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BMC Cancer
Published in: BMC Cancer 1/2018

Open Access 01-12-2018 | Research article

GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma

Authors: Xing Wei, Kun Zhang, Haifeng Qin, Jinlong Zhu, Qiaoxi Qin, Yang Yu, Hong Wang

Published in: BMC Cancer | Issue 1/2018

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Abstract

Background

Lung adenocarcinoma is the most common type of lung cancer and one of the most lethal and prevalent cancers. Aberrant glycosylation was common and essential in tumorigenesis, with fucosylation as one of the most common types disrupted in cancers. However, it is still unknown whether genes involved in fucosylation are important for lung adenocarcinoma development and process.

Methods

GMDS is involved in cellular fucosylation. Here we examined GMDS expression level at both mRNA and protein level in lung adenocarcinoma. The impact of GMDS knockdown on lung adenocarcinoma in vitro and in vivo was investigated. Transcriptome changes with GMDS knockdown in lung adenocarcinoma cells were also examined to provide insights into related molecular mechanisms.

Results

GMDS expression is significantly upregulated in lung adenocarcinoma at both mRNA and protein levels. Lentivirus-mediated shRNA strategy inhibited GMDS expression efficiently in human lung adenocarcinoma cells A549 and H1299, and GMDS knockdown impaired cell proliferation, colony formation ability, induced cell cycle arrest, and apoptosis in both cell lines. Furthermore, GMDS knockdown inhibited tumorigenesis in a xenograft mice model of lung adenocarcinoma. Microarray analysis explored the GMDS-mediated molecular network and revealed that the CASP8-CDKN1A axis might be critical for lung adenocarcinoma development.

Conclusions

These findings suggest that GMDS upregulation is critical for cell proliferation and survival in human lung adenocarcinoma and might serve as a potential biomarker for lung adenocarcinoma diagnosis and treatment.
Appendix
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Metadata
Title
GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma
Authors
Xing Wei
Kun Zhang
Haifeng Qin
Jinlong Zhu
Qiaoxi Qin
Yang Yu
Hong Wang
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-018-4524-1

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