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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2015

Open Access 01-12-2015 | Research article

Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

Authors: Jihed Boubaker, Imen Mokdad Bzeouich, Nouha Nasr, Hajer Ben Ghozlen, Nadia Mustapha, Kamel Ghedira, Leila Chekir-Ghedira

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

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Abstract

Background

Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.

Methods

The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.

Results

Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.

Conclusion

The tested extracts have a significant biological effect which may be due to their bioactive compounds.
Literature
1.
Ivanova D, Gerova D, Chervenkov T, Yankova T. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. Journal of Ethnopharmacology. 2005;96:145–50.CrossRefPubMed
2.
Yaar M, Wu C, Park HY, Panova L, Schutz G, Gilchrest BA. Bone morphogenetic protein-4, a novel modulator of melanogenesis. J Biol Chem. 2006;281:25307–14.CrossRefPubMed
3.
Miyazawa M, Tamura N. Inhibitory compound of tyrosinase activity from the sprout of Polygonum hydropiper L. (Benitade). Biological and Pharmaceutical Bulletin. 2007;30:595–7.CrossRefPubMed
4.
Zhong S, Wu Y, Soo-Mi A, Zhao J, Wang K, Yang S, et al. Depig- mentation of melanocytes by the treatment of extracts from traditional Chinese herbs: a cell culture assay. Biological and Pharmaceutical Bulletin. 2006;29:1947–51.CrossRefPubMed
5.
Goleniowski ME, Bongiovanni GA, Bongiovanni L, Palacio CO, Cantero JJ. Medicinal plants from the “Sierra de Comechingones”. Argentina Journal of Ethnopharmacology. 2006;107:324–41.CrossRefPubMed
6.
Maas JL, Galletta GJ. Ellagic acid, an anticarcinogen in fruits, especially in strawberries: a review. Hort Sci. 1991;26:10–4.
7.
Samina N, Russell GK, Ramaswamy N. New antiproliferative epoxysecosterols from Pseudopterogorgia Americana. Tetrahedron Lett. 2000;41:6035–40.CrossRef
8.
Hannum SM. Potential impact of strawberries on human health a review of the science. Crit Rev Sci Nutr. 2004;44:1–7.CrossRef
9.
Hoang MT, Eichenfield LF. The rising incidence of melanoma in children and adolescents. Dermatol Nurs. 2000;12(188–189):183–92.
10.
Rao YK, Madamanchi G, Shih-Hua F, Yew-Min. Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: a comparative study. Food Chem Toxicol. 2007;45:1770–6.CrossRefPubMed
11.
Boubaker J, Bhouri W, Ben Sghaier M, Bouhlel I, Skandrani I, Ghedira K, et al. Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis. Cancer Cell International. 2011. doi:10.​1186/​1475-2867-11-37.PubMedPubMedCentral
12.
Alesiani D, Cicconi R, Mattei M, Montesano C, Bei R, Canini A. Cell cycle arrest and differentiation induction by 5,7-dimethoxycoumarin in melanoma cell lines. Int J Oncol. 2008;32:425–34.PubMed
13.
Pottier-Alapetite G. Flowers of Tunisia: Angiosperms, dicotyledons, apetals, dialypetals Tunisia. Tunisia: Ministry of higher Educationb and Scientific Research and the Ministry of Agriculture; 1979. p. 456.
14.
Cheib M, Boukhris M. Flora succint and illustrated flora of the arid zone and Sahara of Tunisia. Tunisia official printing house. 1998; 49:43–44.
15.
Boubaker J, Skandrani I, Bouhlel I, Ben sghaier M, Neffati A, Ghedira K, et al. Mutagenic, antimutagenic and antioxidant potency of leaf extracts from Nitraria retusa. Food and Chem Toxicol. 2010;48:2283–90.CrossRef
16.
Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorometric assay; assessment of chemosensitivity testing. Cancer Res. 1987;47:936–42.PubMed
17.
Wang IK, Lin-Shiau SY, Lin JK. Induction of Apoptosis by Apigenin and Related Flavonoids Through Cytochrome c Release and Activation of Caspase-9 and Caspase-3 in Leukaemia HL-60 Cells. European Journal of Cancer. 1999;35(10):1517–25.CrossRefPubMed
