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Critical Care
Published in: Critical Care 3/2006

Open Access 01-06-2006 | Research

Influence of fluid resuscitation on renal microvascular PO2 in a normotensive rat model of endotoxemia

Authors: Tanja Johannes, Egbert G Mik, Boris Nohé, Nicolaas JH Raat, Klaus E Unertl, Can Ince

Published in: Critical Care | Issue 3/2006

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Abstract

Introduction

Septic renal failure is often seen in the intensive care unit but its pathogenesis is only partly understood. This study, performed in a normotensive rat model of endotoxemia, tests the hypotheses that endotoxemia impairs renal microvascular PO2 (μPO2) and oxygen consumption (VO2,ren), that endotoxemia is associated with a diminished kidney function, that fluid resuscitation can restore μPO2, VO2,ren and kidney function, and that colloids are more effective than crystalloids.

Methods

Male Wistar rats received a one-hour intravenous infusion of lipopolysaccharide, followed by resuscitation with HES130/0.4 (Voluven®), HES200/0.5 (HES-STERIL® ® 6%) or Ringer's lactate. The renal μPO2 in the cortex and medulla and the renal venous PO2 were measured by a recently published phosphorescence lifetime technique.

Results

Endotoxemia induced a reduction in renal blood flow and anuria, while the renal μPO2 and VO2,ren remained relatively unchanged. Resuscitation restored renal blood flow, renal oxygen delivery and kidney function to baseline values, and was associated with oxygen redistribution showing different patterns for the different compounds used. HES200/0.5 and Ringer's lactate increased the VO2,ren, in contrast to HES130/0.4.

Conclusion

The loss of kidney function during endotoxemia could not be explained by an oxygen deficiency. Renal oxygen redistribution could for the first time be demonstrated during fluid resuscitation. HES130/0.4 had no influence on the VO2,ren and restored renal function with the least increase in the amount of renal work.
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Metadata
Title
Influence of fluid resuscitation on renal microvascular PO2 in a normotensive rat model of endotoxemia
Authors
Tanja Johannes
Egbert G Mik
Boris Nohé
Nicolaas JH Raat
Klaus E Unertl
Can Ince
Publication date
01-06-2006
Publisher
BioMed Central
Published in
Critical Care / Issue 3/2006
Electronic ISSN: 1364-8535
DOI
https://doi.org/10.1186/cc4948

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