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BMC Cancer
Published in: BMC Cancer 1/2015

Open Access 01-12-2015 | Research article

Prognostic value of carbonic anhydrase VII expression in colorectal carcinoma

Authors: Guang-Zhen Yang, Liang Hu, Jian Cai, Hai-Yang Chen, Yu Zhang, Dan Feng, Chen-Ye Qi, Yan-Xia Zhai, Hui Gong, Hao Fu, Qing-Ping Cai, Chun-Fang Gao

Published in: BMC Cancer | Issue 1/2015

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Abstract

Background

Carbonic anhydrases (CAs) have been implicated in the pathogenesis of human cancers. Carbonic anhydrase VII (CA7), a member of the CA gene family, was recently demonstrated to be expressed in several human tissues including colon. Nevertheless, the expression and clinical relevance of CA7 in colorectal carcinoma (CRC) has not been investigated.

Methods

Real-time PCR, western blot, and immunohistochemistry analyses were used to determine CA7 expression in CRC clinical samples. The correlation of CA7 expression with clinicopathologic features was assessed in 228 patients from Luoyang, China (training cohort) and validated in 151 patients from Shanghai, China (validation cohort). Kaplan-Meier and Cox proportional regression analyses were used to estimate the association between CA7 expression and patients’ survival.

Results

CA7 expression was frequently downregulated in CRC tissues at both the mRNA and protein levels. Reduced expression of CA7 was significantly correlated with poor differentiation, positive lymph node metastasis, advanced TNM stage and unfavorable clinical outcome not only in the training cohort but also in the validation set. Survival analysis indicated that patients with lower CA7 expression had a significantly shorter disease-specific survival (DSS) than those with higher CA7 expression. Importantly, further stage-based analyses revealed that decreased CA7 expression significantly predicted poor DSS and was an independent adverse prognostic indicator for patients with early stage tumors in both cohorts.

Conclusions

Our results indicate that decreased expression of CA7 correlates with disease progression and predicts poor prognosis in CRC, especially for patients with early stage tumors.
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Metadata
Title
Prognostic value of carbonic anhydrase VII expression in colorectal carcinoma
Authors
Guang-Zhen Yang
Liang Hu
Jian Cai
Hai-Yang Chen
Yu Zhang
Dan Feng
Chen-Ye Qi
Yan-Xia Zhai
Hui Gong
Hao Fu
Qing-Ping Cai
Chun-Fang Gao
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2015
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-015-1216-y

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