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BMC Ophthalmology
Published in: BMC Ophthalmology 1/2017

Open Access 01-12-2017 | Research article

The Antibiofilm efficacy of nitric oxide on soft contact lenses

Authors: Dong Ju Kim, Joo-Hee Park, Marth Kim, Choul Yong Park

Published in: BMC Ophthalmology | Issue 1/2017

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Abstract

Background

To investigate the antibiofilm efficacy of nitric oxide (NO) on soft contact lenses.

Methods

Nitrite (NO precursor) release from various concentrations (0–1000 μM) of sodium nitrite (NaNO2, NO donor) was measured by Griess Assay. Cell viability assay was performed using human corneal epithelial cell under various concentration (0–1000 μM) of NaNO2. Biofilm formation on soft contact lenses was achieved by adding Staphylococcus aureus or Pseudomonas aeruginosa to the culture media. Various concentrations of NaNO2 (0–1000 μM) were added to the culture media, each containing soft contact lens. After incubation in NaNO2 containing culture media for 1, 3, or 7 days, each contact lens was transferred to a fresh, bacteria-free media without NaNO2. The bacteria in the biofilm were dispersed in the culture media for planktonic growth. After reculturing the lenses in the fresh media for 24 h, optical density (OD) of media was measured at 600 nm and colony forming unit (CFU) was counted by spreading media on tryptic soy agar plate for additional 18 h.

Results

Nitrite release from NaNO2 showed dose-dependent suppressive effect on biofilm formation. Most nitrite release from NaNO2 tended to occur within 30 min. The viability of human corneal epithelial cells was well maintained at tested NaNO2 concentrations. The bacterial CFU and OD showed dose-dependent decrease in the NaNO2 treated samples on days 1, 3 and 7 for both Staphylococcus aureus and Pseudomonas aeruginosa.

