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BMC Ophthalmology
Published in: BMC Ophthalmology 1/2016

Open Access 01-12-2016 | Research article

Comparison of human corneal cell density by age and corneal location: an in vivo confocal microscopy study

Authors: Tianyu Zheng, Qihua Le, Jiaxu Hong, Jianjiang Xu

Published in: BMC Ophthalmology | Issue 1/2016

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Abstract

Background

Peripheral and central regions of the cornea are optically different and have different repair capacity and pathology. For this reason, we characterized the cellular morphology and quantified the cell density of the central and peripheral regions of the cornea with age.

Methods

Eighty healthy subjects were enrolled in the study and divided into four groups according to age: A (0–19 years), B (20–39 years), C (40–59 years), and D (>60 years). In vivo confocal microscopy was used to measure the following parameters for the central and peripheral regions of the cornea: average cellular density and area of the superficial and basal epithelium; average density of the anterior and posterior keratocytes; average endothelial cell density and cellular area; percentage of hexagonal endothelial cells.

Results

Statistically significant differences between the central and peripheral cornea were observed for the cellular density of basal epithelial cells in group A. The density of keratocytes in the anterior stroma was significantly greater in the central region compared with the peripheral region in group B and group C. The percentage of hexagonal cells in the endothelial layer was significantly greater in the central region compared with the peripheral region. Age-related changes were found in peripheral basal epithelial cell density, central and peripheral endothelial cell density, and the percentage of hexagonal endothelial cells.

