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BMC Cancer
Published in: BMC Cancer 1/2018

Open Access 01-12-2018 | Research article

Microsatellite instability testing in colorectal cancer using the QiaXcel advanced platform

Authors: Isabel Förster, Michael Brockmann, Oliver Schildgen, Verena Schildgen

Published in: BMC Cancer | Issue 1/2018

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Abstract

Background

Microsatellite instability (MSI) is a major predictive and diagnostic marker in several cancers including colorectal carcinomas. Diagnostic testing for microsatellites is generally performed using capillary sequencers, which requires expensive high-end equipment including expensive chemistry using fluorescent dyes labelling the PCR products of interest. In this study we have modified such a diagnostic protocol and established the microsatellite testing on the QiaXcel Advanced platform.

Methods

MSI testing was based on a previously established protocol describing a multiplex PCR followed by fluorescent detection of PCR products in a capillary sequencing device. Ten microsatellites were included in the new protocol: BAT25, BAT26, BAT40, D2s123, D10s197, D13s153, D17s250, D18s58, D5s346, and MycI. In this protocol the PCR was demultiplexed and established on the QiaXcel Advanced system (Qiagen, Hilden, Germany).

Results

Making use of a series of FFPE control samples with known MSI status including those with and without MSI a protocol for MSI testing was successfully established on the QiaXcel Advanced platform.

