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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2017

Open Access 01-12-2017 | Research article

Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats

Authors: Ryusuke Saito, Takeshi Muneta, Nobutake Ozeki, Yusuke Nakagawa, Mio Udo, Katsuaki Yanagisawa, Kunikazu Tsuji, Makoto Tomita, Hideyuki Koga, Ichiro Sekiya

Published in: BMC Musculoskeletal Disorders | Issue 1/2017

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Abstract

Background

It is still debated whether strenuous running in the inflammatory phase produces beneficial or harmful effect in rat knees. We examined (1) the dropout rate of rats during a 30-km running protocol, (2) influences of strenuous running and/or low amounts of mono-iodoacetate injection on cartilage, and (3) the effect of strenuous running on synovitis.

Methods

Rats were forced to run 30 km over 6 weeks and the dropout rate was examined. One week after 0.1 mg mono-iodoacetate was injected into the right knee, rats were forced to run either 15 km or not run at all over 3 weeks, after which knee cartilage was evaluated. Synovium at the infrapatellar fat pad was also examined histologically.

Results

Even though all 12 rats run up to 15 km, only 6 rats completed 30 km of running. Macroscopically, 0.1 mg mono-iodoacetate induced erosion at the tibial cartilage irrespective of 15 km of running. Histologically, 0.1 mg mono-iodoacetate induced loss of cartilage matrix in the tibial cartilage, and an additional 15 km of strenuous running significantly exacerbated the loss. Synovitis caused by mono-iodoacetate improved after running.

Conclusions

Only 50% of rats completed 30 km of running because of foot problems. Strenuous running further exacerbated tibial cartilage erosion but did not influence synovitis induced by mono-iodoacetate.
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Metadata
Title
Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats
Authors
Ryusuke Saito
Takeshi Muneta
Nobutake Ozeki
Yusuke Nakagawa
Mio Udo
Katsuaki Yanagisawa
Kunikazu Tsuji
Makoto Tomita
Hideyuki Koga
Ichiro Sekiya
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2017
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-017-1393-8

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