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BMC Cancer
Published in: BMC Cancer 1/2017

Open Access 01-12-2017 | Research article

miR-663a regulates growth of colon cancer cells, after administration of antimicrobial peptides, by targeting CXCR4-p21 pathway

Authors: Kengo Kuroda, Tomokazu Fukuda, Marija Krstic-Demonacos, Constantinos Demonacos, Kazuhiko Okumura, Hiroshi Isogai, Miwa Hayashi, Kazuki Saito, Emiko Isogai

Published in: BMC Cancer | Issue 1/2017

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Abstract

Background

Antimicrobial peptides (AMPs) play important roles in the innate immune system of all life forms and recently have been characterized as multifunctional peptides that have a variety of biological roles such as anticancer agents. However, detailed mechanism of antimicrobial peptides on cancer cells is still largely unknown.

Methods

miRNA array and real-time qPCR were performed to reveal the behavior of miRNA in colon cancer HCT116 cells during the growth suppression induced by the AMPs. Establishment of miR-663a over-expressing HCT116 cells was carried out for the evaluation of growth both in vitro and in vivo. To identify the molecular mechanisms, we used western blotting analysis.

Results

miR-663a is upregulated by administration of the human cathelicidin AMP, LL-37, and its analogue peptide, FF/CAP18, in the colon cancer cell line HCT116. Over-expression of miR-663a caused anti-proliferative effects both in vitro and in vivo. We also provide evidence supporting the view that these effects are attributed to suppression of the expression of the chemokine receptor CXCR4, resulting in the abrogation of phosphorylation of Akt and cell cycle arrest in G2/M via p21 activation.

Conclusions

This study contributes to the understanding of the AMPs’ mediated anti-cancer mechanisms in colon cancer cells and highlights the possibility of using AMPs and miRNAs towards developing future strategies for cancer therapy.
Appendix
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Metadata
Title
miR-663a regulates growth of colon cancer cells, after administration of antimicrobial peptides, by targeting CXCR4-p21 pathway
Authors
Kengo Kuroda
Tomokazu Fukuda
Marija Krstic-Demonacos
Constantinos Demonacos
Kazuhiko Okumura
Hiroshi Isogai
Miwa Hayashi
Kazuki Saito
Emiko Isogai
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2017
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-016-3003-9

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