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Open Access 01-12-2019 | Hypospadia | Research article

The comparison of the effect of phenylephrine and epinephrine on penile spongy tissue in rats after experimental hypospadias surgery: a quantitative stereological study

Authors: Mehdi Shirazi, Mohammad-Bagher Rajabalian, Ali Noorafshan, Saied Karbalay-Doust, Zahra Jahanabadi, Faisal Ahmed

Published in: BMC Urology | Issue 1/2019

Abstract

Background

Many factors including vasoconstrictor agents can interfere with wound healing process. This study aimed to compare the histopathological outcome of injection of two sympathomimetic drugs used during urologic surgery, including phenylephrine and epinephrine, on the structure of spongy tissue and urethra in a rat model of experimental hypospadias repair using stereological methods.

Methods

Male rats were allocated into three groups. The first group underwent surgery without using any agents. The second and third groups underwent surgery with diluted phenylephrine (1:5000) and diluted epinephrine (1:100000) injection in the urethral plate before operation, respectively. Quantitative histological evaluation of all penises was performed after 3 weeks.

Results

The results indicated no significant differences among the three groups regarding the vessels and urethral lumen and epithelium. However, the volumes of the spongy tissue and collagen bundles and the number of fibroblasts were significantly higher (35–55%) in surgery + phenylephrine and surgery + epinephrine groups in comparison to the surgery group (p < 0.05), with no preferences.

Conclusions

Hypospadias repair using phenylephrine and epinephrine injection showed no adverse effects. Furthermore, they might lead to better postoperative structural outcomes without any preferences. However, further experimental and human studies are required to draw a firm conclusion.

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Title: The comparison of the effect of phenylephrine and epinephrine on penile spongy tissue in rats after experimental hypospadias surgery: a quantitative stereological study
Authors: Mehdi Shirazi
Mohammad-Bagher Rajabalian
Ali Noorafshan
Saied Karbalay-Doust
Zahra Jahanabadi
Faisal Ahmed
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Hypospadia
Published in: BMC Urology / Issue 1/2019
Electronic ISSN: 1471-2490
DOI: https://doi.org/10.1186/s12894-019-0483-3

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

The comparison of the effect of phenylephrine and epinephrine on penile spongy tissue in rats after experimental hypospadias surgery: a quantitative stereological study

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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