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BMC Anesthesiology
Published in: BMC Anesthesiology 1/2015

Open Access 01-12-2015 | Research article

Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents

Authors: Huan Ma, Daozheng Huang, Minzhou Zhang, Xin Huang, Shiyu Ma, Shuai Mao, Wenhui Li, Yanfen Chen, Liheng Guo

Published in: BMC Anesthesiology | Issue 1/2015

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Abstract

Backgroud

Lung ultrasound (LUS) can diagnose extravacular lung water (EVLW) through the visualization of B lines in both humans and large animals. However, there are no published data on the use of ultrasound to detect EVLW in rats, the gold standard to evaluate of EVLW in rats is post-mortem gravimetric analysis. The present study was designed to determine the similarity between lung sonography and gravimetric measurements of EVLW in rats in an acute lung injury (ALI) model.

Methods

Thirty male Sprague–Dawley rats were randomized into control and experimental groups. The B lines were measured byLUS at baseline. ALI was induced by the intravenous administration of oleic acid (OA) at a dose of 9 ul/100 mg, and controls were injected the same amount of isotonic saline. After 1 h, B-lines were measured by LUS in each rat following the induction of ALI. At the end of each experiment, both lungs were dissected, weighed and dried to determine wet/dry weight ratio according to the standard gravimetric methodology. Lung samples from three rats in each group were examined histologically.

Results

B-lines were present in all rats from experimental group at 1 h point after OA injection. The statistical correlation between the two methods of assessing EVLW provided an r = 0.834 (p < 0.001). Repeatability studies of the LUS technique (Bland-Altman plots) showed good intra-observer and inter-observer reproducibility.

Conclusion

The data suggest that, in an experimental rat model of ALI, B lines score as assessed by LUS can provide an easy, semi-quantitative, noninvasive.
Real-time index of EVLW which is strongly correlated to experimental gravimetric assessments.
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Metadata
Title
Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents
Authors
Huan Ma
Daozheng Huang
Minzhou Zhang
Xin Huang
Shiyu Ma
Shuai Mao
Wenhui Li
Yanfen Chen
Liheng Guo
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Anesthesiology / Issue 1/2015
Electronic ISSN: 1471-2253
DOI
https://doi.org/10.1186/s12871-015-0146-1

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