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BMC Ophthalmology
Published in: BMC Ophthalmology 1/2016

Open Access 01-12-2016 | Research article

Iris ultrastructure in patients with synechiae as revealed by in vivo laser scanning confocal microscopy

In vivo iris ultrastructure in patients with Synechiae by Laser Scanning Confocal Microscopy

Authors: Ming Li, Hongbo Cheng, Ping Guo, Chun Zhang, Song Tang, Shusheng Wang

Published in: BMC Ophthalmology | Issue 1/2016

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Abstract

Background

Iris plays important roles in ocular physiology and disease pathogenesis. Currently it is technically challenging to noninvasively examine the human iris ultrastructure in vivo. The purpose of the current study is to reveal human iris ultrastructure in patients with synechiae by using noninvasive in vivo laser scanning confocal microscopy (LSCM).

Methods

The ultrastructure of iris in thirty one patients, each with synechiae but transparent cornea, was examined by in vivo LSCM.

Results

Five characteristic iris ultrastructures was revealed in patients with synechiae by in vivo LSCM, which include: 1. tree trunk-like structure; 2. tree branch/bush-like structure; 3. Fruit-like structure; 4. Epithelioid-like structure; 5. deep structure. Pigment granules can be observed as a loose structure on the top of the arborization structure. In iris-associated diseases with Tyndall’s Phenomenon and keratic precipitates, the pigment particles are more likely to fall off from the arborization structure.

