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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2015

Open Access 01-12-2015 | Research article

The in vitro cytotoxic activity of ethno-pharmacological important plants of Darjeeling district of West Bengal against different human cancer cell lines

Authors: Bipransh K Tiwary, Sony Bihani, Anoop Kumar, Ranadhir Chakraborty, Runu Ghosh

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

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Abstract

Background

Plant derived components have attracted particular attention as an alternative source to battle several diseases including cancer. The variation in the climate, the geographical location and the rich ethnomedicinal traditions has made the Darjeeling Himalayas an abode of invaluable repository of traditional medicinal plants. In this study, we explored the in vitro anticancer properties of traditionally used medicinal plants from the Darjeeling hills against different human cancer cell lines.

Methods

The ethanolic leaf extracts of 30 medicinal plants were tested for their cytotoxicity against human breast adenocarcinoma cell line (MCF 7), human hepatocarcinoma cell line (HepG2) and human cervix adenocarcinoma cell line (HeLa). The cytotoxicity was evaluated by performing MTT assay, trypan blue exclusion assay and morphological assessment under phase contrast inverted microscope. For the extracts which tested positive, IC50 (the concentration that inhibited cell growth by 50%) was calculated. The extract(s) were further subjected to Thin Layer Chromatography (TLC) to determine their phytochemical profile.

Results

Out of the 30 plant extracts tested, five plants, Artemisia indica, Eupatorium odoratum, Eupatorium adenophorum, Maesa macrophylla and Phlogacanthus thyrsiformis showed a > 50% growth inhibition of cancer cell lines at a concentration of 50 μg/ml. The sensitivity to different extracts varied according to the cell type under investigation. Of these plants, Maesa macrophylla, exhibited the most potent cytotoxicity against HeLa and MCF7 cell with IC50 values of 9.55 μg/ml and 16.19 μg/ml respectively. Phytochemical analysis revealed the presence of coumarins, flavonoids, tannins, saponins, steroids and terpenes.

