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BMC Cancer
Published in: BMC Cancer 1/2018

Open Access 01-12-2018 | Research article

Overexpression of endothelin B receptor in glioblastoma: a prognostic marker and therapeutic target?

Authors: Suhas Vasaikar, Giorgos Tsipras, Natalia Landázuri, Helena Costa, Vanessa Wilhelmi, Patrick Scicluna, Huanhuan L. Cui, Abdul-Aleem Mohammad, Belghis Davoudi, Mingmei Shang, Sharan Ananthaseshan, Klas Strååt, Giuseppe Stragliotto, Afsar Rahbar, Kum Thong Wong, Jesper Tegner, Koon-Chu Yaiw, Cecilia Söderberg-Naucler

Published in: BMC Cancer | Issue 1/2018

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Abstract

Background

Glioblastoma (GBM) is the most common malignant brain tumor with median survival of 12-15 months. Owing to uncertainty in clinical outcome, additional prognostic marker(s) apart from existing markers are needed. Since overexpression of endothelin B receptor (ETBR) has been demonstrated in gliomas, we aimed to test whether ETBR is a useful prognostic marker in GBM and examine if the clinically available endothelin receptor antagonists (ERA) could be useful in the disease treatment.

Methods

Data from The Cancer Genome Atlas and the Gene Expression Omnibus database were analyzed to assess ETBR expression. For survival analysis, glioblastoma samples from 25 Swedish patients were immunostained for ETBR, and the findings were correlated with clinical history. The druggability of ETBR was assessed by protein-protein interaction network analysis. ERAs were analyzed for toxicity in in vitro assays with GBM and breast cancer cells.

Results

By bioinformatics analysis, ETBR was found to be upregulated in glioblastoma patients, and its expression levels were correlated with reduced survival. ETBR interacts with key proteins involved in cancer pathogenesis, suggesting it as a druggable target. In vitro viability assays showed that ERAs may hold promise to treat glioblastoma and breast cancer.

Conclusions

ETBR is overexpressed in glioblastoma and other cancers and may be a prognostic marker in glioblastoma. ERAs may be useful for treating cancer patients.
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Metadata
Title
Overexpression of endothelin B receptor in glioblastoma: a prognostic marker and therapeutic target?
Authors
Suhas Vasaikar
Giorgos Tsipras
Natalia Landázuri
Helena Costa
Vanessa Wilhelmi
Patrick Scicluna
Huanhuan L. Cui
Abdul-Aleem Mohammad
Belghis Davoudi
Mingmei Shang
Sharan Ananthaseshan
Klas Strååt
Giuseppe Stragliotto
Afsar Rahbar
Kum Thong Wong
Jesper Tegner
Koon-Chu Yaiw
Cecilia Söderberg-Naucler
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2018
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-018-4012-7

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