA expression, amelioration of cognition-related genes and proteins, inhibition of collagenase-regulating genes, and elastinase activity.

Methods

The anti-aging effects were examined in human fibroblast (CCD986sk), mouse myoblast (C2C12), and neuroblastoma (N2a) cell models, by employing various assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) scavenging, hydroxyl radical-mediated oxidation, quantitative real-time polymerase chain reaction, enzyme activity, and immunocytochemistry observation.

Results

Our results show that HPE combined with DE (HPE:DE) strongly scavenged DPPH radicals and protected proteins against degradation by hydroxyl radical attack. HPE:DE effectively inhibited matrix metalloproteinase-1 expression, protein kinase C alpha expression, and elastinase activity. Furthermore, HPE:DE improved the expression of cognition-related genes (choline acetyltransferase and vesicular acetylcholine transporter). These events may proactively contribute to retard the aging processes and the abrupt physiological changes probably induced by mitochondrial dysfunction with aging.

Conclusions

Based on these findings, we conclude that the combined treatment of HPE:DE may be useful for anti-aging therapy in which the accumulation of oxidative damage is the main driving force.
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Metadata
Title
The anti-aging properties of a human placental hydrolysate combined with dieckol isolated from Ecklonia cava
Authors
Su Kil Jang
Do Ik Lee
Seung Tae Kim
Gwang Hoon Kim
Da Woon Park
Jung Youl Park
Daehee Han
Jae Kwon Choi
Yoon-bok Lee
Nam-Soo Han
Yun Bae Kim
Jeongsu Han
Seong Soo Joo
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-015-0876-0

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