Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Oral Health
Published in: BMC Oral Health 1/2018

Open Access 01-12-2018 | Research article

Increased interleukin 1α and interleukin 1β expression is involved in the progression of periapical lesions in primary teeth

Authors: Ning-Yan Yang, Yan Zhou, Huan-Ying Zhao, Xiao-Yong Liu, Zheng Sun, Jia-Jian Shang

Published in: BMC Oral Health | Issue 1/2018

Login to get access

Abstract

Background

Interleukin 1 (IL-1) is involved in bone resorption. However, the role of IL-1 in periapical lesions characterized by periapical bone destruction in primary teeth has not yet been fully elucidated. This study aimed to detect the distribution and expression of IL-1 in periapical lesions in primary teeth and assess the relationship between the cytokines and the degree of inflammatory cell infiltration.

Methods

A total of 106 chronic periapical lesions in primary teeth were harvested. Haematoxylin and eosin (H&E) staining was used to determine the histological type and the inflammatory cell infiltration grade (mild, moderate, and severe), and immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the distribution and expression of IL-1α and IL-1β.

Results

Of the 106 chronic periapical lesion samples, there were 85 cases of periapical granuloma, accounting for 80.19% of the total samples, and 21 cases of radicular cysts, accounting for 19.81%; no cases of abscess were detected. Immunohistochemistry results showed that both IL-1α and IL-1β were expressed in periapical granulomas and cysts. ELISA results showed that IL-1α and IL-1β levels were higher in the periapical granuloma group than in the radicular cyst and normal control groups (P < 0.05). In the periapical granuloma group, IL-1α and IL-1β were detected at higher levels in the severe inflammatory cell infiltration subgroup than in the mild-inflammatory cell infiltration subgroup (P < 0.05), and IL-1β expression was also higher in the moderate inflammatory cell infiltration subgroup than in the mild inflammatory cell infiltration subgroup (P < 0.01). A significant positive correlation was observed between the protein expression levels of IL-1α and IL-1β and the inflammation grade in periapical granulomas from primary teeth (P < 0.05).

