Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

ACC 2024 Congress

Catch up on all the top stories and latest evidence in cardiology research with our ACC 2024 Congress hub page.
ADVANCED SEARCH
Log in
Top
Environmental Health and Preventive Medicine
Published in: Environmental Health and Preventive Medicine 1/2017

Open Access 01-12-2017 | Research article

Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections

Authors: Zahra Shamsizadeh, Mahnaz Nikaeen, Bahram Nasr Esfahani, Seyed Hamed Mirhoseini, Maryam Hatamzadeh, Akbar Hassanzadeh

Published in: Environmental Health and Preventive Medicine | Issue 1/2017

Login to get access

Abstract

Background

Antibiotic resistant Acinetobacter baumannii has emerged as one of the most problematic hospital acquired pathogens around the world. This study was designed to investigate the presence of antibiotic resistant A. baumannii in various hospital environments.

Methods

Air, water and inanimate surface samples were taken in different wards of four hospitals and analyzed for the presence of A. baumannii. Confirmed A. baumannii isolates were analyzed for antimicrobial susceptibility and also screened for the presence of three most common OXA- type carbapenemase-encoding genes.

Results

A. baumannii was detected in 11% (7/64) of air samples with the highest recovery in intensive care units (ICUs). A. baumannii was also detected in 17% (7/42) and 2% (1/42) of surface and water samples, respectively. A total of 40 A. baumannii isolates were recovered and analysis of antimicrobial susceptibility showed the highest resistance towards ceftazidime (92.5%, 37/40). 85% (34/40) and 80% (32/40) of the isolates were also resistant to imipenem and gentamicin, respectively. Resistance genes analysis showed that 77.5% (31/40) strains contained OXA-23 and 5% (2/40) strains contained OXA-24, but OXA-58 was not detected in any of the strains.

