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BMC Pregnancy and Childbirth
Published in: BMC Pregnancy and Childbirth 1/2016

Open Access 01-12-2016 | Research article

The effects of embryo culture media on the birthweight of singletons via fresh or frozen-thawed embryo transfer: a large-scale retrospective study

Authors: Fang Gu, Mingfen Deng, Jun Gao, Zilian Wang, Chenhui Ding, Yanwen Xu, Canquan Zhou

Published in: BMC Pregnancy and Childbirth | Issue 1/2016

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Abstract

Background

Embryo culture media used for IVF treatment might affect fetal growth and thus birthweight of the newborns.

Methods

A retrospective study was conducted in South China using data from 2370 singleton neonates born after IVF/ICSI between 2009 and 2012. Two culture media, i.e., either Vitrolife or SAGE were used as embryo culture media during the study period. Neonates’ birthweights were compared between the two embryo culture media groups.

Results

Among the 2370 singletons, 1755 cases came from fresh cleavage embryo transfer while 615 were from frozen-thawed cleavage embryo transfer. Within the fresh embryo transfer newborns, no statistical difference was observed in either birthweight (mean ± SD: 3196.0 ± 468.9 versus 3168.4 ± 462.0g, p > 0.05) or adjusted birthweight controlled for gestational age and gender (z-score mean ± SD: 0.11 ± 1.02 versus 0.11 ± 0.99 g, P > 0.05) between the Vitrolife (n = 419) and the SAGE group (n = 1336). Likewise within frozen embryo transfer neotates, no statistical difference of the birthweight (3300.6 ± 441.3 vs.3256.0 ± 466.7 g, P > 0.05) and adjusted birthweight (0.30 ± 0.99 g versus 0.29 ± 0.97 g, P > 0.05) was found between the Vitrolife (n = 202) and the SAGE group (n = 413). The sex ratio [OR1.17, 95 % CI (0.94–1.46)/OR1.1, 95 % CI (0.78–1.54)], rate of small for gestational age [OR1.14, 95 % CI (0.82–1.59)/OR1.06, 95 % CI (0.56–2.02)] and large for gestational age [OR1.07, 95 % CI (0.64–1.76)/OR0.98, 95 % CI (0.47–2.02)] in fresh and frozen-thawed subgourps are all comparable respectively between the two culture media. No group differences were found in the rate of low birthweight and macosomia. Multiple linear regression analysis demonstrated that maternal weight, gestational age, frozen-thawed embryo transfer and infant gender were significantly related to neonatal birthweight (P < 0.001).

Conclusions

It appears that embryos cultured in SAGE or Vitrolife media after fresh or frozen-thawed cleavage embryo transfer did not affect neonate’s birthweight.
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Metadata
Title
The effects of embryo culture media on the birthweight of singletons via fresh or frozen-thawed embryo transfer: a large-scale retrospective study
Authors
Fang Gu
Mingfen Deng
Jun Gao
Zilian Wang
Chenhui Ding
Yanwen Xu
Canquan Zhou
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Pregnancy and Childbirth / Issue 1/2016
Electronic ISSN: 1471-2393
DOI
https://doi.org/10.1186/s12884-016-1077-7

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