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Critical Care
Published in: Critical Care 1/2011

Open Access 01-02-2011 | Research

High levels of circulating leukocyte microparticles are associated with better outcome in acute respiratory distress syndrome

Authors: Christophe Guervilly, Romaric Lacroix, Jean-Marie Forel, Antoine Roch, Laurence Camoin-Jau, Laurent Papazian, Françoise Dignat-George

Published in: Critical Care | Issue 1/2011

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Abstract

Introduction

The current study has addressed the presence and the cellular origin of microparticles (MP) isolated from bronchoalveolar lavage (BAL) fluid and from blood samples from patients with acute respiratory distress syndrome (ARDS). Their prognostic interest was also investigated.

Methods

Fifty-two patients were included within the first 24 hours of ARDS. They were compared to spontaneous breathing (SB) and ventilated control (VC) groups. Bronchoalveolar lavage (BAL) and blood samples were obtained on Day 1 and Day 3 in an ARDS group. Leukocyte microparticles (LeuMP), neutrophil microparticles (NeuMP), endothelial microparticles (EMP), and platelet microparticles (PMP) were measured in arterial blood and in BAL samples by flow cytometry. Mortality from all causes was recorded at Day 28.

Results

All MP subpopulations were detected in BAL. However, only LeuMP and NeuMP were elevated in ARDS patients compared to the SB group (P = 0.002 for both). Among ARDS patients, higher levels of LeuMP were detected in blood (Day 1) and in BAL (Day 3) in survivors as compared with the non survivors. Circulating LeuMP >60 elements/microliter detectable on Day 1 of ARDS, was associated with a higher survival rate (odds ratio, 5.26; 95% confidence interval, 1.10 to 24.99; P = 0.037).

Conclusions

The identification of the cellular origin of microparticles at the onset of ARDS has identified LeuMP as a biomarker of prognostic significance. The higher levels of LeuMP in survivors could be associated with a protective role of this MP subpopulation. This hypothesis needs further investigations.
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Metadata
Title
High levels of circulating leukocyte microparticles are associated with better outcome in acute respiratory distress syndrome
Authors
Christophe Guervilly
Romaric Lacroix
Jean-Marie Forel
Antoine Roch
Laurence Camoin-Jau
Laurent Papazian
Françoise Dignat-George
Publication date
01-02-2011
Publisher
BioMed Central
Published in
Critical Care / Issue 1/2011
Electronic ISSN: 1364-8535
DOI
https://doi.org/10.1186/cc9978

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