Conclusion

Detection of antibiotic resistant A. baumannii in various samples revealed that hospital environments could act as a potential source for transmission of A. baumannii infections especially in ICUs. These results emphasize the importance of early detection and implementation of control measures to prevent the spread of A. baumannii in hospital environments.
Literature
1.
Kilic A, Li H, Mellmann A, et al. Acinetobacter septicus sp. nov. association with a nosocomial outbreak of bacteremia in a neonatal intensive care unit. J Clin Microbiol. 2008;46:902–8.CrossRefPubMedPubMedCentral
2.
Beggs C. The airborne transmission of infection in hospital buildings: fact or fiction? Indoor Built Environ. 2003;12:9–18.CrossRef
3.
4.
Custovic A, Smajlovic J, Tihic N, Hadzic S, Ahmetagic S, Hadzagic H. Epidemiological Monitoring of Nosocomial Infections Caused by Acinetobacter Baumannii. Med Arh. 2014;68:402–6.CrossRef
5.
Fournier PE, Richet H, Weinstein RA. The epidemiology and control of Acinetobacter baumannii in health care facilities. Clin Infect Dis. 2006;42:692–9.CrossRefPubMed
6.
Bergogne-Berezin E, Towner K. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev. 1996;9:148–65.PubMedPubMedCentral
7.
Cicek AC, Karagoz A, Koksal E, et al. A Single Clone Acinetobacter baumannii Outbreak in a State Hospital in Turkey. Jpn J Infect Dis. 2013;66:245–8.CrossRefPubMed
8.
Benedetta L, Annalisa P, Ida L, et al. Molecular findings and antibiotic-resistance in an outbreak of Acinetobacter baumannii in an intensive care unit. Ann Ist Super Sanita. 2007;43(1):83–8.
9.
Levy SB, Marshall B. Antibacterial resistance worldwide: causes, challenges and responses. Nat Med. 2004;10:S122–9.CrossRefPubMed
10.
Pajand O, Rezaee MA, Nahaei MR, Mahdian R, Aghazadeh M, Soroush MH, et al. Study of the carbapenem resistance mechanisms in clinical isolates of Acinetobacter baumannii: Comparison of burn and non-burn strains. Burns. 2013;39:1414–9.CrossRefPubMed
11.
Akalin H, Özakin C, Gedikoğlu S. Epidemiology of Acinetobacter baumannii in a university hospital in Turkey. Infect Control. 2006;27:404–8.
12.
Gao J, Zhao X, Bao Y, et al. Antibiotic resistance and OXA-type carbapenemases-encoding genes in airborne Acinetobacter baumannii isolated from burn wards. Burns. 2014;40:295–9.CrossRefPubMed
13.
Wang S, Sheng W, Chang Y, et al. Healthcare-associated outbreak due to pan-drug resistant Acinetobacter baumannii in a surgical intensive care unit. J Hosp Infect. 2003;53:97–102.CrossRefPubMed
14.
Munoz-Price LS, Fajardo-Aquino Y, Arheart KL, et al. Aerosolization of Acinetobacter baumannii in a trauma ICU. Crit Care Med. 2013;41:1915–8.CrossRefPubMed
15.
CLSI. Performance Standards for Antimicribial Sucseptibility Testing. Twenty- Second Informational Supplement. Wayne: CLSI document M100-S22; 2012.
16.
Bou G, Cervero G, Dominguez M, Quereda C, Martínez‐Beltrán J. PCR‐based DNA fingerprinting (REP‐PCR, AP‐PCR) and pulsed‐field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem‐and meropenem‐resistant Acinetobacter baumannii. Clin Microbiol Infect. 2000;6:635–43.CrossRefPubMed
17.
Weinstein RA, Hota B. Contamination, disinfection, and cross-colonization: are hospital surfaces reservoirs for nosocomial infection? Clin Infect Dis. 2004;39:1182–9.CrossRef
18.
Murray G, Peleg A, editors. Acinetobacter baumannii: Evolution of Antimicrobial Resistance-Treatment Options. Seminars in respiratory and critical care medicine. 2015.
19.
Sarıca S, Asan A, Otkun MT, Ture M. Monitoring indoor airborne fungi and bacteria in the different areas of Trakya University Hospital, Edirne, Turkey. Indoor Built Environ. 2002;11:285–92.CrossRef
20.
Bernards AT, Frénay HM, Lim BT, Hendriks WD, Dijkshoorn L, van Boven CP. Methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii: an unexpected difference in epidemiologic behavior. Am J Infect Control. 1998;26:544–51.CrossRefPubMed
21.
Tang J, Li Y, Eames I, Chan P, Ridgway G. Factors involved in the aerosol transmission of infection and control of ventilation in healthcare premises. J Hosp Infect. 2006;64:100–14.CrossRefPubMed
22.
Asghari FB, Nikaeen M, Hatamzadeh M, Hassanzadeh A. Surveillance of Legionella species in hospital water systems: the significance of detection method for environmental surveillance data. J Water Health. 2013;11:713–9.CrossRefPubMed
23.
Asghari FB, Nikaeen M, Mirhendi H. Rapid monitoring of Pseudomonas aeruginosa in hospital water systems: a key priority in prevention of nosocomial infection. FEMS Microbiol Lett. 2013;343:77–81.CrossRef
24.
Aygün G, Demirkiran O, Utku T, et al. Environmental contamination during a carbapenem-resistant Acinetobacter baumannii outbreak in an intensive care unit. J Hosp Infect. 2002;52:259–62.CrossRefPubMed
25.
Tena D, Martínez NM, Oteo J, et al. Outbreak of multiresistant OXA-24-and OXA-51-producing Acinetobacter baumannii in an internal medicine ward. Jpn J Infect Dis. 2013;66:323–6.CrossRefPubMed
26.
Lautenbach E, Synnestvedt M, Weiner MG, et al. Epidemiology and impact of imipenem resistance in Acinetobacter baumannii. Infect Control. 2009;30:1186–92.
27.
Lemos E, de la Hoz F, Einarson T, et al. Carbapenem resistance and mortality in patients with Acinetobacter baumannii infection: systematic review and meta‐analysis. Clin Microbiol Infect. 2014;20:416–23.CrossRefPubMed
28.
Cai Y, Chai D, Wang R, Liang B, Bai N. Colistin resistance of Acinetobacter baumannii: clinical reports, mechanisms and antimicrobial strategies. J Antimicrob Chemother. 2012;67:1607–15.CrossRefPubMed
29.
D'Arezzo S, Principe L, Capone A, Petrosillo N, Petrucca A, Visca P. Changing carbapenemase gene pattern in an epidemic multidrug-resistant Acinetobacter baumannii lineage causing multiple outbreaks in central Italy. J Antimicrob Chemother. 2011;66:54–61.CrossRefPubMed
30.
Vanbroekhoven K, Ryngaert A, Wattiau P, De Mot R, Springael D. Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR–DGGE fingerprinting. FEMS Microbiol Ecol. 2004;50:37–50.CrossRefPubMed
31.
Woodford N, Ellington MJ, Coelho JM, Turton JF, Ward ME, Brown S, et al. Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents. 2006;27:351–3.CrossRefPubMed
Metadata
Title
Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections
Authors
Zahra Shamsizadeh
Mahnaz Nikaeen
Bahram Nasr Esfahani
Seyed Hamed Mirhoseini
Maryam Hatamzadeh
Akbar Hassanzadeh
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Environmental Health and Preventive Medicine / Issue 1/2017
Print ISSN: 1342-078X
Electronic ISSN: 1347-4715
DOI
https://doi.org/10.1186/s12199-017-0653-4

Other articles of this Issue 1/2017

Environmental Health and Preventive Medicine 1/2017 Go to the issue

Letter to the Editor

Comments on “Association of excessive mobile phone use during pregnancy with birth weight: an adjunct study in Kumamoto of Japan Environment and Children’s Study”

Regular Article

Occupation exposed to road-traffic emissions and respiratory health among Congolese transit workers, particularly bus conductors, in Kinshasa: a cross-sectional study

Regular Article

Comorbidities according to airflow limitation severity: data from comprehensive health examination in Japan

Short communication

Paraben exposure and semen quality of Japanese male partners of subfertile couples

Regular Article

Influence of a 50bp Ins/Del polymorphism at promoter of the superoxide dismutase-1 on gene expression and risk of heroin dependency

Short communication

Genetic analysis of RNF213 p.R4810K variant in non-moyamoya intracranial artery stenosis/occlusion disease in a Chinese population