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Open Access 01-08-2000 | Research

Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid ‘real-time’ polymerase chain reaction

Authors: Jean-Paul Pirnay, Daniel De Vos, Luc Duinslaeger, Pascal Reper, Christian Vandenvelde, Pierre Cornelis, Alain Vanderkelen

Published in: Critical Care | Issue 4/2000

Statement of findings

We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 10³ colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.

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Title: Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid ‘real-time’ polymerase chain reaction
Authors: Jean-Paul Pirnay
Daniel De Vos
Luc Duinslaeger
Pascal Reper
Christian Vandenvelde
Pierre Cornelis
Alain Vanderkelen
Publication date: 01-08-2000
Publisher: BioMed Central
Published in: Critical Care / Issue 4/2000
Electronic ISSN: 1364-8535
DOI: https://doi.org/10.1186/cc702

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid ‘real-time’ polymerase chain reaction

Statement of findings

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Statement of findings

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