Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2015

Open Access 01-12-2015 | Research article

Effects of prostaglandin E1 on callus formation in rabbits

Authors: Pawel V. Lipinsky, Ivan V. Sirotin, Alexandr V. Skoroglyadov, Alexey V. Ivkov, Alexandr P. Oettinger, Evgeny E. Krynetskiy, Alexandr B. But-Gusaim, Andreas J. Roth

Published in: BMC Musculoskeletal Disorders | Issue 1/2015

Login to get access

Abstract

Background

Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model.

Methods

Data were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. In all animals, the right tibia was fractured using a standardized protocol and stabilized by an intramedullary nail. Treatment group received a 5 μg/kg subcutaneous injection of PGE1/day during 10 postoperative days. Visual and radiological evaluation of callus formation was prospectively collected. After 30 days, all animals were killed and the tibia specimens were examined histologically.

Results

In all the treatment group animals, fractures were consolidated radiologically by day 30. No treatment group animals and two control group animals were excluded form the experiment. In the control group, 4 animals demonstrated slower callus formation than the main group. Two control group animals were excluded from the experiment on the 20th day due to wound infection; one developed a nonunion.
The mean coefficient of bone callus thickening in the main group was 2.08 (±0, 16) and 1.77 (±0.05) (p < 0.05) in the control group. Calculation of mean quantity of neogenic vessels in 10 random visual fields of the bone callus revealed 78 (±9.82) in the main group and 40 (±4.68) in the control group (p < 0.05).

