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BMC Oral Health
Published in: BMC Oral Health 1/2015

Open Access 01-12-2015 | Research article

Genotypic diversity and cariogenicity of Candida albicans from children with early childhood caries and caries-free children

Authors: Rongmin Qiu, Wenqing Li, Yan Lin, Dongsheng Yu, Wei Zhao

Published in: BMC Oral Health | Issue 1/2015

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Abstract

Background

The genotypic diversity and cariogenicity of C. albicans from the dental plaque of children are poorly understood. This study aimed to explore the genotypic diversity and cariogenicity of C. albicans from children with early childhood caries and caries-free children.

Methods

Dental plaque samples from 238 children with early childhood caries and from 125 caries-free children were collected for C. albicans isolation. A PCR method based on 25S rDNA was used to analyze C. albicans genotypes, and the strains with different genotypes were tested with regard to acidogenicity and aciduricity.

Results

Among 129 C. albicans isolates, 79 (61.2 %) belonged to genotype A. The distribution frequency of genotypes A and C or genotypes B and C showed no significant difference between children with early childhood caries and caries-free children (p = 0.178 and 0.148), whereas genotypes A and B exhibited significantly different distributions (p = 0.010). No significant differences in aciduricity were found among the three genotypes, but the acidogenicity of genotypes B and C differed significantly from that of genotype A at pH 4.0.

Conclusions

The genotypic distribution of C. albicans is associated with the caries experience of children, and the genotype may be related to its acidogenicity at pH 4.0.
Literature
1.
Dury TF, Horowitz AM, Ismail AI, Maertens MP, Rozier RG, Selwitz RH. Diagnosing and reporting early childhood caries for research purposes. A report of a workshop sponsored by the National Institute of Dental and Craniofacial Research, the Health Resources and Services Administration, and the Health Care Financing Administration. J Public Health Dent. 1999;59:192–7.CrossRef
2.
De Carvalho FG, Silva DS, Hebling J, Spolidorio LC, Spolidorio DMP. Presence of mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries. Arch Oral Biol. 2006;51:1024–8.CrossRefPubMed
3.
Raja M, Hannan A, Ali K. Association of oral candidal carriage with dental caries in children. Caries Res. 2010;44:272–6.CrossRefPubMed
4.
Qiu RM, Zhao W, Lin Y, Gao S, Luo W, Lu JX. Relationship between Candida albicans and early childhood caries [in Chinese]. Chin J Stomatol Res: Electron ED. 2010;4:284–7.
5.
Yang XQ, Zhang Q, Lu LY, Yang R, Liu Y, Zou J. Genotypic distribution of Candida albicans in dental biofilm of Chinese children associated with severe early childhood caries. Arch Oral Biol. 2012;57:1048–53.CrossRefPubMed
6.
Li WQ, Yu DS, Gao S, Lin JC, Chen ZY, Zhao W. Role of Candida albicans -secreted aspartyl proteinases (Saps) in severe early childhood caries. Int J Mol Sci. 2014;15:10766–79.CrossRefPubMedPubMedCentral
7.
Karahan ZC, Güriz H, Ağirbaşli H, Balaban N, Göçmen JS, Aysev D, et al. Genotype distribution of Candida albicans isolates by 25S intron analysis with regard to invasiveness. Mycoses. 2004;47:465–9.CrossRefPubMed
8.
Gurbuz M, Kaleli I. Molecular analysis of Candida albicans isolates from clinical specimens. Mycopathologia. 2010;169:261–7.CrossRefPubMed
9.
Liu XP, Fan SR, Bai FY, Li J, Liao QP. Antifungal susceptibility and genotypes of Candida albicans strains from patients with vulvovaginal candidiasis. Mycoses. 2009;52:24–8.CrossRefPubMed
10.
Zhao W, Qiu RM, Lin Y, Lu JX, Yu DS. Cariogenicity of Candida albicans isolated from children with early childhood caries and caries free [in Chinese]. Chin J Obstet Gynecol Pediatrics: Electron ED. 2011;7:295–8.
11.
Odds FC, Bernaerts R. CHROMagar Candida–a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol. 1994;32:1923–9.PubMedPubMedCentral
12.
13.
McCullogh MJ, Clemons KV, Stevens DA. Molecular and phenotypic characterization of genotypic C. albicans subgroups and comparison with Candida dubliniensis and Candida stellatoidea. J Clin Microbiol. 1999;37:417–21.
14.
Tamura M, Watanabe K, Mikami Y, Yazawa K, Nishimura K. Molecular characterization of new clinical isolates of Candida albicans and C. dubliniensis in Japan: analysis reveals a new genotype of C. albicans with group I intron. J Clin Microbiol. 2002;39:4309–15.CrossRef
15.
Qi QG, Hu T, Zou XD. Frequency, species and molecular characterization of oral Candida in hosts of different age in China. J Oral Pathol Med. 2005;34:352–6.CrossRefPubMed
16.
Nikawa H, Yamashiro H, Makihira S. In vitro cariogenic potential of Candida albicans. Mycoses. 2003;46:471–8.CrossRefPubMed
17.
Klinke T, Kneist S, de Soet JJ, Mauersberger S, Forster A, Klimm W. Acid production by oral strains of Candida albicans and lactobacilli. Caries Res. 2009;43:83–91.CrossRefPubMed
18.
Sen BH, Safavi KE, Spångberg LS. Colonization of C. albicans on cleaned human dental hard tissues. Arch Oral Biol. 1997;42:513–20.CrossRefPubMed
19.
Barros LM, Boriollo MF, Alves AC, Klein MI, Gonçalves RB, Höfling JF. Genetic diversity and exoenzyme activities of Candida albicans and Candida dubliniensis isolated from the oral cavity of Brazilian periodontal patients. Arch Oral Biol. 2008;53:1172–8.CrossRefPubMed
20.
Millar BC, Moore JE, Xu J, Walker MJ, Hedderwick S, McMullan R. Genotypic subgrouping of clinical isolates of Candida albicans and Candida dubliniensis by 25S intron analysis. Lett Appl Microbiol. 2002;35:102–6.CrossRefPubMed
Metadata
Title
Genotypic diversity and cariogenicity of Candida albicans from children with early childhood caries and caries-free children
Authors
Rongmin Qiu
Wenqing Li
Yan Lin
Dongsheng Yu
Wei Zhao
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2015
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-015-0134-3

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