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Open Access 01-12-2016 | Research article

Expression of artemin and GFRα3 in an animal model of migraine: possible role in the pathogenesis of this disorder

Authors: Hai-Qiong Shang, Yan Wang, Yan-Yan Mao, Li-Gang Kong, Gao-Ying Sun, Lei Xu, Dao-Gong Zhang, Yue-Chen Han, Jian-Feng Li, Hai-Bo Wang, Zhao-Min Fan

Published in: The Journal of Headache and Pain | Issue 1/2015

Abstract

Background

Neurotrophic factors have been implicated in hyperalgesia and peripheral levels of these molecules are altered in migraine pathophysiology. Artemin, a vasculature-derived neurotrophic factor, contributes to pain modulation and trigeminal primary afferent sensitization through binding its selective receptor GFRα3. The distribution of artemin and GFRα3 in the dura mater raises an anatomy supports that they may be involved in migraine. In this study we evaluated the expression of artemin and GFRα3 in an animal migraine model that may be relevant for migraine.

Methods

In this study, using a rat migraine model by administration of nitroglycerin (NTG), we investigated the expression of artemin in the dura mater and GFRα3 in the trigeminal ganglia (TG) by means of quantitative reverse transcription-polymerase chain reaction, western blot and immunofluorescence labeling.

Results

Artemin immunoreactivity was found in the smooth muscle cells of dural vasculature and GFRα3 was present in cytoplasm of TG neurons. The mRNA levels of artemin and GFRα3 were significantly elevated after NTG treatment at 2 and 4 h respectively (P < 0.05). The expression of artemin protein was increased at 4 h and continually up to 8 h in the dura mater following NTG administration (P < 0.05). The expression of GFRα3 protein was elevated at 4 h and continually up to 10 h in the TG following NTG administration (P < 0.05).

Conclusion

The findings suggest that artemin and GFRα3 play an important role in the pathogenesis of migraine and may represent potential therapeutic targets for the treatment of migraine.

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Title: Expression of artemin and GFRα3 in an animal model of migraine: possible role in the pathogenesis of this disorder
Authors: Hai-Qiong Shang
Yan Wang
Yan-Yan Mao
Li-Gang Kong
Gao-Ying Sun
Lei Xu
Dao-Gong Zhang
Yue-Chen Han
Jian-Feng Li
Hai-Bo Wang
Zhao-Min Fan
Publication date: 01-12-2016
Publisher: Springer Milan
Published in: The Journal of Headache and Pain / Issue 1/2015
Print ISSN: 1129-2369
Electronic ISSN: 1129-2377
DOI: https://doi.org/10.1186/s10194-016-0673-2

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Expression of artemin and GFRα3 in an animal model of migraine: possible role in the pathogenesis of this disorder

Abstract

Background

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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