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Open Access 01-12-2018 | Research article

Comparison of mesenchymal stem cells obtained by suspended culture of synovium from patients with rheumatoid arthritis and osteoarthritis

Authors: Yuji Kohno, Mitsuru Mizuno, Nobutake Ozeki, Hisako Katano, Koji Otabe, Hideyuki Koga, Mikio Matsumoto, Haruka Kaneko, Yuji Takazawa, Ichiro Sekiya

Published in: BMC Musculoskeletal Disorders | Issue 1/2018

Abstract

Background

Mobilization of mesenchymal stem cells (MSCs) from the synovium was revealed using a “suspended synovium culture model” of osteoarthritis (OA). The pathology of rheumatoid arthritis (RA) differs from that of OA. We investigated whether mobilization of MSCs from the synovium also occurred in RA, and we compared the properties of synovial MSCs collected from suspended synovium culture models of RA and OA.

Methods

Human synovium was harvested during total knee arthroplasty from the knee joints of patients with RA (n = 8) and OA (n = 6). The synovium was suspended in a bottle containing culture medium and a culture dish at the bottom. Cells were harvested from the dish and analyzed.

Results

No significant difference was observed between RA and OA in the harvested cell numbers per g of synovium. However, the variation in the number of cells harvested from each donor was greater for RA than for OA. The harvested cells were multipotent and no difference was observed in the cartilage pellet weight between RA and OA. The surface epitopes of the cells in RA and OA were similar to those of MSCs.

Conclusion

Mobilization of MSCs from the synovium was demonstrated using a suspended synovium culture model for RA. The harvested cell numbers, chondrogenic potentials, and surface epitope profiles were comparable between the RA and OA models.

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Title: Comparison of mesenchymal stem cells obtained by suspended culture of synovium from patients with rheumatoid arthritis and osteoarthritis
Authors: Yuji Kohno
Mitsuru Mizuno
Nobutake Ozeki
Hisako Katano
Koji Otabe
Hideyuki Koga
Mikio Matsumoto
Haruka Kaneko
Yuji Takazawa
Ichiro Sekiya
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: BMC Musculoskeletal Disorders / Issue 1/2018
Electronic ISSN: 1471-2474
DOI: https://doi.org/10.1186/s12891-018-1998-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Comparison of mesenchymal stem cells obtained by suspended culture of synovium from patients with rheumatoid arthritis and osteoarthritis

Abstract

Background

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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