Conclusions

MSI testing for human colorectal cancers using the QiaXcel Advanced system could serve as an economic acceptable tool for rapid diagnostics in laboratories that do not have access to a capillary sequencing unit.
Literature
1.
Dean DA, Wadl PA, Hadziabdic D, Wang X, Trigiano RN. Analyzing microsatellites using the QIAxcel system. Methods Mol Biol. 2013;1006:223–43.CrossRefPubMed
2.
Manrique P, Hoshi M, Fasabi M, Nolasco O, Yori P, Calderon M, Gilman RH, Kosek MN, Vinetz JM, Gamboa D. Assessment of an automated capillary system for plasmodium vivax microsatellite genotyping. Malar J. 2015;14:326.CrossRefPubMedPubMedCentral
3.
Coetzer WG, Downs CT, Perrin MR, Willows-Munro S. Testing of microsatellite multiplexes for individual identification of cape parrots (Poicephalus robustus): paternity testing and monitoring trade. PeerJ. 2017;5:e2900.CrossRefPubMedPubMedCentral
4.
Hoekert WE, Neufeglise H, Schouten AD, Menken SB. Multiple paternity and female-biased mutation at a microsatellite locus in the olive ridley sea turtle (Lepidochelys olivacea). Heredity. 2002;89(2):107–13.CrossRefPubMed
5.
Ingvarsson S, Finnsdottir V, Sigurdsson A, Geirsson G. Population studies and validation of paternity determinations by six microsatellite loci. J Forensic Sci. 2000;45(3):692–5.CrossRefPubMed
6.
McVay JD, Rodriguez D, Rainwater TR, Dever JA, Platt SG, McMurry ST, Forstner MR, Densmore LD 3rd. Evidence of multiple paternity in Morelet's crocodile (Crocodylus moreletii) in Belize, CA, inferred from microsatellite markers. J Exp Zool A Ecol Genet Physiol. 2008;309((10):643–8.CrossRef
7.
Sakaoka K, Suzuki I, Kasugai N, Fukumoto Y. Paternity testing using microsatellite DNA markers in captive Adelie penguins (Pygoscelis adeliae). Zoo biology. 2014;33(5):463–70.PubMed
8.
Song SD, Drew RA, Hughes JM. Multiple paternity in a natural population of a wild tobacco fly, Bactrocera cacuminata (Diptera: Tephritidae), assessed by microsatellite DNA markers. Mol Ecol. 2007;16(11):2353–61.CrossRefPubMed
9.
Xue D, Zhang T, Liu JX. Microsatellite evidence for high frequency of multiple paternity in the marine gastropod Rapana venosa. PLoS One. 2014;9(1):e86508.CrossRefPubMedPubMedCentral
10.
Bettstetter M, Dechant S, Ruemmele P, Grabowski M, Keller G, Holinski-Feder E, Hartmann A, Hofstaedter F, Dietmaier W. Distinction of hereditary nonpolyposis colorectal cancer and sporadic microsatellite-unstable colorectal cancer through quantification of MLH1 methylation by real-time PCR. Clinical cancer research : an official journal of the American Association for Cancer Research. 2007;13(11):3221–8.CrossRef
11.
Bettstetter M, Woenckhaus M, Wild PJ, Rummele P, Blaszyk H, Hartmann A, Hofstadter F, Dietmaier W. Elevated nuclear maspin expression is associated with microsatellite instability and high tumour grade in colorectal cancer. J Pathol. 2005;205(5):606–14.CrossRefPubMed
12.
Dietmaier W, Gansbauer S, Beyser K, Renke B, Hartmann A, Rummele P, Jauch KW, Hofstadter F, Ruschoff J. Microsatellite instability in tumor and nonneoplastic colorectal cells from hereditary non-polyposis colorectal cancer and sporadic high microsatellite-instable tumor patients. Pathobiology : journal of immunopathology, molecular and cellular biology. 2000;68(4–5):227–31.CrossRef
13.
Giuffre G, Muller A, Brodegger T, Bocker-Edmonston T, Gebert J, Kloor M, Dietmaier W, Kullmann F, Buttner R, Tuccari G, et al. Microsatellite analysis of hereditary nonpolyposis colorectal cancer-associated colorectal adenomas by laser-assisted microdissection: correlation with mismatch repair protein expression provides new insights in early steps of tumorigenesis. The Journal of molecular diagnostics : JMD. 2005;7(2):160–70.CrossRefPubMedPubMedCentral
14.
Wild PJ, Reichle A, Andreesen R, Rockelein G, Dietmaier W, Ruschoff J, Blaszyk H, Hofstadter F, Hartmann A. Microsatellite instability predicts poor short-term survival in patients with advanced breast cancer after high-dose chemotherapy and autologous stem-cell transplantation. Clinical cancer research : an official journal of the American Association for Cancer Research. 2004;10(2):556–64.CrossRef
15.
Popat S, Hubner R, Houlston RS. Systematic review of microsatellite instability and colorectal cancer prognosis. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 2005;23(3):609–18.CrossRef
16.
Dietmaier W. Microsatellite instability. A new predictive marker (?). Pathologe. 2010;31(Suppl 2):268–73.CrossRefPubMed
17.
Hause RJ, Pritchard CC, Shendure J, Salipante SJ. Classification and characterization of microsatellite instability across 18 cancer types. Nat Med. 2016;22(11):1342–50.CrossRefPubMed
18.
Pecorino B, Rubino C, Guardala VF, Galia A, Scollo P. Genetic screening in young women diagnosed with endometrial cancer. J Gynecol Oncol. 2017;28(1):e4.CrossRefPubMed
19.
Boland CR, Thibodeau SN, Hamilton SR, Sidransky D, Eshleman JR, Burt RW, Meltzer SJ, Rodriguez-Bigas MA, Fodde R, Ranzani GN, et al. A National Cancer Institute workshop on microsatellite instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res. 1998;58(22):5248–57.PubMed
20.
Dietmaier W, Wallinger S, Bocker T, Kullmann F, Fishel R, Ruschoff J. Diagnostic microsatellite instability: definition and correlation with mismatch repair protein expression. Cancer Res. 1997;57(21):4749–56.PubMed
21.
Schiemann U, Muller-Koch Y, Gross M, Daum J, Lohse P, Baretton G, Muders M, Mussack T, Kopp R, Holinski-Feder E. Extended microsatellite analysis in microsatellite stable, MSH2 and MLH1 mutation-negative HNPCC patients: genetic reclassification and correlation with clinical features. Digestion. 2004;69(3):166–76.CrossRefPubMed
22.
Dietmaier W, Hofstadter F. Detection of microsatellite instability by real time PCR and hybridization probe melting point analysis. Laboratory investigation; a journal of technical methods and pathology. 2001;81(10):1453–6.CrossRefPubMed
Metadata
Title
Microsatellite instability testing in colorectal cancer using the QiaXcel advanced platform
Authors
Isabel Förster
Michael Brockmann
Oliver Schildgen
Verena Schildgen
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-018-4400-z

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