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Environmental Health and Preventive Medicine

Published in:

Open Access 01-12-2017 | Regular Article

Alteration in plasma free amino acid levels and its association with gout

Authors: MH Mahbub, Natsu Yamaguchi, Hidekazu Takahashi, Ryosuke Hase, Hiroki Amano, Mikiko Kobayashi-Miura, Hideyuki Kanda, Yasuyuki Fujita, Hiroshi Yamamoto, Mai Yamamoto, Shinya Kikuchi, Atsuko Ikeda, Naoko Kageyama, Mina Nakamura, Yasutaka Ishimaru, Hiroshi Sunagawa, Tsuyoshi Tanabe

Published in: Environmental Health and Preventive Medicine | Issue 1/2017

Abstract

Background

Studies on the association of plasma-free amino acids with gout are very limited and produced conflicting results. Therefore, we sought to explore and characterize the plasma-free amino acid (PFAA) profile in patients with gout and evaluate its association with the latter.

Methods

Data from a total of 819 subjects (including 34 patients with gout) undergoing an annual health examination program in Shimane, Japan were considered for this study. Venous blood samples were collected from the subjects and concentrations of 19 plasma amino acids were determined by high-performance liquid chromatography–electrospray ionization–mass spectrometry. Student’s t-test was applied for comparison of variables between patient and control groups. The relationships between the presence or absence of gout and individual amino acids were investigated by logistic regression analysis controlling for the effects of potential demographic confounders.

Results

Among 19 amino acids, the levels of 10 amino acids (alanine, glycine, isoleucine, leucine, methionine, phenylalanine, proline, serine, tryptophan, valine) differed significantly (P < .001 to .05) between the patient and control groups. Univariate logistic regression analysis revealed that plasma levels of alanine, isoleucine, leucine, phenylalanine, tryptophan and valine had significant positive associations (P < .005 to .05) whereas glycine and serine had significant inverse association (P < .05) with gout.

Conclusions

The observed significant changes in PFAA profiles may have important implications for improving our understanding of pathophysiology, diagnosis and prevention of gout. The findings of this study need further confirmation in future large-scale studies involving a larger number of patients with gout.

Kramer HM, Curhan G. The association between gout and nephrolithiasis: the National Health and Nutrition Examination Survey III, 1988–1994. Am J Kidney Dis. 2002;40(1):37–42.CrossRefPubMed

Kuo CF, Grainge MJ, Mallen C, Zhang W, Doherty M. Rising burden of gout in the UK but continuing suboptimal management: a nationwide population study. Ann Rheum Dis. 2015;74(4):661–7.CrossRefPubMed

Doherty M. New insights into the epidemiology of gout. Rheumatology (Oxford). 2009;48 Suppl 2:ii2–8.CrossRef

Kawasaki T, Shichikawa K. Epidemiology survey of gout using residents’ health checks. Gout Nucleic Acid Metabo. 2006;30:66 (in Japanese).

Kuo CF, Grainge MJ, Zhang W, Doherty M. Global epidemiology of gout: prevalence, incidence and risk factors. Nat Rev Rheumatol. 2015;11(11):649–62.CrossRefPubMed

Roddy E, Zhang W, Doherty M. The changing epidemiology of gout. Nat Clin Pract Rheumatol. 2007;3(8):443–9.CrossRefPubMed

Smith E, Hoy D, Cross M, Merriman TR, Vos T, Buchbinder R, Woolf A, March L. The global burden of gout: estimates from the global burden of disease 2010 study. Ann Rheum Dis. 2014;73(8):1470–6.CrossRefPubMed

Scott JT. New knowledge of the pathogenesis of gout. J Clin Pathol Suppl (R Coll Pathol). 1978;12:205–13.CrossRef

Maiuolo J, Oppedisano F, Gratteri S, Muscoli C, Mollace V. Regulation of uric acid metabolism and excretion. Int J Cardiol. 2016;213:8–14.CrossRefPubMed

10.

Yü TF, Adler M, Bobrow E, Gutman AB. Plasma and urinary amino acids in primary gout, with special reference to glutamine. J Clin Invest. 1969;48(5):885–94.CrossRefPubMedPubMedCentral

11.

Miyagi Y, Higashiyama M, Gochi A, Akaike M, Ishikawa T, Miura T, Saruki N, Bando E, Kimura H, Imamura F, Moriyama M, Ikeda I, Chiba A, Oshita F, Imaizumi A, Yamamoto H, Miyano H, Horimoto K, Tochikubo O, Mitsushima T, Yamakado M, Okamoto N. Plasma free amino acid profiling of five types of cancer patients and its application for early detection. PLoS One. 2011;6(9):e24143.CrossRefPubMedPubMedCentral

12.

Nakamura H, Nishikata N, Kawai N, Imaizumi A, Miyano H, Mori M, Yamamoto H, Noguchi Y. Plasma amino acid profiles in healthy East Asian subpopulations living in Japan. Am J Hum Biol. 2016;28(2):236–9.CrossRefPubMed

13.

