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BMC Oral Health
Published in: BMC Oral Health 1/2020

Open Access 01-12-2020 | Candida Balanitis | Research article

Effects of two peroxide enzymatic denture cleaners on Candida albicans biofilms and denture surface

Authors: Ying Han, Xiaodan Liu, Yu Cai

Published in: BMC Oral Health | Issue 1/2020

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Abstract

Objective

To compare the antifungal action of two commercially available denture cleaning agents to that of standard clinical solutions, and determine their effects on the polymethyl methacrylate (PMMA) acrylic resin denture surface.

Methods

Candida albicans growth was analyzed by colony forming assay, and the methyl thiazolyl tetrazolium (MTT) assay was used to evaluate biofilm formation and cell adhesion. The morphology and roughness of PMMA acrylic resin surface was measured by scanning electron microscopy (SEM) images and stylus method.

Results

Clene®, Polident® and 3% NaHCO3 solutions showed significantly greater antifungal effects in terms of both inhibiting growth and biofilm formation. In addition, Clene® solution prevented adhesion of C. albicans on cell culture plates compared to filter-sterile tap water, whereas other reagents did not have an inhibitory effect. One-month immersion in the different cleaning reagents significantly inhibited fungal adhesion on the PMMA surface Clene®, Polident® and 3% NaHCO3 showed greater effect compared to PBS and filter-sterile tap water. Finally, none of the cleansing agents significantly affected the morphology and roughness of the PMMA surface.

Conclusion

Clene®, Polident® and 3% NaHCO3 solutions can inhibit C. albicans growth and biofilm formation to some extent on cell culture plates, and significantly inhibit fungal adhesion on the PMMA surface without affecting surface morphology and roughness.
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Metadata
Title
Effects of two peroxide enzymatic denture cleaners on Candida albicans biofilms and denture surface
Authors
Ying Han
Xiaodan Liu
Yu Cai
Publication date
01-12-2020
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2020
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-020-01176-6

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