Conclusions

NO successfully inhibited the biofilm formation by Staphylococcus aureus or Pseudomonas aeruginosa on soft contact lenses in dose-dependent manner.
Literature
1.
Dart JK, Stapleton F, Minassian D. Contact lenses and other risk factors in microbial keratitis. Lancet. 1991;338(8768):650–3.CrossRefPubMed
2.
3.
Morgan PB, Efron N, Hill EA, Raynor MK, Whiting MA, Tullo AB. Incidence of keratitis of varying severity among contact lens wearers. Br J Ophthalmol. 2005;89(4):430–6.CrossRefPubMedPubMedCentral
4.
Robertson DM. The effects of silicone hydrogel lens wear on the corneal epithelium and risk for microbial keratitis. Eye Contact Lens. 2013;39(1):67–72.CrossRefPubMedPubMedCentral
5.
Stern GA. Contact lens associated bacterial keratitis: past, present, and future. CLAO J. 1998;24(1):52–6.PubMed
6.
McLaughlin-Borlace L, Stapleton F, Matheson M, Dart JK. Bacterial biofilm on contact lenses and lens storage cases in wearers with microbial keratitis. J Appl Microbiol. 1998;84(5):827–38.CrossRefPubMed
7.
Percival SL, Suleman L, Vuotto C, Donelli G. Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control. J Med Microbiol. 2015;64(Pt 4):323–34.CrossRefPubMed
8.
Veerachamy S, Yarlagadda T, Manivasagam G, Yarlagadda PK. Bacterial adherence and biofilm formation on medical implants: a review. Proc Inst Mech Eng H J Eng Med. 2014;228(10):1083–99.CrossRef
9.
10.
Cheng KH, Leung SL, Hoekman HW, Beekhuis WH, Mulder PG, Geerards AJ, Kijlstra A. Incidence of contact-lens-associated microbial keratitis and its related morbidity. Lancet. 1999;354(9174):181–5.CrossRefPubMed
11.
MacMicking J, Xie QW, Nathan C. Nitric oxide and macrophage function. Annu Rev Immunol. 1997;15:323–50.CrossRefPubMed
12.
13.
Barraud N, Hassett DJ, Hwang SH, Rice SA, Kjelleberg S, Webb JS. Involvement of nitric oxide in biofilm dispersal of Pseudomonas Aeruginosa. J Bacteriol. 2006;188(21):7344–53.CrossRefPubMedPubMedCentral
14.
15.
Barraud N, Kelso MJ, Rice SA, Kjelleberg S. Nitric oxide: a key mediator of biofilm dispersal with applications in infectious diseases. Curr Pharm Des. 2015;21(1):31–42.CrossRefPubMed
16.
Arora DP, Hossain S, Xu Y, Boon EM. Nitric oxide regulation of bacterial biofilms. Biochemistry. 2015;54(24):3717–28.CrossRefPubMed
17.
Barraud N, Storey MV, Moore ZP, Webb JS, Rice SA, Kjelleberg S. Nitric oxide-mediated dispersal in single- and multi-species biofilms of clinically and industrially relevant microorganisms. Microb Biotechnol. 2009;2(3):370–8.CrossRefPubMedPubMedCentral
18.
19.
20.
21.
Schlag S, Fuchs S, Nerz C, Gaupp R, Engelmann S, Liebeke M, Lalk M, Hecker M, Gotz F. Characterization of the oxygen-responsive NreABC regulon of Staphylococcus Aureus. J Bacteriol. 2008;190(23):7847–58.CrossRefPubMedPubMedCentral
22.
Robinson JL, Jaslove JM, Murawski AM, Fazen CH, Brynildsen MP. An integrated network analysis reveals that nitric oxide reductase prevents metabolic cycling of nitric oxide by Pseudomonas Aeruginosa. Metab Eng. 2017;41:67–81.CrossRefPubMed
23.
Passos RM, Cariello AJ, MC Y, Hofling-Lima AL. Microbial keratitis in the elderly: a 32-year review. Arq Bras Oftalmol. 2010;73(4):315–9.CrossRefPubMed
24.
Fleiszig SM, Evans DJ. The pathogenesis of bacterial keratitis: studies with Pseudomonas Aeruginosa. Clin Exp Optom. 2002;85(5):271–8.CrossRefPubMed
25.
Francolini I, Donelli G. Prevention and control of biofilm-based medical-device-related infections. FEMS Immunol Med Microbiol. 2010;59(3):227–38.CrossRefPubMed
26.
Hoddenbach JG, Boekhoorn SS, Wubbels R, Vreugdenhil W, Van Rooij J, Geerards AJ. Clinical presentation and morbidity of contact lens-associated microbial keratitis: a retrospective study. Graefes Arch Clin Exp Ophthalmol. 2014;252(2):299–306.CrossRefPubMed
27.
Bourcier T, Thomas F, Borderie V, Chaumeil C, Laroche L. Bacterial keratitis: predisposing factors, clinical and microbiological review of 300 cases. Br J Ophthalmol. 2003;87(7):834–8.CrossRefPubMedPubMedCentral
28.
Keay L, Edwards K, Naduvilath T, Taylor HR, Snibson GR, Forde K, Stapleton F. Microbial keratitis predisposing factors and morbidity. Ophthalmology. 2006;113(1):109–16.CrossRefPubMed
29.
Bruinsma GM, van der Mei HC, Busscher HJ. Bacterial adhesion to surface hydrophilic and hydrophobic contact lenses. Biomaterials. 2001;22(24):3217–24.CrossRefPubMed
30.
Liesegang TJ. Contact lens-related microbial keratitis: part II: Pathophysiology. Cornea. 1997;16(3):265–73.CrossRefPubMed
31.
