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BMC Cancer
Published in: BMC Cancer 1/2017

Open Access 01-12-2017 | Research article

Synergistic inhibition of tumor growth by combination treatment with drugs against different subpopulations of glioblastoma cells

Authors: Chia-Hsin Chang, Wei-Ting Liu, Hui-Chi Hung, Chia-Yu Gean, Hong-Ming Tsai, Chun-Lin Su, Po-Wu Gean

Published in: BMC Cancer | Issue 1/2017

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Abstract

Background

Glioma stem cells (GSCs) contribute to tumor recurrence and drug resistance. This study characterizes the tumorigenesis of CD133+ cells and their sensitivity to pharmacological inhibition.

Methods

GSCs from human U87 and rat C6 glioblastoma cell lines were isolated via magnetic cell sorting using CD133 as a cancer stem cell marker. Cell proliferation was determined using the WST-1 assay. An intracranial mouse model and bioluminescence imaging were used to assess the effects of drugs on tumor growth in vivo.

Results

CD133+ cells expressed stem cell markers and exhibited self-renewal and enhanced tumor formation. Minocycline (Mino) was more effective in reducing the survival rate of CD133+ cells, whereas CD133− cells were more sensitive to inhibition by the signal transducer and activator of transcription 3 (STAT3) inhibitor. Inhibition of STAT3 decreased the expression of CD133+ stem cell markers. The combination of Mino and STAT3 inhibitor synergistically reduced the cell viability of glioma cells. Furthermore, this combination synergistically suppressed tumor growth in nude mice.

Conclusion

The results suggest that concurrent targeting of different subpopulations of glioblastoma cells may be an effective therapeutic strategy for patients with malignant glioma.
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Metadata
Title
Synergistic inhibition of tumor growth by combination treatment with drugs against different subpopulations of glioblastoma cells
Authors
Chia-Hsin Chang
Wei-Ting Liu
Hui-Chi Hung
Chia-Yu Gean
Hong-Ming Tsai
Chun-Lin Su
Po-Wu Gean
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2017
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-017-3924-y

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