18.
Twentyman PR, Fox NE, Rees JK. Chemosensitivity testing of fresh leukaemic cells using the MTT colourimetric assay. Br J Haematol. 1989;71:19–24.CrossRefPubMed
19.
Skandrani I, Pinon A, Simon A, Ghedira K, Chekir-Ghedira L. Chloroform extract from Moricandia arvensis inhibits growth of B16-F0 melanoma cells and promotes differentiation in vitro. Cell Proliferation. 2010;43:471–9.CrossRefPubMed
20.
Boubaker J, Bhouri W, Ben Sghaier M, Bouhlel I, Kriffi M, Skandrani I, et al. Flavonoids Products from Nitraria retusa Leaves Promote Lymphoblastoid Cells Apoptosis. Nutrition and Cancer. 2012;64(7):1095–102.CrossRef
21.
Martinez C, Yañez J, Rodríguez J, Canteras M, Alcaraz M, Vicente V. Inhibicion del crecimiento de la lınea de melanoma B16-F10 por diferentes compuestos polifeno'licos. Revista Española de Patología. 2001;34:317–24.
22.
Yanez J, Martınez C, Rodrıguez J, Canteras M, Alcaraz M, Vicente V. Efecto de seis flavonoides sobre el crecimiento de la lınea de melanoma humano SK. Mel-1. Oncologia. 2002;25:210–6.
23.
Skandrani I, Ben Sghaier M, Neffati A, Boubaker J, Bouhlel I, Kilani S, et al. Antigenotoxic and free radical scavenging activities ofextracts from Moricandia arvensis. Drug Chem Toxicol. 2007;30:361–82.CrossRefPubMed
24.
Yu JQ, Lei JQ, Yu HD, Cai X, Zou GL. Chemical composition and antimicrobial activity of essential oil of Scutellaria barbata. Phytochemistry. 2004;65:881–4.CrossRefPubMed
25.
Reed JC, Pellecchia M. Apoptosis-based therapies for hematologic malignancies. Blood. 2005;106:408–18.CrossRefPubMed
26.
Machado KE, Oliveira KN, Santos-Bubniak L, Licínio MA, Nunes RJ, Santos-Silva MC. Evaluation of apoptotic effect of cyclic imide derivatives on murine B16F10 melanoma cells. Bioorganic & Medicinal Chemistry:. 2011;19:6285–91.CrossRef
27.
Skandrani I, Bouhlel I, Limem I, Boubaker J, Bhouri W, Neffati A, et al. Ghedira-Chekir l: Moricandia arvensis extracts protect against DNA damage, mutagenesis in bacteria system and scavenge the superoxide anion. TIV. 2009;23:166–75.
28.
Edward M, Gold JA, MacKie RM. Different susceptibilities of melanoma cells to retinoic acid-induced changes in melanotic expression. Biochemical and Biophysical Research Communications. 1988;155:773–8.CrossRefPubMed
29.
Puri N, Eller MS, Byers HR, Dykstra S, Kubera J, Gilchrest BA. Telomerebased DNA damage responses: a new approach to melanoma. FASEB Journal. 2004;18:1373–81.CrossRefPubMed
30.
Jian D, Jiang D, Su J, et al. Diethylstilbestrol enhances melanogenesis via cAMP-PKA-mediating up-regulation of tyrosinase and MITF in mouse B16 melanoma cells. Steroids. 2011;76:1297–304.CrossRefPubMed
31.
Solano F, Briganti S, Picardo M, Ghanem GH. Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigm Cell Res. 2006;19:550–71.CrossRef
32.
Chaabane F, Pinon A, Simon A, Ghedira K, Chekir-Ghedira L. Phytochemical potential of Daphne gnidium in inhibiting growth of melanoma cells and enhancing melanogenesis of B16-F0 melanoma. Cell Biochem Funct. 2012. doi:10.​1002/​cbf.​2919.PubMed
33.
Nakashima S, Matsuda H, Oda Y, Nakamura S, Xu F, Yoshikawa M. Melanogenesis inhibitors from the desert plant Anastatica hierochuntica in B16 melanoma cells. Bioorganic and Medicinal Chemistry. 2010;18:2337–45.CrossRefPubMed
34.
Nguyen DH, Nguyen DTM, La LH, Yang SH, Lee HB, Kim HJ, et al. Depigmenting effect of Cinnamomum cassia Presl in B16-F10 melanoma cells. Korean Journal of Chemical Engineering. 2007;24:827–30.CrossRef
Metadata
Title
Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma
Authors
Jihed Boubaker
Imen Mokdad Bzeouich
Nouha Nasr
Hajer Ben Ghozlen
Nadia Mustapha
Kamel Ghedira
Leila Chekir-Ghedira
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-015-0743-z

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