Conclusion

Both similarities and differences in morphology of the central and peripheral regions of the transparent cornea were observed. These observations would provide a histological basis for further studies to define its regional pathological mechanisms.
Literature
1.
2.
Maurice DM, Watson PG. The distribution and movement of serum albumin in the cornea. Experimental eye research. 1965;4(4):355–63.CrossRefPubMed
3.
Gipson IK, Hori Y, Argueso P. Character of ocular surface mucins and their alteration in dry eye disease. The ocular surface. 2004;2(2):131–48.CrossRefPubMed
4.
Muller LJ, Vrensen GF, Pels L, Cardozo BN, Willekens B. Architecture of human corneal nerves. Investigative ophthalmology & visual science. 1997;38(5):985–94.
5.
Muller LJ, Pels E, Schurmans LR, Vrensen GF. A new three-dimensional model of the organization of proteoglycans and collagen fibrils in the human corneal stroma. Experimental eye research. 2004;78(3):493–501.CrossRefPubMed
6.
Lavker RM, Dong G, Cheng SZ, Kudoh K, Cotsarelis G, Sun TT. Relative proliferative rates of limbal and corneal epithelia. Implications of corneal epithelial migration, circadian rhythm, and suprabasally located DNA-synthesizing keratinocytes. Investigative ophthalmology & visual science. 1991;32(6):1864–75.
7.
Cotsarelis G, Cheng SZ, Dong G, Sun TT, Lavker RM. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell. 1989;57(2):201–9.CrossRefPubMed
8.
Stickel TE, Bonanno JA. The relationship between corneal oxygen tension and hypoxic corneal edema. Optometry. 2002;73(10):598–604.PubMed
9.
10.
Kaufman SC, Musch DC, Belin MW, Cohen EJ, Meisler DM, Reinhart WJ, Udell IJ, Van Meter WS. Confocal microscopy: a report by the American Academy of Ophthalmology. Ophthalmology. 2004;111(2):396–406.CrossRefPubMed
11.
Niederer RL, Perumal D, Sherwin T, McGhee CN. Age-related differences in the normal human cornea: a laser scanning in vivo confocal microscopy study. The British journal of ophthalmology. 2007;91(9):1165–9.CrossRefPubMedPubMedCentral
12.
Berlau J, Becker HH, Stave J, Oriwol C, Guthoff RF. Depth and age-dependent distribution of keratocytes in healthy human corneas: a study using scanning-slit confocal microscopy in vivo. Journal of cataract and refractive surgery. 2002;28(4):611–6.CrossRefPubMed
13.
Vanathi M, Tandon R, Sharma N, Titiyal JS, Pandey RM, Vajpayee RB. In-vivo slit scanning confocal microscopy of normal corneas in Indian eyes. Indian journal of ophthalmology. 2003;51(3):225–30.PubMed
14.
Yee RW, Matsuda M, Schultz RO, Edelhauser HF. Changes in the normal corneal endothelial cellular pattern as a function of age. Current eye research. 1985;4(6):671–8.CrossRefPubMed
15.
Oguz H, Yokoi N, Kinoshita S. The height and radius of the tear meniscus and methods for examining these parameters. Cornea. 2000;19(4):497–500.CrossRefPubMed
16.
Mustonen RK, McDonald MB, Srivannaboon S, Tan AL, Doubrava MW, Kim CK. Normal human corneal cell populations evaluated by in vivo scanning slit confocal microscopy. Cornea. 1998;17(5):485–92.CrossRefPubMed
17.
Fuchs E, Dowling J, Segre J, Lo SH, Yu QC. Integrators of epidermal growth and differentiation: distinct functions for beta 1 and beta 4 integrins. Curr Opin Genet Dev. 1997;7(5):672–82.CrossRefPubMed
18.
Kruse FE. Stem cells and corneal epithelial regeneration. Eye (Lond). 1994;8(Pt 2):170–83.CrossRef
19.
Xu KP, Zoukhri D, Zieske JD, Dartt DA, Sergheraert C, Loing E, Yu FS. A role for MAP kinase in regulating ectodomain shedding of APLP2 in corneal epithelial cells. Am J Physiol Cell Physiol. 2001;281(2):C603–614.PubMed
20.
Zheng T, Xu J. Age-related changes of human limbus on in vivo confocal microscopy. Cornea. 2008;27(7):782–6.CrossRefPubMed
21.
Furstenberger G, Gross M, Schweizer J, Vogt I, Marks F. Isolation, characterization and in vitro cultivation of subfractions of neonatal mouse keratinocytes: effects of phorbol esters. Carcinogenesis. 1986;7(10):1745–53.CrossRefPubMed
22.
Gross M, Furstenberger G, Marks F. Isolation, characterization, and in vitro cultivation of keratinocyte subfractions from adult NMRI mouse epidermis: epidermal target cells for phorbol esters. Exp Cell Res. 1987;171(2):460–74.CrossRefPubMed
23.
24.
Kobayashi A, Sugiyama K. In vivo corneal confocal microscopic findings of palisades of Vogt and its underlying limbal stroma. Cornea. 2005;24(4):435–7.CrossRefPubMed
25.
Romano AC, Espana EM, Yoo SH, Budak MT, Wolosin JM, Tseng SC. Different cell sizes in human limbal and central corneal basal epithelia measured by confocal microscopy and flow cytometry. Invest Ophthalmol Vis Sci. 2003;44(12):5125–9.CrossRefPubMed
26.
Kamma-Lorger CS, Boote C, Hayes S, Moger J, Burghammer M, Knupp C, Quantock AJ, Sorensen T, Di Cola E, White N, et al. Collagen and mature elastic fibre organisation as a function of depth in the human cornea and limbus. J Struct Biol. 2010;169(3):424–30.CrossRefPubMed
27.
Abahussin M, Hayes S, Knox Cartwright NE, Kamma-Lorger CS, Khan Y, Marshall J, Meek KM. 3D collagen orientation study of the human cornea using X-ray diffraction and femtosecond laser technology. Invest Ophthalmol Vis Sci. 2009;50(11):5159–64.CrossRefPubMed
28.
Meek KM, Boote C. The organization of collagen in the corneal stroma. Exp Eye Res. 2004;78(3):503–12.CrossRefPubMed
29.
Hamada R, Giraud JP, Graf B, Pouliquen Y. Analytical and statistical study of the lamellae, keratocytes and collagen fibrils of the central region of the normal human cornea. (Light and electron microscopy). Arch Ophtalmol Rev Gen Ophtalmol. 1972;32(8):563–70.PubMed
30.
Aghamohammadzadeh H, Newton RH, Meek KM. X-ray scattering used to map the preferred collagen orientation in the human cornea and limbus. Structure. 2004;12(2):249–56.CrossRefPubMed
31.
Wilson SE, Mohan RR, Hutcheon AE, Mohan RR, Ambrosio R, Zieske JD, Hong J, Lee J. Effect of ectopic epithelial tissue within the stroma on keratocyte apoptosis, mitosis, and myofibroblast transformation. Exp Eye Res. 2003;76(2):193–201.CrossRefPubMed
32.
Wilson SE, Mohan RR, Mohan RR, Ambrosio Jr R, Hong J, Lee J. The corneal wound healing response: cytokine-mediated interaction of the epithelium, stroma, and inflammatory cells. Prog Retin Eye Res. 2001;20(5):625–37.CrossRefPubMed
33.
Wilson SE. Molecular cell biology for the refractive corneal surgeon: programmed cell death and wound healing. J Refract Surg. 1997;13(2):171–5.PubMed
34.
Foster DS, Azar DT, Dohlman CH. Smolin and Thoft’s The Cornea: scientific foundations and clinical practice. 4th ed. Philadelphia, PA: Lippincott Williams & Wilkins; 2004. p. 48.
Metadata
Title
Comparison of human corneal cell density by age and corneal location: an in vivo confocal microscopy study
Authors
Tianyu Zheng
Qihua Le
Jiaxu Hong
Jianjiang Xu
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Ophthalmology / Issue 1/2016
Electronic ISSN: 1471-2415
DOI
https://doi.org/10.1186/s12886-016-0290-5

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