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Journal of Ophthalmic Inflammation and Infection
Published in: Journal of Ophthalmic Inflammation and Infection 1/2018

Open Access 01-12-2018 | Original research

Outcome of polymerase chain reaction (PCR) analysis in 100 suspected cases of infectious uveitis

Published in: Journal of Ophthalmic Inflammation and Infection | Issue 1/2018

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Abstract

Background

Polymerase chain reaction (PCR) analysis is an important tool in the diagnosis of infectious uveitis. A retrospective, interventional study of PCR analysis of ocular fluid in suspected infectious uveitis cases between January 2014 to July 2016 was done. Nested, real-time and broad range PCR was performed for detection of the genome of Mycobacterium tuberculosis, herpes virus family, Chikungunya virus, Toxoplasma gondii, fungus, eubacterium and propionibacterium acne.

Results

Total of 100 cases included, mean age was 39.2 ± 15.4 years. Uveitis was unilateral in 82% and granulomatous in 40%. Mean visual acuity at the initial visit and final visit was 0.73 logMar and 0.63 logMar respectively. PCR analysis confirmed the clinical diagnosis in 70.1% patients. The sensitivity, specificity, positive predictive value and negative predictive value of PCR analysis was 90.2%, 93.9%, 93.9% and 90.2% respectively. The quantitative value of real-time M. tb. Positive PCR ranged from 32c/ml to 2722 c/ml.

Conclusions

PCR assay is an accurate technique with high sensitivity and specificity to diagnose the DNA genome in infectious uveitis.
Literature
1.
Sabhapandit S, Murthy SI, Singh VM, Gaitonde K, Gopal M, Marsonia K, Sajid S, Babu K. Epidemiology and clinical features of uveitis from urban populations in South India. Ocul Immunol Inflamm. 2017;25(sup1):S39-45.
2.
Verhagen C, Bruinenberg M, Rothova A, Baarsma G, Der LA, Ooyman F et al (1996) Serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis. Am J Ophthalmol 121(6):650–658CrossRefPubMed
3.
Abe T, Tsuchida K, Tamai M (1996) A comparative study of the polymerase chain reaction and local antibody production in acute retinal necrosis syndrome and cytomegalovirus retinitis. Graefes Arch Clin Exp Ophthalmol 234(7):419–424CrossRefPubMed
4.
Ganatra JB, Chandler D, Santos C, Kuppermann B, Margolis TP (2000) Viral causes of the acute retinal necrosis syndrome. Am J Ophthalmol 129(2):166–172CrossRefPubMed
5.
Tran T, Rozenberg F, Cassoux N, Rao N, LeHoang P, Bodaghi B (2003) Polymerase chain reaction analysis of aqueous humour samples in necrotising retinitis. Br J Ophthalmol 87(1):79–83CrossRefPubMedPubMedCentral
6.
Fox GM, Crouse CA, Chuang EL, Pflugfelder SC, Cleary TJ, Nelson SJ et al (1991) Detection of herpesvirus DNA in vitreous and aqueous specimens by the polymerase chain reaction. Arch Ophthalmol 109(2):266–271CrossRefPubMed
7.
MCcann JD, Margolis TP, Wong MG, Kuppermann BD, Luckie AP, Schwartz DM et al (1995) A sensitive and specific polymerase chain reaction-based assay for the diagnosis of cytomegalovirus retinitis. Am J Ophthalmol 120(2):219–226CrossRefPubMed
8.
Santos FF, Commodaro AG, Souza AV, Pinho JR, Sitnik R, Garcia C et al (2011) Real-time PCR in infectious uveitis as an alternative diagnosis. Arq Bras Oftalmol 74(4):258–261CrossRefPubMed
9.
Goto H, Mochizuki M, Yamaki K, Kotake S, Usui M, Ohno S (2007) Epidemiological survey of intraocular inflammation in Japan. Jpn J Ophthalmol 51(1):41–44CrossRefPubMed
10.
Sugita S, Ogawa M, Shimizu N, Morio T, Ohguro N, Nakai K et al (2013) Use of a comprehensive polymerase chain reaction system for diagnosis of ocular infectious diseases. Ophthalmology 120(9):1761–1768CrossRefPubMed
11.
Harper TW, Miller D, Schiffman JC, Davis JL (2009) Polymerase chain reaction analysis of aqueous and vitreous specimens in the diagnosis of posterior segment infectious uveitis. Am J Ophthalmol 147(1):140–147 e2CrossRefPubMed
12.
Davis JL, Miller DM, Ruiz P (2005) Diagnostic testing of vitrectomy specimens. Am J Ophthalmol 140(5):822–829 e2CrossRefPubMed
13.
Van Gelder RN (2003) CME review: polymerase chain reaction diagnostics for posterior segment disease. Retina 23(4):445–452CrossRefPubMed
14.
Arora S, Gupta V, Gupta A, Bambery P, Kapoor G, Sehgal S (1999) Diagnostic efficacy of polymerase chain reaction in granulomatous uveitis. Tuber Lung Dis 79(4):229–233CrossRefPubMed
15.
Gupta V, Gupta A, Rao NA (2007) Intraocular tuberculosis—an update. Surv Ophthalmol 52(6):561–587CrossRefPubMed
16.
Gargiulo F, De Francesco MA, Nascimbeni G, Turano R, Perandin F, Gandolfo E et al (2003) Polymerase chain reaction as a rapid diagnostic tool for therapy of acute retinal necrosis syndrome. J Med Virol 69(3):397–400CrossRefPubMed
17.
Biswas J, Mayr AJ, Martin WJ, Rao NA (1993) Detection of human cytomegalovirus in ocular tissue by polymerase chain reaction and in situ DNA hybridization. Graefes Arch Clin Exp Ophthalmol 231(2):66–70CrossRefPubMed
18.
Sugita S, Shimizu N, Watanabe K, Mizukami M, Morio T, Sugamoto Y et al (2008) Use of multiplex PCR and real-time PCR to detect human herpes virus genome in ocular fluids of patients with uveitis. Br J Ophthalmol 92(7):928–932CrossRefPubMedPubMedCentral
19.
De Groot-Mijnes JD, Rothova A, Van Loon AM, Schuller M, Ten Dam-Van Loon NH, De Boer JH et al (2006) Polymerase chain reaction and Goldmann-Witmer coefficient analysis are complimentary for the diagnosis of infectious uveitis. Am J Ophthalmol 141(2):313–318CrossRefPubMed
20.
Montoya JG, Parmley S, Liesenfeld O, Jaffe GJ, Remington JS (1999) Use of the polymerase chain reaction for diagnosis of ocular toxoplasmosis. Ophthalmology 106(8):1554–1563CrossRefPubMed
21.
Sugita S, Ogawa M, Inoue S, Shimizu N, Mochizuki M (2011) Diagnosis of ocular toxoplasmosis by two polymerase chain reaction (PCR) examinations: qualitative multiplex and quantitative real-time. Jpn J Ophthalmol 55(5):495–501CrossRefPubMed
22.
Santos FF, Nascimento H, Muccioli C, Costa DF, Rizzo LV, Commodaro AG et al (2015) Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR. Arq Bras Oftalmol 78(6):356–358CrossRefPubMed
Metadata
Title
Outcome of polymerase chain reaction (PCR) analysis in 100 suspected cases of infectious uveitis
Publication date
01-12-2018
Published in
Journal of Ophthalmic Inflammation and Infection / Issue 1/2018
Electronic ISSN: 1869-5760
DOI
https://doi.org/10.1186/s12348-017-0144-1

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