Conclusions

This is perhaps the first report of screening of traditional medicinal plants from Darjeeling district in West Bengal, India, for their cytotoxic activity against three human cancerous cell lines MCF7, HeLa and HepG2. The extracts of Maesa macrophylla significantly inhibited the growth of HeLa and MCF7 cancerous cell lines and constituted of multiple known biologically active compounds. The present study may provide the landmark for further exploration of M. macrophylla for its potent anticancer constituents.
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Literature
1.
Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics. CA Cancer J Clin. 2007;57:43–66.CrossRefPubMed
2.
Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin D. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 2010;127:2893–917.CrossRefPubMed
3.
Dikshit R, Gupta PC, Ramasundarahettige C, Gajalakshmi V, Aleksandrowicz L, Badwe R, et al. Cancer mortality in India: a nationally representative survey. Lancet. 2012;379:1807–16.CrossRefPubMed
4.
Ali I, Wani WA, Saleem K. Cancer scenario in India with future perspectives. Canc Ther. 2011;8:56–70.
5.
Gullett NP, Ruhul Amin AR, Bayraktar S, Pezzuto JM, Shin DM, Khuri FR, et al. Cancer prevention with natural compounds. Semin Oncol. 2010;37:258–81.CrossRefPubMed
6.
Shah U, Shah R, Acharya S, Acharya N. Novel anticancer agents from plant sources. Chin J Nat Med. 2013;11(1):16–23.CrossRef
7.
8.
Cirla A, Mann J. Combrestatins: from natural product to drug discovery. Nat Prod Rep. 2003;20:558–64.CrossRefPubMed
9.
Luduena RF. Multiple form of tubulin: different gene products and covalent modification. Int Rev Cytology. 1998;178(13):207–75.
10.
Itokawa H, Wang X, Lee K-H. Homoharringtonine and related compounds. In: Cragg GM, Kingston DGI, Newman D, editors. Anticancer agents from natural products. Boca Raton: Florida, Brunner-Routledge Psychology Press, Taylor & Francis Group; 2005. p. 47–70.
11.
Sultana S, Asif HM, Nazar HMI, Akhtar N, Rehman JU, Rehman RU. Medicinal plants combating against cancer - a green approach. Asian Pac J Cancer Prev. 2014;15(11):4385–94.CrossRefPubMed
12.
Yonzone R, Bhujel RB, Rai S. Genetic resources, current ecological status and altitude wise distribution of medicinal plants diversity of Darjeeling Himalaya of West Bengal, India. Asian Pac J Trop. 2012;S439-S445.
13.
Chhetri DR, Basnet D, Chiu PF, Kalikotay S, Chhetri G, Parajuli S. Current status of ethnomedicinal plants in the Darjeeling Himalaya. Curr Sci. 2005;89:265–8.
14.
Yonzone R, Rai S, Bhujel RB. Ethnomedicinal and aromatic plant diversity and resources of Darjeeling district of Eastern Himalaya in India. Int J Adv Pharmaceut Res. 2012;3(4):859–71.
15.
Rai A, Rai S, Yonzone R. Ethnomedicinal plants used by the people of Darjeeling hills in the Eastern Himalaya of India. Univ J Pharm. 2013;02(01):122–34.
16.
Sharma BC. In vitro antibacterial activity of certain folk medicinal plants from Darjeeling Himalayas used to treat microbial infections. J Pharmacognosy Phytother. 2013;2(4):1–4.
17.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1–2):55–63.CrossRefPubMed
18.
Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival: modification to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods. 1986;89:271–7.CrossRefPubMed
19.
Strober W. Trypan blue exclusion test of cell viability. In: Coligan JE, Kruisbeek AM, Margulis DH, Shevach EM, Strober W, editors. Current Protocols in Immunology. New York: John Wiley & Sons; 1992. p. A.3.3–4.
20.
Wagner H, Bladt S. Plant Drug Analysis. A Thin Layer Chromatography Atlas. 2nd ed. Müchen: Springer; 1984.
21.
Badola HK, Pradhan BK. Plants used in healthcare practices by Limboo tribe in South-West of Khangchendzonga Biosphere Reserve, Sikkim, India. Indian J Tradit Know. 2013;12:355–69.
22.
Idrisi MS, Badola HK, Singh R. Indigenous knowledge amd medicinal use of plnts by local communities in Rangit Valley, South Sikkim, India. Ne Bio. 2012;1(2):34–45.
23.
Chatterjee A. Study on the enraging severity of cancer in West Begal, India from 2003 to 2010. Asian J Epidemiol. 2011;4(1):23–7.CrossRef
24.
Fouche G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J. In vitro anticancer screening of South African plants. J Ethnopharmacol. 2008;119:455–61.CrossRefPubMed
25.
Ramzi AM, Ulrike L, Renate G, Bednarski JP. Studies of the in vitro anticancer, antimicrobial and antioxidant potentials of selected Yemeni medicinal plants from the island Soqotra. BMC Compl Alt Med. 2009;9:7.CrossRef
26.
Fadeyi SA, Fadeyi OO, Adejumo AA, Okoro C, Myles EL. In Vitro Anticancer Screening of 24 Locally Used Nigerian Medicinal Plants. BMC Compl Alt Med. 2013;13:79.CrossRef
27.
Mahavorasirikul W, Viyanant V, Chaijaroenkul W, Itharat A, Na-Bangchang K. Cytotoxic activity of Thai medicinal plants against human cholangiocarcinoma, laryngeal and hepatocarcinoma cells in vitro. BMC Compl Alt Med. 2010;10:55.CrossRef
28.
Katrin S. Cytotoxicity of dietary flavonoids on different human cancer types. Pharmacogn Rev. 2014;8(16):122–46.CrossRef
29.
Rashid S, Rather MA, Shah WA, Bhat BA. Chemical composition, antimicrobial, cytotoxic and antioxidant activities of the essential oil of Artemisia indica Willd. Food Chem. 2013;138:693–700.CrossRefPubMed
30.
Harun FB, Jamalullail S, Yin K, Othman Z, Tilwari A, Balaram P. Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts from Eupatorium odoratum In Vitro: Effects on MCF-7 and Vero Cell Lines. Sci World J. 2012;2012:1–9.CrossRef
31.
Singh SA, Singh NR. Antimicrobial activity of Cassia didymobotrya and Phlogacanthus thyrsiformis. J Chem Pharmaceut Res. 2010;2(4):304–8.
32.
Mukherjee A, Chaliha M, Das S. Study of analgesic activityof ethanolextract of Phlogacanthus thyrsiformis on experimental animal models. Bangladesh J Pharmacol. 2009;4:147–9.CrossRef
33.
Chakravarty S, Kalita JC. Preliminary phytochemical screening and acute oral toxicity study of the flower of Phlogacanthus thyrsiformis Nees in albino mice. Int Res J Pharm. 2012;3(4):293–5.
34.
Pandey AK, Mohan M, Singh P, Palni UT, Tripathi NN. Chemical composition, antibacterial and antioxidant activity of essentialoil of Eupatorium adenophorum Spreng. From Eastern UttarPradesh, India. Food Biosci. 2014;7:80–7.CrossRef
35.
Taylor RSL, Manandhar NP, Hudson JB, Towers GHN. Antiviral activities of Nepalese medicinal plants. J Ethnopharmacol. 1996;52:157–63.CrossRefPubMed
36.
Chandrasekhar C, Prabhu KR, Venkateswarlu V. Isolation of a new quinone from Maesa macrophylla. Phytochemistry. 1970;9:415–7.CrossRef
37.
Boik J. Natural Compounds in Cancer Therapy. Minnesota, USA: Oregon Medical Press; 2001.
38.
39.
Johann S, Pizzolatti MG, Donnici CL, de Resende MA. Antifungal properties of plants used in brazilian traditional medicine against clinically relevant fungal pathogens. Braz J Microbiol. 2007;38:632–7.CrossRef
40.
Jain PK, Joshi H. Coumarin: Chemical and Pharmacological Profile. J Appl Pharmaceut Sci. 2012;2:236–40.
41.
Chiang C-T, Way T-D, Tsai S-J, Lin J-K. Diosgenin, a naturally occurring steroid, suppresses fatty acid synthase expression in HER2-overexpressing breast cancer cellsthrough modulating Akt, mTOR and JNK phosphorylation. FEBS Lett. 2007;581:5735–42.CrossRefPubMed
42.
Zhang S, Yang X, Morris ME. Flavonoids Are Inhibitors of Breast Cancer Resistance Protein (ABCG2)-Mediated Transport. Mol Pharmacol. 2004;65:1208–16.CrossRefPubMed
43.
Bishayee A, Ahmed S, Brankov N, Perloff M. Triterpenoids as potential agents for the chemoprevention and therapy of breast cancer. Front Biosci. 2011;16:980–96.CrossRef
44.
Thakur M, Melzig MF, Fuchs H, Weng A. Chemistry and pharmacology of saponins: special focus on cytotoxic properties. Botanics. 2011;1:19–29.
45.
Fong HH, Bhatti W, Farnsworth NR. Antitumor activity of certain plants due to tannins. J Pharm Sci. 1972;61:1818.CrossRefPubMed
Metadata
Title
The in vitro cytotoxic activity of ethno-pharmacological important plants of Darjeeling district of West Bengal against different human cancer cell lines
Authors
Bipransh K Tiwary
Sony Bihani
Anoop Kumar
Ranadhir Chakraborty
Runu Ghosh
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-015-0543-5

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