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BMC Urology
Published in: BMC Urology 1/2019

Open Access 01-12-2019 | Fertility | Research article

Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry

Authors: Longlong Fu, Qi An, Kaishu Zhang, Ying Liu, Yue Tong, Jianfeng Xu, Fang Zhou, Xiaowei Wang, Ying Guo, Wenhong Lu, Xiaowei Liang, Yiqun Gu

Published in: BMC Urology | Issue 1/2019

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Abstract

Background

Human sperm cryopreservation is a simple and effective approach for male fertility preservation.

Methods

To identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy and highly reproducible proteomics, was used to quantitatively characterize the proteomics of human sperm cryopreservation.

Results

A total of 174 significantly differential proteins were identified between fresh and cryoperservated sperm: 98 proteins decreased and 76 proteins increased in the cryopreservation group. Bioinformatic analysis revealed that metabolic pathways play an important role in cryopreservation, including: propanoate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism. Four different proteins involved in glycolysis were identified by Western blotting: GPI, LDHB, ADH5, and PGAM1.

Conclusions

Our work will provide valuable information for future investigations and pathological studies involving sperm cryopreservation.
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Metadata
Title
Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry
Authors
Longlong Fu
Qi An
Kaishu Zhang
Ying Liu
Yue Tong
Jianfeng Xu
Fang Zhou
Xiaowei Wang
Ying Guo
Wenhong Lu
Xiaowei Liang
Yiqun Gu
Publication date
01-12-2019
Publisher
BioMed Central
Keyword
Fertility
Published in
BMC Urology / Issue 1/2019
Electronic ISSN: 1471-2490
DOI
https://doi.org/10.1186/s12894-019-0565-2

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