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BMC Cancer
Published in: BMC Cancer 1/2015

Open Access 01-12-2015 | Research article

ACTN4 and the pathways associated with cell motility and adhesion contribute to the process of lung cancer metastasis to the brain

Authors: Yufei Gao, Guanghu Li, Liankun Sun, Yichun He, Xiaoyan Li, Zhi Sun, Jihan Wang, Yang Jiang, Jingwei Shi

Published in: BMC Cancer | Issue 1/2015

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Abstract

Background

The aim of this study was to identify critical gene pathways that are associated with lung cancer metastasis to the brain.

Methods

The RNA-Seq approach was used to establish the expression profiles of a primary lung cancer, adjacent benign tissue, and metastatic brain tumor from a single patient. The expression profiles of these three types of tissues were compared to define differentially expressed genes, followed by serial-cluster analysis, gene ontology analysis, pathway analysis, and knowledge-driven network analysis. Reverse transcription–polymerase chain reaction (RT-PCR) was used to validate the expression of essential candidate genes in tissues from ten additional patients.

Results

Differential gene expression among these three types of tissues was classified into multiple clusters according to the patterns of their alterations. Further bioinformatic analysis of these expression profile data showed that the network of the signal transduction pathways related to actin cytoskeleton reorganization, cell migration, and adhesion was associated with lung cancer metastasis to the brain. The expression of ACTN4 (actinin, alpha 4), a cytoskeleton protein gene essential for cytoskeleton organization and cell motility, was significantly elevated in the metastatic brain tumor but not in the primary lung cancer tissue.

Conclusions

The signaling pathways involved in the regulation of cytoskeleton reorganization, cell motility, and focal adhesion play a role in the process of lung cancer metastasis to the brain. The contribution of ACTN4 to the process of lung cancer metastasis to the brain could be mainly through regulation of actin cytoskeleton reorganization, cell motility, and focal adhesion.
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Metadata
Title
ACTN4 and the pathways associated with cell motility and adhesion contribute to the process of lung cancer metastasis to the brain
Authors
Yufei Gao
Guanghu Li
Liankun Sun
Yichun He
Xiaoyan Li
Zhi Sun
Jihan Wang
Yang Jiang
Jingwei Shi
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2015
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-015-1295-9

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