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Breast Cancer Research
Published in: Breast Cancer Research 1/2013

Open Access 01-02-2013 | Research article

RhoB modifies estrogen responses in breast cancer cells by influencing expression of the estrogen receptor

Authors: Claire Médale-Giamarchi, Isabelle Lajoie-Mazenc, Emilie Malissein, Elise Meunier, Bettina Couderc, Yann Bergé, Thomas Filleron, Laura Keller, Claudine Marty, Magali Lacroix-Triki, Florence Dalenc, Sophie F Doisneau-Sixou, Gilles Favre

Published in: Breast Cancer Research | Issue 1/2013

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Abstract

Introduction

RhoB has been reported to exert positive and negative effects on cancer pathophysiology but an understanding of its role in breast cancer remains incomplete. Analysis of data from the Oncomine database showed a positive correlation between RhoB expression and positivity for both estrogen receptor alpha (ERα) and progesterone receptor (PR).

Methods

This finding was validated by our analysis of a tissue microarray constructed from a cohort of 113 patients and then investigated in human cell models.

Results

We found that RhoB expression in tissue was strongly correlated with ERα and PR expression and inversely correlated with tumor grade, tumor size and count of mitosis. In human breast cancer cell lines, RhoB attenuation was associated with reduced expression of both ERα and PR, whereas elevation of RhoB was found to be associated with ERα overexpression. Mechanistic investigations suggested that RhoB modulates ERα expression, controlling both its protein and mRNA levels, and that RhoB modulates PR expression by accentuating the recruitment of ERα and other major co-regulators to the promoter of PR gene. A major consequence of RhoB modulation was that RhoB differentially regulated the proliferation of breast cancer cell lines. Interestingly, we documented crosstalk between RhoB and ERα, with estrogen treatment leading to RhoB activation.

Conclusion

Taken together, our findings offer evidence that in human breast cancer RhoB acts as a positive function to promote expression of ERα and PR in a manner correlated with cell proliferation.
Appendix
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Metadata
Title
RhoB modifies estrogen responses in breast cancer cells by influencing expression of the estrogen receptor
Authors
Claire Médale-Giamarchi
Isabelle Lajoie-Mazenc
Emilie Malissein
Elise Meunier
Bettina Couderc
Yann Bergé
Thomas Filleron
Laura Keller
Claudine Marty
Magali Lacroix-Triki
Florence Dalenc
Sophie F Doisneau-Sixou
Gilles Favre
Publication date
01-02-2013
Publisher
BioMed Central
Published in
Breast Cancer Research / Issue 1/2013
Electronic ISSN: 1465-542X
DOI
https://doi.org/10.1186/bcr3377

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