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Environmental Health and Preventive Medicine
Published in: Environmental Health and Preventive Medicine 1/2017

Open Access 01-12-2017 | Research article

Isolation and identification of Acanthamoeba strains from soil and tap water in Yanji, China

Authors: Yinghua Xuan, Yanqin Shen, Yuxi Ge, Gen Yan, Shanzi Zheng

Published in: Environmental Health and Preventive Medicine | Issue 1/2017

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Abstract

Background

Members of the genus Acanthamoeba are widely distributed throughout the world, and some of them are considered pathogenic, as they are capable of causing corneal and central nervous system diseases. In this study, we isolated Acanthamoeba strains from soil and tap water in Yanji, China.

Methods

We identified four strains of Acanthamoeba (CJY/S1, CJY/S2, CJY/S3, and CJY/W1) using mitochondrial DNA restriction fragment length polymorphism (mtDNA RFLP) analysis. Nuclear 18S rDNA sequences were used for phylogenetic analysis and species identification.

Results

Genotypic characterization of the isolates showed that they belonged to genotypes T4 (CJY/S1 and CJY/S2), T5 (CJY/S3), and T16 (CJY/W1). Sequence differences between CJY/S1 and Acanthamoeba castellanii Neff, CJY/S2 and Acanthamoeba KA/E7, and CJY/S3 and Acanthamoeba lenticulata 68–2 were 0.31, 0.2, and 0.26%, respectively. 18S ribosomal deoxyribonucleic acid (rDNA) of CJY/W1 had 99% sequence identity to that of Acanthamoeba sp. U/H-C1. Strains CJY/S1 and CJY/S2, isolated from soil, had similar mtDNA RFLP patterns, whereas strain CJY/W1, isolated from tap water, displayed a different pattern.

Conclusions

To the best of our knowledge, this is the first report on the identification of genotypes T4, T5, and T16 from environmental sources in Yanji, China.
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Metadata
Title
Isolation and identification of Acanthamoeba strains from soil and tap water in Yanji, China
Authors
Yinghua Xuan
Yanqin Shen
Yuxi Ge
Gen Yan
Shanzi Zheng
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Environmental Health and Preventive Medicine / Issue 1/2017
Print ISSN: 1342-078X
Electronic ISSN: 1347-4715
DOI
https://doi.org/10.1186/s12199-017-0655-2

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