Conclusions

The ultrastructure of iris in patients with synechiae has been visualized using in vivo LSCM. Five iris ultrastructures can be clearly observed, with some of the structures maybe disease-associated. The fall-off of the pigment particles may cause the Tyndall’s Phenomenon positive. In vivo LSCM provides a non-invasive approach to observe the human iris ultrastructure under certain eye disease conditions, which sets up a foundation to visualize certain iris-associated diseases in the future.
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Literature
1.
Gritz DC, Wong IG. Incidence and prevalence of uveitis in Northern California: the Northern California Epidemiology of Uveitis Study. Ophthalmology. 2004;111(3):491–500. PubMed: 15019324.CrossRefPubMed
2.
Sbeity Z, Palmiero PM, Tello C, et al. Noncontact in vivo confocal laser scanning microscopy of exfoliation syndrome. Trans Am Ophthalmol Soc. 2008;106(3):46–55. PubMed: 19277220.PubMedPubMedCentral
3.
Tavakoli M, Hossain P, Malik RA. Clinical applications of corneal confocal microscopy. Clin Ophthalmol. 2008;2(2):435–45. PubMed: 19668734.PubMedPubMedCentral
4.
Kaufman SC, Beuerman RW, Kaufman HE. Diagnosis of advanced Fuchs’ endothelial dystrophy with the confocal microscope. Am J Ophthalmol. 1993;116(5):652–3. PubMed: 8238235.CrossRefPubMed
5.
Chiou AG, Kaufman SC, Beuerman RW, Ohta T, et al. Confocal microscopy in the iridocorneal endothelial syndrome. Br J Ophthalmol. 1999;83(6):697–702. PubMed: 10340979.CrossRefPubMedPubMedCentral
6.
Chiou AG, Cadez R, Böhnke M. Diagnosis of Dieffenbachia induced corneal injury by confocal microscopy. Br J Ophthalmol. 1997;81(2):168–9. PubMed: 9059255.CrossRefPubMedPubMedCentral
7.
Kaufman SC, Chew SJ, Capps SC, et al. Confocal microscopy of corneal penetration by tarantula hairs. Scanning. 1994;16(5):312–5. PubMed: 7994494.CrossRefPubMed
8.
Cavanagh HD, McCulley JP. In vivo confocal microscopy and acanthamoeba keratitis. Am J Ophthalmol. 1996;121(2):207–8. PubMed: 8623891.CrossRefPubMed
9.
Chew SJ, Beuerman RW, Assouline M, et al. Early diagnosis of infectious keratitis with in vivo real time confocal microscopy. CLAO J. 1992;18(3):197–201. PubMed: 1499129.PubMed
10.
Florakis GJ, Moazami G, Schubert H, et al. Scanning slit confocal microscopy of fungal keratitis. Arch Ophthalmol. 1997;115(11):1461–3. PubMed: 9366681.CrossRefPubMed
11.
Pfister DR, Cameron JD, Krachmer JH, et al. Confocal microscopy findings of acanthamoeba keratitis. Am J Ophthalmol. 1996;121(2):119–28. PubMed: 8623881.CrossRefPubMed
12.
Shah GK, Pfister D, Probst LE, et al. Diagnosis of microsporidial keratitis by confocal microscopy and the chromatrope stain. Am J Ophthalmol. 1996;121(1):89–91. PubMed: 8554085.CrossRefPubMed
13.
Sutphin JE, Kantor AL, Mathers WD, et al. Evaluation of infectious crystalline keratitis with confocal microscopy in a case series. Cornea. 1997;16(1):21–6. PubMed: 8985629.CrossRefPubMed
14.
Sbeity Z, Palmiero PM, Tello C, et al. Non-contact in vivo confocal scanning laser microscopy in exfoliation syndrome, exfoliation syndrome suspect and normal eyes. Acta Ophthalmol. 2011;89(3):241–7. PubMed:19860778.CrossRefPubMed
15.
Winchester K, Mathers WD, Sutphin JE. Diagnosis of Aspergillus keratitis in vivo with confocal microscopy. Cornea. 1997;16(1):27–31. PubMed: 8985630.CrossRefPubMed
16.
Lim LL, Hoang L, Wong T, et al. Intravital microscopy of leukocyte-endothelial dynamics using the Heidelberg confocal laser microscope in scleritis and allergic conjunctivitis. Mol Vis. 2006;12(10):1302–5. PubMed: 17110913.PubMed
17.
Wang N, Lai M, Zhou W. Quantitative real time measurement of iris configuration in human eyes. Yan Ke Xue Bao. 1997;13(1):29–34. PubMed: 11189323.PubMed
18.
Garibaldi DC, Schein OD, Jun A. Features of the iridocorneal endothelial syndrome on confocal microscopy. Cornea. 2005;24(3):349–51. PubMed: 15778613.CrossRefPubMed
19.
Amos WB, White JG. How the Confocal Laser Scanning Microscope entered Biological Research. Biol Cell. 2003;95(6):335–42. PubMed: 14519550.CrossRefPubMed
20.
Grupcheva CN, McGhee CN, Dean S, Craig JP. In vivo confocal microscopic characteristics of iridocorneal endothelial syndrome. Clin Experiment Ophthalmol. 2004;32(3):275–83. PubMed: 15180840.CrossRefPubMed
21.
Alvarado JA, Murphy CG, Juster RP, et al. Pathogenesis of Chandler’s syndrome, essential iris atrophy and the Cogan-Reese syndrome. I. Alterations of the corneal endothelium. Invest Ophthalmol Vis Sci. 1986;27(6):853–72. PubMed: 3710729.PubMed
22.
Góes RM, Barbosa FL, de Faria-E-Sousa SJ, et al. Proliferation of the vascular endothelium of the iris following total debridement of the corneal epithelium and limbal excision of rabbits. Graefes Arch Clin Exp Ophthalmol. 2008;246(7):999–1007. PubMed: 18431590.CrossRefPubMed
Metadata
Title
Iris ultrastructure in patients with synechiae as revealed by in vivo laser scanning confocal microscopy
In vivo iris ultrastructure in patients with Synechiae by Laser Scanning Confocal Microscopy
Authors
Ming Li
Hongbo Cheng
Ping Guo
Chun Zhang
Song Tang
Shusheng Wang
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Ophthalmology / Issue 1/2016
Electronic ISSN: 1471-2415
DOI
https://doi.org/10.1186/s12886-016-0224-2

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