Conclusion

Expression levels of the cytokines IL-1α and IL-1β in periapical granulomas from primary teeth increased with increasing inflammatory severity and appeared to be a contributing factor to the progression of periapical lesions.
Literature
1.
Cordeiro MM, Rocha MJ. The effects of periradicular inflamation and infection on a primary tooth and permanent successor. J Clin Pediatr Dent. 2005;29:193–200.CrossRefPubMed
2.
Shang JJ, Yang QB, Zhao HY, Cai S, Zhou Y, Sun Z. Preliminary molecular analysis of bacterial composition in periapical lesions with primary endodontic infections of deciduous teeth. Chin Med J. 2013;126:3112–7.PubMed
3.
Silva MJ, Kajiya M, Alshwaimi E, Sasaki H, Hong J, Ok P, et al. Bacteria-reactive immune response may induce RANKL-expressing T cells in the mouse periapical bone loss lesion. J Endod. 2012;38:346–50.
4.
Bolan M, Lima DA, Figueiredo CP, Di Giunta G, Rocha MJ. Immunohistochemical study of presence of T cells, B cells, and macrophages in periradicular lesions of primary teeth. J Clin Pediatr Dent. 2008;32:287–93.CrossRefPubMed
5.
Schett G, Smolen JS. New insights in the mechanism of bone loss in arthritis. Curr Pharm Des. 2005;11:3039–49.CrossRefPubMed
6.
Ebersole JL, Kirakodu S, Novak MJ, Stromberg AJ, Shen S, Orraca L, et al. Cytokine gene expression profiles during initiation, progression and resolution of periodontitis. J Clin Periodontol. 2014;41: 853–61.
7.
Ng YL, Mann V, Rahbaran S, Lewsey J, Gulabivala K. Outcome of primary root canal treatment: systematic review of the literature -- part 2. Influence of clinical factors. Int Endod J. 2008;41:6–31.CrossRefPubMed
8.
Rosenwasser LJ. Biologic activities of IL-1 and its role in human disease. J Allergy Clin Immunol. 1998;102:344–50.CrossRefPubMed
9.
Martinez ZR, Naruishi K, Yamashiro K, Myokai F, Yamada T, Matsuura K, et al. Gene profiles during root canal treatment in experimental rat periapical lesions. J Endod. 2007;33:936–43.
10.
Tani-Ishii N, Wang CY, Stashenko P. Immunolocalization of bone-resorptive cytokines in rat pulp and periapical lesions following surgical pulp exposure. Oral Microbiol Immunol. 1995;10:213–9.CrossRefPubMed
11.
Martinho FC, Chiesa WM, Leite FR, Cirelli JA, Gomes BP. Correlation between clinical/radiographic features and inflammatory cytokine networks produced by macrophages stimulated with endodontic content. J Endod. 2012;38:740–5.CrossRefPubMed
12.
Morsani JM, Aminoshariae A, Han YW, Montagnese TA, Mickel A. Genetic predisposition to persistent apical periodontitis. J Endod. 2011;37:455–9.CrossRefPubMed
13.
Torabinejad M, Walton RE. Periradicular lesions. In: Ingle JI, Bakland LK, editors. Endodontics, 5th ed. Hamilton, Ont: BC Decker Ibnc; 2002. p. 175–201.
14.
Tsai CH, Weng SF, Yang LC, Huang FM, Chen YJ, Chang YC. Immunohistochemical localization of tissue-type plasminogen activator and type I plasminogen activator inhibitor in radicular cysts. J Oral Pathol Med. 2004;33:156–61.CrossRefPubMed
15.
Gazivoda D, Dzopalic T, Bozic B, Tatomirovic Z, Brkic Z, Colic M. Production of proinflammatory and immunoregulatory cytokines by inflammatory cells from periapical lesions in culture. J Oral Pathol Med. 2009;38:605–11.CrossRefPubMed
16.
Ramachandran Nair PN, Pajarola G, Schroeder HE. Types and incidence of human periapical lesions obtained with extracted teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996;81:93–102.CrossRefPubMed
17.
Gbadebo SO, Akinyamoju AO, Sulaiman AO. Periapical pathology: comparison of clinical diagnosis and histopathological findings. J West Afr Coll Surg. 2014;4:74–88.PubMedPubMedCentral
18.
Saraf PA, Kamat S, Puranik RS, Puranik S, Saraf SP, Singh BP. Comparative evaluation of immunohistochemistry, histopathology and conventional radiography in differentiating periapical lesions. J Conserv Dent. 2014;17:164–8.CrossRefPubMedPubMedCentral
19.
Safi L, Adl A, Azar MR, Akbary RA. Twenty-year survey of pathologic reports of two common types of chronic periapical lesions in shiraz dental school. J Dent Res Dent Clin Dent Prospects. 2008;2:63–70.PubMedPubMedCentral
20.
Mass E, Kaplan I, Hirshberg A. A clinical and histopathological study of radicular cysts associated with primary molars. J Oral Pathol Med. 1995;24:458–61.CrossRefPubMed
21.
Sullivan M, Gallagher G, Noonan V. The root of the problem: occurrence of typical and atypical periapical pathoses. J Am Dent Assoc. 2016;147:646–9.CrossRefPubMed
22.
Torabinejad M. Pulp and periradicular pathosis. In: Walton RE, Torabinejad M, editors. Principles and practice of endodontics, 3rd ed. Philadelphia: W.B. Sanders; 2002. p. 27–48.
23.
Fukushima A, Kajiya h IT, Shigeyama C, Okabe K, Anan H. Pro-inflammatory cytokines induce suppressor of cytokine signaling-3 in human periodontal ligament cells. J Endod. 2010;36:1004–8.CrossRefPubMed
24.
Matsumoto A, Anan H, Maeda K. An immunohistochemical study of the behavior of cells expressing interleukin-1 alpha and interleukin-1 beta within experimentally induced periapical lesions in rats. J Endod. 1998;24:811–6.CrossRefPubMed
25.
Motta AC, Furini RB, Simao JC, Ferreira MA, Komesu MC, Foss NT. The recurrence of leprosy reactional episodes could be associated with oral chronic infections and expression of serum IL-1, TNF-alpha, IL-6, IFN-gamma and IL-10. Braz Dent J. 2010;21:158–64.CrossRefPubMed
26.
Nair PN. Pathogenesis of apical periodontitis and the causes of endodontic failures. Crit Rev Oral Biol Med. 2004;15:348–81.CrossRefPubMed
27.
Andrade A, Santos EM, Carmo AF, Freitas RA, Galvão HC. Analysis of tryptase-positive mast cells and immunoexpression of MMP-9 and MMP-13 in periapical lesions. Int Endod J. 2017;50:446–54.CrossRefPubMed
28.
Yildirim S, Yapar M, Kubar A, Slots J. Human cytomegalovirus, Epstein-Barr virus and bone resorption-inducing cytokines in periapical lesions of deciduous teeth. Oral Microbiol Immunol. 2006;21:107–11.CrossRefPubMed
29.
Muglali M, Komerik N, Bulut E, Yarim GF, Celebi N, Sumer M. Cytokine and chemokine levels in radicular and residual cyst fluids. J Oral Pathol Med. 2008;37:185–9.CrossRefPubMed
30.
Trebec-Reynolds DP, Voronov I, Heersche JN, Manolson MF. IL-1 alpha and IL-1beta have different effects on formation and activity of large osteoclasts. J Cell Biochem. 2010;109:975–82.PubMed
31.
Jakovljevic A, Knezevic A, Karalic D, Soldatovic I, Popovic B, Milasin J, et al. Pro-inflammatory cytokine levels in human apical periodontitis: correlation with clinical and histological findings. Aust Endod J 2015; 41: 72–77.
32.
Rawlinson A, Dalati MH, Rahman S, Walsh TF, Fairclough AL. Interleukin-1 and IL-1 receptor antagonist in gingival crevicular fluid. J Clin Periodontol. 2000;27:738–43.CrossRefPubMed
33.
Bujak M, Frangogiannis NG. The role of IL-1 in the pathogenesis of heart disease. Arch Immunol Ther Exp. 2009;57:165–76.CrossRef
34.
Terlizzi M, Colarusso C, Popolo A, Pinto A, Sorrentino R. IL-1α and IL-1β-producing macrophages populate lung tumor lesions in mice. Oncotarget. 2016;7:58181–92.CrossRefPubMedPubMedCentral
35.
Metadata
Title
Increased interleukin 1α and interleukin 1β expression is involved in the progression of periapical lesions in primary teeth
Authors
Ning-Yan Yang
Yan Zhou
Huan-Ying Zhao
Xiao-Yong Liu
Zheng Sun
Jia-Jian Shang
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2018
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-018-0586-3

Other articles of this Issue 1/2018

BMC Oral Health 1/2018 Go to the issue

Research article

The effect of bitewing radiography on estimates of dental caries experience among children differs according to their disease experience

Research article

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing

Research article

A structural equation model to test a conceptual framework of oral health in Japanese edentulous patients with an item weighting method using factor score weights: a cross-sectional study

Research article

Evaluation of root resorption after comprehensive orthodontic treatment using cone beam computed tomography (CBCT): a meta-analysis

Case report

Extensive dental caries in a HIV positive adult patient on ART; case report and literature review

Research article

Effectiveness of an oral health intervention program for children with congenital heart defects