Conclusions

Our study demonstrates an increased rate and amount of bone callus formation in the group treated with prostaglandin E1 compared to the control group. Prospective radiological analysis was corroborated by histologic evaluation.
Literature
1.
Hou S. Use of prostaglandin E1 (Promosan) in patients with replantation of extremities and vascularized free tissue transfer. Orth Surg. 1986;3:111–21.
2.
Maruyama Y, Nakajama H, Fujino Т, Koda E. The definition of cutaneous vascular territories over the black using selective angiography and the intraarterial injections of prostaglandin E1: some observations on the use of the lower trapezius myocutaneous flap. Br J Plast Surg. 1981;34:157–61.CrossRefPubMed
3.
Raisz LG, Martin TJ. Prostaglandins in bone and mineral metabolism. In: Peck WA, editor. Bone and Mineral Research, Annual 2. New York: Elsevier Science Publishers; 1984.
4.
Harvey W, Bennett A. Prostaglandins in bone resorption. Boca Raton: CRC Press; 1988.
5.
Dietrich JW, Goodson JM, Raisz LG. Stimulation of bone resorption by various prostaglandins in organ culture. Prostaglandins. 1975;10:231–40.CrossRefPubMed
6.
Ueda K, Saito A, Nakano H, Aoshima M, Yokota M, Muraoka R, et al. Cortical hyperostosis following long-term administration of prostaglandin E1 in infants with cyanotic congenital heart disease. J Pediat. 1980;97:834–6.CrossRefPubMed
7.
Sone K, Tashiro M, Fujinaga T, Tomomasa T, Tokuyama K, Kuroume T. Long-term low-dose prostaglandin E1 administration. J Pediatr. 1980;97:866–7.CrossRefPubMed
8.
Ringel RE, Brenner JI, Haney PJ, Burns JE, Moulton AL, Berman MA. Prostaglandin-induced periostitis: a complication of long-term PGE1 infusion in an infant with congenital heart disease. Radiology. 1982;142:657–8.CrossRefPubMed
9.
Lund JE, Brown WP, Treferman L. The toxicology of PGE1 and PGE2. In: Wu KK, Rossi CC, editors. Prostaglandins in clinical medicine: cardiovascular and thrombotic disorders. New York: Yearbook Medical Publishers; 1982. p. 93–103.
10.
Dodd DC, Port CD. Hyperostosis of the marrow cavity caused by misoprostol in CD-1 strain mice. Vet Pathol. 1987;24:545–8.PubMed
11.
Marks Jr SC, Miller SC. Local infusion of prostaglandin E1 stimulates mandibular bone formation in vivo. J Oral Path. 1988;17:500–5.CrossRefPubMed
12.
Shih MS, Norrdin RW. Effect of prostaglandin E1 on the periosteal regional acceleratory phenomenon in fractured ribs: histomorphometric study in beagles. Am J Vet Res. 1987;48:828–30.PubMed
13.
Shih MS, Norrdin RW. Effect of prostaglandin E2 on rib fracture healing in beagles: histomorphometric study on periosteum adjacent to the fracture site. Am J Vet Res. 1986;47:1561–4.PubMed
14.
Norrdin RW, Jee WS, High WB. The role of prostaglandins in bone in vivo. Prostaglandins Leukot Essent Fatty Acids. 1990;41:139–49.CrossRefPubMed
15.
Miller SC, Marks Jr SC. Local stimulation of new bone formation by prostaglandin E1: quantitative histomorphometry and comparison of delivery by minipumps and controlled-release pellets. Bone. 1993;14:143–51.CrossRefPubMed
16.
Сахно НВ. Стимуляция репаративных процессов костной ткани при переломах трубчатых костей у мелких домашних животных и профилактика хирургической инфекции. Диссертация, С.-Петербург, 2009. [Sakhno NV. Stimulation of reparative processes of bone fractures in the long bones from small animals and prevention of surgical infection. Thesis, St. Petersburg, 2009.]
17.
Ozturk AM, Cila E, Kanatli U, Isik I, Senkoylu A, Uzunok D, et al. Treatment of segmental bone defects in rats by the stimulation of bone marrow osteo-progenitor cells with prostaglandin E2. Int Orthop. 2005;29:73–7.CrossRefPubMedPubMedCentral
18.
Li M, Healy DR, Li Y, Simmons HA, Crawford DT, Ke HZ, et al. Osteopenia and impaired fracture healing in aged EP4 receptor knockout mice. Bone. 2005;37:46–54.CrossRefPubMed
19.
Hommann M, Kämmerer D, Lehmann G, Kornberg A, Küpper B, Daffner W, et al. Prevention of early loss of bone mineral density after liver transplantation by prostaglandin E1. Transplant Proc. 2007;39:540–3.CrossRefPubMed
20.
Shih M, Norrdin R. Effect of prostaglandin E1 on regional haversian remodeling in beagles with fractured ribs: a histomorphometric study. Bone Miner. 1987;8:87–90.CrossRef
21.
Щербавская ЭА, Гельцер Б И. Состояние костной ткани у беременных с гестозом. Российский вестник акушера-гинеколога, №.1 2003. Медиа Сфера. [Sherbavskaya EA Geltser B I. Composition of bone in women with preeclampsia. Russian Gazette obstetrician-gynecologist, №.1. 2003. Media Sphere.]
22.
Риггз Б. Л., Мелтон III Л. Дж. Остеопороз. Этиология, диагностика, лечение, «Бином», Санкт-Петербург 2000.- 558 с. [Riggs BL, Melton JL. Osteoporosis. Etiology, diagnosis, and management. Russian edition. M. BINOM St. Peterburg. 2000; 558.]
23.
Marie P. Growth factors and bone formation in osteoporosis: role for IGF-I and TGF beta. Rev Rhum Engl Ed. 1997;64:44–53.PubMed
24.
High WB, Brusse DM, Factor S. Effects of prostaglandin E2 on fracture healing in adult dogs. J Bone Min Res. 1989;4:S404.
25.
Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, et al. Bone histomorphometry:standardization of nomenclature, symbols, and units. Report of the ASBMR histomorphometry nomenclature committee. J Bone Miner Res. 1987;2:595–610.CrossRefPubMed
26.
Arosarena OA, Collins WL. Defect repair in the rat mandible with bone morphogenic protein 5 and prostaglandin E1. Arch Otolaryngol Head Neck Surg. 2003;129:1125–30.CrossRefPubMed
Metadata
Title
Effects of prostaglandin E1 on callus formation in rabbits
Authors
Pawel V. Lipinsky
Ivan V. Sirotin
Alexandr V. Skoroglyadov
Alexey V. Ivkov
Alexandr P. Oettinger
Evgeny E. Krynetskiy
Alexandr B. But-Gusaim
Andreas J. Roth
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2015
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-015-0695-y

Other articles of this Issue 1/2015

BMC Musculoskeletal Disorders 1/2015 Go to the issue

Case report

Recurrent episodes of micturition with expulsion of symphyseal plate screws following pelvic ring fixation: case report

Study protocol

A multi-centre randomized controlled trial comparing arthroscopic osteochondroplasty and lavage with arthroscopic lavage alone on patient important outcomes and quality of life in the treatment of young adult (18–50) Femoroacetabular impingement

Research article

Can a physical activity monitor provide a valid measure of arm elevation angle? A study to assess agreement between the SenseWear Mini Armband and the universal goniometer

Research article

Screening for IgG4-type anti-nuclear antibodies in IgG4-related disease

Research article

Short-term effect on pain and function of neurophysiological education and sensorimotor retraining compared to usual physiotherapy in patients with chronic or recurrent non-specific low back pain, a pilot randomized controlled trial

Case report

Adolescent idiopathic scoliosis without limb weakness: a differential diagnosis of core myopathy?