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BMC Cancer
Published in: BMC Cancer 1/2016

Open Access 01-12-2016 | Research article

MPO density in primary cancer biopsies of ovarian carcinoma enhances the indicative value of IL-17 for chemosensitivity

Authors: Raoul A. Droeser, Robert Mechera, Silvio Däster, Benjamin Weixler, Marko Kraljević, Tarik Delko, Uwe Güth, Sylvia Stadlmann, Luigi Terracciano, Gad Singer

Published in: BMC Cancer | Issue 1/2016

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Abstract

Background

Cancer of the ovary is mostly discovered at a late stage and cannot be removed by surgery alone. Therefore surgery is usually followed by adjuvant chemotherapy. However, few reliable biomarkers exist to predict response to chemotherapy of ovarian cancer. Previously, we could demonstrate that IL-17 density is indicative for chemosensitivity. This study focuses on the predictive value of myeloperoxidase (MPO) concerning response to chemotherapy of ovarian cancer.

Methods

Biopsies of mostly high-grade primary serous ovarian carcinomas and their matched recurrences were stained with MPO after fixation in formalin and embedding in paraffin. For this staining the technique of tissue-microarray was used. Recurrence within 6 months of the completion of platinum-based chemotherapy was defined as chemoresistance as previously publised. Data for MPO could be analyzed in 92 biopsies.

Results

MPO and IL-17 positive immune cells correlated significantly in biopsies of primary and recurrent carcinomas (rs = 0.41; p = 0.004 and rs = 0.40; p = 0.007, respectively). MPO expression alone did not predict response to chemotherapy, but in multivariate cox regression analysis including age, residual disease, number of chemotherapy cycles, FIGO classification and combined categorized MPO and IL-17 cell densities of primary cancer biopsies, the combination of both immune markers was an independent prognostic factor for recurrence-free survival (p = 0.013, HR = .23, 95CI = 0.07–0.73). There was no chemoresistant patient in the subgroup of MPO + IL-17+, neither in primary nor in recurrent cancer biopsies.

Conclusions

High MPO positive cell density enhances the indicative value of IL-17 for response to chemotherapy in ovarian carcinoma. Although, these results have to be validated in a larger cohort.
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Metadata
Title
MPO density in primary cancer biopsies of ovarian carcinoma enhances the indicative value of IL-17 for chemosensitivity
Authors
Raoul A. Droeser
Robert Mechera
Silvio Däster
Benjamin Weixler
Marko Kraljević
Tarik Delko
Uwe Güth
Sylvia Stadlmann
Luigi Terracciano
Gad Singer
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2016
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-016-2673-7

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