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BMC Cancer
Published in: BMC Cancer 1/2018

Open Access 01-12-2018 | Research article

RETRACTED ARTICLE: p300 promotes proliferation, migration, and invasion via inducing epithelial-mesenchymal transition in non-small cell lung cancer cells

Authors: Xue Hou, Run Gong, Jianhua Zhan, Ting Zhou, Yuxiang Ma, Yuanyuan Zhao, Yaxiong Zhang, Gang Chen, Zhonghan Zhang, Shuxiang Ma, Xi Chen, Fangfang Gao, Shaodong Hong, Fan Luo, Wenfeng Fang, Yunpeng Yang, Yan Huang, Likun Chen, Haoxian Yang, Li Zhang

Published in: BMC Cancer | Issue 1/2018

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Abstract

Background

Histone acetyltransferase p300 is a crucial transcriptional coactivator and has been implicated as a poor prognostic factor in human cancers. However, little is known about the substantial functions and mechanisms of p300 in NSCLC proliferation and distant metastasis.

Methods

We constructed p300 down-regulated and up-regulated cell lines through RNAi and recombinant plasmid transfection. Cell Counting Kit-8 assays were used to test the cell proliferation and confirmed by colony formation assays. Wound healing assays and transwell chamber assays were used to test the migration and invasion ability. Based upon these results, we measured the epithelial markers and mesenchymal markers after regulating p300 expression to explore epithelial-mesenchymal transition as a potential mechanism of p300 promoting NSCLC metastasis.

Results

In NSCLC cells NCI-H1975 and NCI-H1993, down-regulation of p300 leads to inhibition of cell proliferation and colony formation. Cells with reduced p300 expression also demonstrate inhibited migration and invasion ability. Contrarily, up-regulation of p300 significantly enhanced the proliferation, colony formation, migration and invasion ability of NCI-H460. Importantly, further investigation shows that decreased p300 expression is associated with reduced expression of mesenchymal markers and increased expression of epithelial markers, while up-regulated p300 expression correlated with decreased expression of epithelial markers and increased expression of mesenchymal markers.

Conclusions

As a crucial tumor promoter, p300 promotes cell proliferation, migration, and invasion in NSCLC cells. Epithelial-mesenchymal transition is a potential mechanism of p300 promoting NSCLC metastasis.
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Metadata
Title
RETRACTED ARTICLE: p300 promotes proliferation, migration, and invasion via inducing epithelial-mesenchymal transition in non-small cell lung cancer cells
Authors
Xue Hou
Run Gong
Jianhua Zhan
Ting Zhou
Yuxiang Ma
Yuanyuan Zhao
Yaxiong Zhang
Gang Chen
Zhonghan Zhang
Shuxiang Ma
Xi Chen
Fangfang Gao
Shaodong Hong
Fan Luo
Wenfeng Fang
Yunpeng Yang
Yan Huang
Likun Chen
Haoxian Yang
Li Zhang
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-018-4559-3

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