Tam C, Mun JJ, Evans DJ, Fleiszig SM. The impact of inoculation parameters on the pathogenesis of contact lens-related infectious keratitis. Invest Ophthalmol Vis Sci. 2010;51(6):3100–6.CrossRefPubMedPubMedCentral
32.
Anwar H, Dasgupta MK, Costerton JW. Testing the susceptibility of bacteria in biofilms to antibacterial agents. Antimicrob Agents Chemother. 1990;34(11):2043–6.CrossRefPubMedPubMedCentral
33.
Cutruzzola F, Frankenberg-Dinkel N. Origin and impact of nitric oxide in Pseudomonas Aeruginosa biofilms. J Bacteriol. 2016;198(1):55–65.CrossRefPubMed
34.
Schlag S, Nerz C, Birkenstock TA, Altenberend F, Gotz F. Inhibition of staphylococcal biofilm formation by nitrite. J Bacteriol. 2007;189(21):7911–9.CrossRefPubMedPubMedCentral
35.
Jenal U, Malone J. Mechanisms of cyclic-di-GMP signaling in bacteria. Annu Rev Genet. 2006;40:385–407.CrossRefPubMed
36.
Liu N, Xu Y, Hossain S, Huang N, Coursolle D, Gralnick JA, Boon EM. Nitric oxide regulation of cyclic di-GMP synthesis and hydrolysis in Shewanella woodyi. Biochemistry. 2012;51(10):2087–99.CrossRefPubMed
37.
Barraud N, Kardak BG, Yepuri NR, Howlin RP, Webb JS, Faust SN, Kjelleberg S, Rice SA, Kelso MJ. Cephalosporin-3′-diazeniumdiolates: targeted NO-donor prodrugs for dispersing bacterial biofilms. Angew Chem Int Ed Eng. 2012;51(36):9057–60.CrossRef
38.
Friedman A, Blecher K, Sanchez D, Tuckman-Vernon C, Gialanella P, Friedman JM, Martinez LR, Nosanchuk JD. Susceptibility of gram-positive and -negative bacteria to novel nitric oxide-releasing nanoparticle technology. Virulence. 2011;2(3):217–21.CrossRefPubMed
39.
Jones ML, Ganopolsky JG, Labbe A, Wahl C, Prakash S. Antimicrobial properties of nitric oxide and its application in antimicrobial formulations and medical devices. Appl Microbiol Biotechnol. 2010;88(2):401–7.CrossRefPubMed
40.
Miller C, McMullin B, Ghaffari A, Stenzler A, Pick N, Roscoe D, Ghahary A, Road J, Av-Gay Y. Gaseous nitric oxide bactericidal activity retained during intermittent high-dose short duration exposure. Nitric Oxide. 2009;20(1):16–23.CrossRefPubMed
41.
Allan RN, Morgan S, Brito-Mutunayagam S, Skipp P, Feelisch M, Hayes SM, Hellier W, Clarke SC, Stoodley P, Burgess A, et al. Low concentrations of nitric oxide modulate Streptococcus Pneumoniae biofilm metabolism and antibiotic tolerance. Antimicrob Agents Chemother. 2016;60(4):2456–66.CrossRefPubMedPubMedCentral
42.
Major TA, Panmanee W, Mortensen JE, Gray LD, Hoglen N, Hassett DJ. Sodium nitrite-mediated killing of the major cystic fibrosis pathogens Pseudomonas Aeruginosa, Staphylococcus Aureus, and Burkholderia cepacia under anaerobic planktonic and biofilm conditions. Antimicrob Agents Chemother. 2010;54(11):4671–7.CrossRefPubMedPubMedCentral
43.
Jardeleza C, Foreman A, Baker L, Paramasivan S, Field J, Tan LW, Wormald PJ. The effects of nitric oxide on Staphylococcus Aureus biofilm growth and its implications in chronic rhinosinusitis. Int Forum Allergy Rhinol. 2011;1(6):438–44.CrossRefPubMed
44.
Okajima Y, Kobayakawa S, Tsuji A, Tochikubo T. Biofilm formation by Staphylococcus Epidermidis on intraocular lens material. Invest Ophthalmol Vis Sci. 2006;47(7):2971–5.CrossRefPubMed
45.
Baillif S, Casoli E, Marion K, Roques C, Pellon G, Hartmann DJ, Freney J, Burillon C, Kodjikian L. A novel in vitro model to study staphylococcal biofilm formation on intraocular lenses under hydrodynamic conditions. Invest Ophthalmol Vis Sci. 2006;47(8):3410–6.CrossRefPubMed
46.
YT W, Tam C, Zhu LS, Evans DJ, Fleiszig SM. Human tear fluid reduces Culturability of contact lens-associated Pseudomonas Aeruginosa biofilms but induces expression of the virulence-associated type III secretion system. Ocul Surf. 2017;15(1):88–96.CrossRef
47.
Bebarta VS, Brittain M, Chan A, Garrett N, Yoon D, Burney T, Mukai D, Babin M, Pilz RB, Mahon SB, et al. Sodium nitrite and sodium Thiosulfate are effective against acute cyanide poisoning when administered by intramuscular injection. Ann Emerg Med. 2017;69(6):718–25. e714CrossRefPubMed
48.
Ulici A, Jancik J, Lam TS, Reidt S, Calcaterra D, Cole JB. Clevidipine versus sodium nitroprusside in acute aortic dissection: a retrospective chart review. Am J Emerg Med. 2017;
Metadata
Title
The Antibiofilm efficacy of nitric oxide on soft contact lenses
Authors
Dong Ju Kim
Joo-Hee Park
Marth Kim
Choul Yong Park
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Ophthalmology / Issue 1/2017
Electronic ISSN: 1471-2415
DOI
https://doi.org/10.1186/s12886-017-0604-2

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