Tochikubo O, Nakamura H, Jinzu H, Nagao K, Yoshida H, Kageyama N, Miyano H. Weight loss is associated with plasma free amino acid alterations in subjects with metabolic syndrome. Nutr Diabetes. 2016;6:e197.CrossRefPubMedPubMedCentral

14.

Yamakado M, Tanaka T, Nagao K, Ishizaka Y, Mitushima T, Tani M, Toda A, Toda E, Okada M, Miyano H, Yamamoto H. Plasma amino acid profile is associated with visceral fat accumulation in obese Japanese subjects. Clin Obes. 2012;2(1–2):29–40.CrossRefPubMed

15.

Hisamatsu T, Okamoto S, Hashimoto M, Muramatsu T, Andou A, Uo M, Kitazume MT, Matsuoka K, Yajima T, Inoue N, Kanai T, Ogata H, Iwao Y, Yamakado M, Sakai R, Ono N, Ando T, Suzuki M, Hibi T. Novel, objective, multivariate biomarkers composed of plasma amino acid profiles for the diagnosis and assessment of inflammatory bowel disease. PLoS One. 2012;7:e31131.CrossRefPubMedPubMedCentral

16.

Yü TF, Kaung C, Gutman AB. Effect of glycine loading on plasma and urinary uric acid and amino acids in normal and gouty subjects. Am J Med. 1970;49(3):352–9.CrossRefPubMed

17.

Derrick JB, Hanley AP. Amino acids in the blood and urine of normal and arthritic subjects before and after a glycine load given with and without adrenocorticotropin. Canad J Biochem. 1957;35:1005–16.CrossRefPubMed

18.

Kaplan D, Bernstein D, Wallace SL, Halberstam D. Serum and urinary amino acids in normouricemic and hyperuricemic subjects. Ann Intern Med. 1965;62:658–66.CrossRefPubMed

19.

Shimbo K, Kubo S, Harada Y, Oonuki T, Yokokura T, Yoshida H, Amao M, Nakamura M, Kageyama N, Yamazaki J, Ozawa S, Hirayama K, Ando T, Miura J, Miyano H. Automated precolumn derivatization system for analyzing physiological amino acids by liquid chromatography/mass spectrometry. Biomed Chromatogr. 2010;24(7):683–91.CrossRefPubMed

20.

Shimbo K, Oonuki T, Yahashi A, Hirayama K, Miyano H. Precolumn derivatization reagents for high-speed analysis of amines and amino acids in biological fluid using liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 2009;23(10):1483–92.CrossRefPubMed

21.

Yamakado M, Nagao K, Imaizumi A, Tani M, Toda A, Tanaka T, Jinzu H, Miyano H, Yamamoto H, Daimon T, Horimoto K, Ishizaka Y. Plasma free amino acid profiles predict four-year risk of developing diabetes, metabolic syndrome, dyslipidemia, and hypertension in japanese population. Sci Rep. 2015;9(5):11918.CrossRef

22.

Nagahama K, Iseki K, Inoue T, Touma T, Ikemiya Y, Takishita S. Hyperuricemia and cardiovascular risk factor clustering in a screened cohort in Okinawa, Japan. Hypertens Res. 2004;27:227–33.CrossRefPubMed

23.

Grundy SM. Gamma-glutamyl transferase: another biomarker for metabolic syndrome and cardiovascular risk. Arterioscler Thromb Vasc Biol. 2007;27:4–7.CrossRefPubMed

24.

Chen SC, Huang YF, Wang JD. Hyperferritinemia and hyperuricemia may be associated with liver function abnormality in obese adolescents. PLoS One. 2012;7:e48645.CrossRefPubMedPubMedCentral

25.

Pagliara AS, Goodman AD. Elevation of plasma glutamate in gout. Its possible role in the pathogenesis of hyperuricemia. New Eng J Med. 1969;281:767.CrossRefPubMed

26.

Armstrong MD, Stave U. A study of plasma free amino acid levels. V. Correlations among the amino acids and between amino acids and some other blood constituents. Metabolism. 1973;22(6):827–33.CrossRefPubMed

Title: Alteration in plasma free amino acid levels and its association with gout
Authors: MH Mahbub
Natsu Yamaguchi
Hidekazu Takahashi
Ryosuke Hase
Hiroki Amano
Mikiko Kobayashi-Miura
Hideyuki Kanda
Yasuyuki Fujita
Hiroshi Yamamoto
Mai Yamamoto
Shinya Kikuchi
Atsuko Ikeda
Naoko Kageyama
Mina Nakamura
Yasutaka Ishimaru
Hiroshi Sunagawa
Tsuyoshi Tanabe
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Environmental Health and Preventive Medicine / Issue 1/2017
Print ISSN: 1342-078X
Electronic ISSN: 1347-4715
DOI: https://doi.org/10.1186/s12199-017-0609-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Alteration in plasma free amino acid levels and its association with gout

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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