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Critical Care
Published in: Critical Care 3/2008

Open Access 01-06-2008 | Research

Effects of salbutamol on exhaled breath condensate biomarkers in acute lung injury: prospective analysis

Authors: Oriol Roca, Susana Gómez-Ollés, Maria-Jesús Cruz, Xavier Muñoz, Mark JD Griffiths, Joan R Masclans

Published in: Critical Care | Issue 3/2008

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Abstract

Introduction

The benefits of β-adrenergic stimulation have been described in acute lung injury (ALI), but there is still no evidence of its anti-inflammatory effect in these patients. Biomarkers in exhaled breath condensate (EBC) were used to study the effects of salbutamol on lung inflammation in mechanically ventilated patients with ALI.

Methods

EBC was collected before and 30 minutes after administration of inhaled salbutamol (800 μg). The following parameters were measured in the samples: volume obtained, conductivity, pH after helium deaeration, and concentration of nitrites, nitrates and 8-isoprostane. The leukotriene B4 concentration was measured after sample lyophilization and reconstitution. Results are expressed as the median (interquartile range).

Results

EBC was obtained from six ALI patients, with a median age of 56 (46 to 76) years. At the time of EBC collection, the Lung Injury Score was 3 (2.3 to 3.1) and the PaO2/FIO2 ratio was 133 (96 to 211) mmHg. A significant increase in deaerated EBC pH was observed after salbutamol administration (7.66 (7.58 to 7.75) versus 7.83 (7.67 to 7.91), P = 0.028). Trends toward decreased nitrosative species (18.81 (13.33 to 49.44) μM versus 21.21 (8.07 to 29.83) μM, P = 0.173) and decreased 8-isoprostane concentration (11.64 (7.17 to 17.13) pg/ml versus 6.55 (4.03 to 9.99) pg/ml, P = 0.068) were detected. No changes in leukotriene B4 concentration were found (1.58 (0.47 to 3.57) pg/ml versus 2.06 (1.01 to 3.01) pg/ml, P = 0.753).

Conclusion

EBC analysis is a noninvasive technique that can be used to monitor ventilated patients. In EBC from a small cohort of patients with ALI, inhaled salbutamol significantly decreased airspace acidosis, a marker of inflammation, and was associated with a trend toward decreased markers of nitrosative and oxidative stress.
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Metadata
Title
Effects of salbutamol on exhaled breath condensate biomarkers in acute lung injury: prospective analysis
Authors
Oriol Roca
Susana Gómez-Ollés
Maria-Jesús Cruz
Xavier Muñoz
Mark JD Griffiths
Joan R Masclans
Publication date
01-06-2008
Publisher
BioMed Central
Published in
Critical Care / Issue 3/2008
Electronic ISSN: 1364-8535
DOI
https://doi.org/10.1186/cc6911

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