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BMC Cancer
Published in: BMC Cancer 1/2015

Open Access 01-12-2015 | Research article

MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism

Authors: Bo Wang, Zhan-long Shen, Ke-wei Jiang, Gang Zhao, Chun-you Wang, Yi-chao Yan, Yang Yang, Ji-zhun Zhang, Chao Shen, Zhi-dong Gao, Ying-jiang Ye, Shan Wang

Published in: BMC Cancer | Issue 1/2015

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Abstract

Background

Recent studies have indicated the possible function of miR-217 in tumorigenesis. However, the roles of miR-217 in colorectal cancer (CRC) are still largely unknown.

Methods

We examined the expression of miR-217 and AEG-1 in 50 CRC tissues and the corresponding noncancerous tissues by qRT-PCR. The clinical significance of miR-217 was analyzed. CRC cell lines with miR-217 upregulation and AEG-1 silencing were established and the effects on tumor growth in vitro and in vivo were assessed. Dual-luciferase reporter gene assays were also performed to investigate the interaction between miR-217 and AEG-1.

Results

Our data demonstrated that miR-217 was significantly downregulated in 50 pairs of colorectal cancer tissues. MiR-217 expression levels were closely correlated with tumor differentiation. Moreover, decreased miR-217 expression was also associated with shorter overall survival of CRC patients. MiR-217 overexpression significantly inhibited proliferation, colony formation and invasiveness of CRC cells by promoting apoptosis and G0/G1 phase arrest. Interestingly, ectopic miR-217 expression decreased AEG-1 expression and repressed luciferase reporter activity associated with the AEG-1 3′-untranslated region (UTR). AEG-1 silencing resulted in similar biological behavior changes to those associated with miR-217 overexpression. Finally, in a nude mouse xenografted tumor model, miR-217 overexpression significantly suppressed CRC cell growth.

Conclusions

Our findings suggest that miR-217 has considerable value as a prognostic marker and potential therapeutic target in CRC.
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Metadata
Title
MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism
Authors
Bo Wang
Zhan-long Shen
Ke-wei Jiang
Gang Zhao
Chun-you Wang
Yi-chao Yan
Yang Yang
Ji-zhun Zhang
Chao Shen
Zhi-dong Gao
Ying-jiang Ye
Shan Wang
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2015
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-015-1438-z

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